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- Abstract：In order to study the perception of GMO risk and safety among Chinese public,we conducted a questionnaire survey in 30 provinces/cities around China.Analysis of over 4 000 questionnaires reveals that:the low perception of GMO among Chinese public,the Chinese public is extremely cautious about the safety of GMO food and that they do not have a clear recognition of bio-safety.Therefore,it is necessary to reinforce the popularization of GMO and to establish communication platform on GMO risks,and the government and research institutions should take the responsibility of the popularization of GMO knowledge.At the same time,it is very important to strengthen scientific popularization and management,to establish exchange platform for GMO risks,and to pay special attention to the communications with young people,especially with those higher educational level youth.Keywords：GMO;risk communication;bio-safety;public survey;questionnaire11|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：With the analysis of the questionnaires, we prove that it is of great significance for the development of GMO industry to set up scientific and sound risk communications against GMO risks.After exploring ways of risk communications between any two of the following five subjects-government,enterprises,media,scientists and the public,this article illustrates the best way and delineates the frame of mechanism and measures of GMO risk communications.Meanwhile,the obligations and responsibilities of each of the five subjects are also clarified in the paper.Finally,we suggested that an administrative institution combined with multiple related government departments should be set up to be in the charge of management and coordination of GMO risk communications.We also suggested that the government should facilitate the perception and popularizing of GMO through television,internet,newspaper and periodicals,mainly focusing on issues connected closely with the public health.Keywords：GMO;biological safety;risks communication;questionnaire3|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This study was to analyze the effects of different concentrations of two maize (transgenic Bt maize and non-transgenic maize) straw extract on germination and seedling growth of wheat.All experiments were investigated by means of bioassay in laboratory.The results showed that both maize straw extract at low concentration (1∶100) had no significant effect on wheat germination while high concentration (1∶25) inhibited seed germination,especially treated by transgenic Bt maize straw extract.At 1∶100 concentration transgenic Bt maize straw extract significantly increased seedling height and root length,which reached 9.7 and 10.84 cm respectively.However,non-transgenic maize straw extract at concentration of 1∶25 significant decreased seedling height (6.9 cm) and root length (4.28 cm),transgenic Bt maize straw could release Bt protein into aqueous extract,but there was no influence on wheat germination when seed soaked in Bt solution for 24 h.Therefore,a certain proportion of Bt-maize straw ploughed into soil has no adverse affect on germination of the following crop.Keywords：wheat;germination percentage;Bt protein;straw aqueous extracts;seedlings growth;maize2|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The genetic transformation of wheat Mianyang 19 and Luoyang 8716 immature embryo callus mediated by Agrobacterium tumefaciens was studied.The factors that influence the efficiency of transformation were investigated by using the transient expression of gus gene.The result showed that for both varieties,the best transformation procedure of EHA105 was Agrobacterium cell density of OD600≈0.8,incubation for 30 min.When using Agrobacterium LBA4404,the transformation efficiency of Mianyang 19 and Luoyang 8716 immature embryo callus was highest when cell density was OD600≈1.0,incubation for 30 min and cell density of OD600≈0.8,incubation for 1h,respectively.Using the optimized conditions,the high molecular weight glutenin subunit 1Bx14 gene was transformed.After selected with hygromycin,tested by PCR and PCR-Southern,the positive progenies were obtained,yielding an average transformation frequency of 0.61%.Keywords：wheat;Agrobacterium tumefaciens;transformation;high molecular weight glutenin subunit1|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Maize is one of the most important crops in China.With the development of molecular biology,molecular breeding is becoming one of the hot spots in maize genetics and improvement.The rapid method of DNA extraction with high efficiency is the first step in large-scale molecular breeding.Therefore,developing a rapid DNA extraction method for large maize populations is necessary to meet the need of the small labs in China.In this study,a new method of extracting DNA for genotyping large populations was developed.Firstly,kernel endosperm tissue in maize was used to extract DNA by using the improved alkali-boiling method;secondly,tender leaf tissue in maize was used to extract DNA by utilizing electric drill milling and simplified CTAB method.These two methods were combined to prepare DNA for genotyping before and after planting.Compared with the conventional ways of genotyping in large populations,this new method with higher efficiency and lower costs could be widely used in maize molecular breeding.Keywords：maize;large populations;endosperm;alkali-boiling method;rapid extraction5|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Field experiments were conducted in Gongzhuling and Huadian of Jilin Province in order to analyze the influence of ecological factors and different densities on the yield and the yield components.Five planting densities were applied 40 000,50 000,60 000,70 000 and 80 000 plant/hm2.The results were as follows:1) In Gongzhuling,grain yield and grain numbers per ear were not affected with the increase of planting density,while the 1 000-grain weight decreased significantly.In Huadian,grain yield significantly increased with the increase of planting density,while grain number per ear decreased.Both of the two areas obtained a maximum yield of 12 815 and 11 453 kg/hm2 at 80 000 plant/hm2.2) Ecological factors greatly affected the yield and 1 000-grain weight of maize,but had little effect on the number of ears per mu or grains per ear.In addition,the yield and 1 000-grain weight was significantly correlated with the accumulated temperatures.It was demonstrated that the lack of heat was the main limits to yield and 1 000-grain weight of maize.Accumulated temperature during mid-filling to maturity was critical for grain yield,and optimal daily temperature was 24.98 ℃.3) Sunshine hours during 12-leaves stage to silking in Huadian was longer than in Gongzhuling,which benefit to ears per hm2 under high density.7|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The differences and applicability of three methods for rice seed borne fungi testing were studied to provide scientific references to improve the accuracy of rice seed health testing.The seed borne fungi of japonica rice variety Chujing26,Dianyou35 and Yixiang9 which are mainly cultivated in Yunnan province were studied by using both washing test and PDA culture plate methods,and at the same time,the blotter moisture-retaining culture method was used to detect the seed born fungi of 20 rice hybrids,including Chujing26,Dianyou35,and Yixiang9 which were from production areas with different altitude.The results showed that washing test was applicable to detect the fungi on seed surface,the washing time was 20mins,and the washing solution with dilution ten times could be directly applied to PDA culture plate for fungi observation and counting.The Aspergillus,Fusarium,Penicillium,and Alternaria were mainly isolated from the external parts of rice seeds tested and their isolation frequency respectively were 21.4%,17.9%,16.7%,and 8.3%.PDA culture plate method was applicable to detect seed born fungi from one whole kernel or different parts of rice seed,and the test results were basically same with the results from wash test,the four major genera of fungi isolation frequency respectively were 42.7%,25.6%,1.2%,and 4.9%.There were totally 24 genera of fungi were detected from 20 rice hybrid seeds by the blotter moisture-retaining culture method,the dominant fungi were Aspergillus,Fusarium,and Penicillium which were respectively detected from 14,19 and 18 hybrids of rice seed and with high isolation frequency and widely distribution,but those three kinds of genera were failed to be detected or detected with very low isolation frequency from the seed of Banna10 and a few local rice varieties.The correlation between seed types,sources,altitude of production area,and the kinds of seed born fungi were analyzed to provide further references to identify and classify the unknown fungi isolated by above washing test,PDA culture plate and blotter moisture-retaining culture methods.Keywords：rice;mainly cultivated varieties;seed health;seed borne fungi;testing method;comparative analysis6|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In the present study,headspace solid phase microextraction (HS-SPME) combined with gas chromatography mass spectrometry (GC-MS) was employed to analyze the volatile compounds in flavedos of ‘Shatianyu’,‘Guanximiyu’,‘Yuhuanyu’,‘Cuixiangtianyu’ and ‘huyu’.The results showed that there are 75 volatile components in flavedos,and 55 kinds of alkenes take the largest part in these components,followed by 7 kinds of aldehydes,4 kinds of esters and 3 kinds of alcohols,respectively.The major volatile components are terpenes accounting for 75%-90%,and among these terpenes,β-myrcene,limonene,terpinene,α-pinene,ocimene are the major aromas.Significant differences were found in quantities,contents and categories among different kinds of volatile compounds in flavedos of "Youzi".‘Huyu’ has 10 kinds of unique components,and ‘Shatianyu’,‘Yuhuanyu’,‘Cuixiangtianyu’,‘Guanximiyu’ has 4,4,3 and 1 unique components,respectively.Keywords：pummelo;flavedo;volatile compounds;GC-MS5|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This paper is concerned on optimizing the SSR-PCR reaction system on bramble.The cultivar ‘Reveille’ of bramble was used.And the L9(34) orthogonal design and completely randomized design of two factors were used to determine the appropriate concentrations of the main factors.The best system of SSR-PCR on bramble was built and checked in 16 different bramble cultivars.The results showed that the primer concentration among the factors had highest impacts on SSR-PCR.The optimum amplification conditions of SSR-PCR were as following:Taq Polymerase 1.0 U,DNA template 30 ng,Mg2+ 1.5 mmol/L,dNTPs 0.2 mmol/L and Primer 0.4 μmol/L in 25 μL reaction volumes.With the optimum system,the amplification of 10 SSR primer pairs in 16 bramble cultivars revealed high stability.Polymorphism with 54 alleles were obtained simultaneously.It was evident that the reaction system established in this study was stable and reliable for SSR analysis of bramble,and could be used in germplasm identification,genetic polymorphism analysis and marker assisted selection.Keywords：bramble;simple sequence repeats;system optimization;application5|10|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The objective of this study was to gain better insight into the biological mechanisms of enhancing Chilling resistance tomato seedlings by AMF (arbuscular mycorrhizal fungi).Tomato variety ‘zhong za No.105’ was used.The growth and physiological indexes were measured under low temperature stress following inoculating AMF.The results indicated that low temperature stress resulted in decreases of height,fresh weight,dry weight and root-shoot ratio and the relative chlorophyll content,but increases of electrolyte permeability,soluble protein and MDA contents and,the activities of SOD and POD.The activity of CAT increased firstly and then reduced.AMF-inoculation improved the increment of plant height,the relative chlorophyll,soluble protein contents,SOD、POD and CAT activities,In contrast,the electrolyte permeability and MDA content decreased.It is therefore concluded that the cold tolerance of tomato seedlings was enhanced by AMF,probably due to alleviating damages to physiological indexes.Keywords：arbuscular mycorrhizal fungi;tomato;low temperature stress;physiological property6|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Genetic transformation system of Gladiolus cv.‘Advanced Red’ was established based on the regeneration system of embryogenic callus.Several factors were studied such as concentration of the infection,infection time,acetosyringone (AS) concentration,negative pressure and screening methods for genetic transformation of Gladiolus mediated by agrobac-terium tumefaciens(GV3101).The results showed that the highest rate of genetic transformation was obtained under the conditions of non pre-incubation,negative pressure treatment,agrobacterium concentration OD600 = 0.6-0.8,infection time 15-20 min,adding 100 μmol/LAS and 3 ds incuabtion.Following incubation for 3-4 months,the analysis of the resistant plants with PCR and southern blot indicated that the gus had been integrated into the genome of Gladiolus.Keywords：Gladiolus;gus;agrobacterium tumefaciens;genetic transformation4|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The DREBs are transcription factors which play central roles in abiotic stress response.Malus zumi, an apple rootstock,can survive 0.6% NaCl.The full-length cDNA sequence of MzDREBb gene was isolated from a cDNA library of Malus zumi by microarray analysis.The full-length cDNA of MzDREBb was 1 190 bp.Its open reading frame of 714 bp encoded a protein of 237 amino acids.The 5'-UTR and the 3'-UTR were 121 and 355 bp respectively.Base on multiple sequence alignment and phylogenetic characterization,MzDREBb was classified into A-5 subgroup of the DREB subfamily.The MzDREBb was localized in the nucleus,which exhibited transactivation activity in yeast two-hybrid system.MzDREBb was induced by either stress of high salinity or low temperature,but not by abscisic acid (ABA).Transgenic Arabidopsis over-expressing MzDREBb could enhance salt tolerance.In conclusion,MzDREBb is involved in salt response through an ABA-independent pathway in Malus zumi.3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The effect of iron nutrition on cadmium influx and accumulation in Malus xiaojinensis plants was studied using a hydroponic system.Net Cd2+ influx to the root was measured at six positions located 0.1mm to 3mm from the root tip using Cd2+ selective microelectrodes.The results showed that net Cd2+ influx decreased with increasing distance from the root tip under normal Fe level condition.In Fe deficiency,the net Cd2+ influx increased markedly in the meristematic zone and elongation zone,but changes in the maturation zone were not obvious,and the concentration of cadmium increased in shoots and roots.However,at high Fe level,the net Cd2+ influx decreased markedly in the meristematic zone and elongation zone,but also changes in the maturation zone were not obvious,and the concentration of cadmium decreased in shoots and roots.Expression studies of the Fe2+ transporter cloned from Malus xiaojinensis,MxIRT1,indicated that the expression of this transporter is distinct at different Fe levels,which might affect the transport of heavy-metal divalent cations such as Cd2+.4|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To realize accurately calculating and automatically grading of disease severity,a kind of automatic grading method of severity of single leaf infected with grape downy mildew based on image processing was proposed.In processing the completed vertical-projected images of leaf disease,leaf area and diseased area were segmented out automatically and accurately using K_means clustering (HCM) algorithm.The area features of leaf area and that of diseased area were extracted using pixels statistic.And then the assessed severity of a single leaf was obtained by calculating area ratio between diseased area and leaf area.The results show that the proposed method can assess the disease severity accurately with accuracy of 93.33%.Keywords：grape downy mildew;severity;automatic grading;image processing;K;_means clustering8|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In order to investigate the resistance of Prunus divaricata Ledeb.to Meloidogyne incognita,the resistance of Prunus divaricata Ledeb.to Meloidogyne incognita was studied with the seedlings and the method of artificial inoculation.The results showed that 0.5% inoculation perplantlet were penetreted by female adult following 30 days after artificial inoculation.Prunus divaricata Ledeb.was highly resistant to Meloidogyne incognita according to the resistance evaluation standard.Resistance was segregated in seedlings.The immuned,highly resistant,middle resistant and lowly resistant genotypes were found in Prunus divaricata Ledeb,being 30.0%,52.5%,13.8% and 3.8% of the seedlings respectively.The penetrated nematode population was 1.7% of the inoculated quantity,in which the female adult population is 29.4%.Prunus divaricata Ledeb.was one of resistant species and germplasm resource for Meloidogyne incognita because it resisted with the penetration and delayed the development of juvenile nematodes.The penetration inhibition is the major resistance mechanism.3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The current and future potential geographic distributions of H.postica were studied in this paper by using CLIMEX 3.0 and ArcGIS 9.3.The results showed that:at the current weather conditions,the suitable areas of alfalfa weevil (Hypera postica Gyllenhal) are Xinjiang,Gansu,Neimenggu,southeast Tibet,Sichuan and Yunnan in China.Based on projections of future climate in China showed that in 2020,it’s suitable area would expand to central and southern Tibet.The highly suitable area may southwards to 33 °N.The high suitable area would be increased from 29.38% to 35.1%,difference is 5.72%.Meanwhile,the non-suitable area would be reduced from 35.49% to 30.47%,difference is 5.02%.In order to prevent the further spread of the pest,the quarantine and monitor measures should be enhanced more in China.Keywords：Hypera postica;potential geographical distribution;CLIMEX;ArcGIS;grass4|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The objective of this experiment is to study the effect of simulated acid rain at different pH levels on physiological of Zephyranthes candida Herb.and the regulation function of Ca2+.With pot experiments,this paper studies the effect of simulated acid rain that included 5 different pH levels (pH 2.0,2.5,3.0,4.0 and 5.6) and the regulation function of Ca2+ on the organic matter metabolism ,and membrane damage,and antioxidant enzyme activity,Chl content ,and gas exchange parameters of Zephyranthes candida leaves.With the decrease of the pH,value of REC,MDA and SOD,increased gradually while value of soluble protein,soluble sugar,POD,Chl and Pn decreased gradually,Gs and Ci were firstly increased and then significantly reduced in pH2.0;when pH≤4.0,there was a significant correlation between Soluble sugar-pH meanwhile there were significant correlations between SOD-pH and Pn-pH.However,under the same acid rain coercion in the leaves pretreated with 10 mmol/L Ca(NO3)2,soluble protein,soluble sugar,POD,SOD and Chl,Pn increased of different level,and REC,MDA reduced significantly;each index did not change significantly with the pH lowing.Under simulated acid rain stress,pretreated with Ca2+could strengthen various physiological function of Zephyranthes candida Herb.,thus reduced the damage by acid rain.Experiments have found that the regulation effects of Ca2+ was more apparent in pH≤3.0.2|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A lab-scale tidal flow CW was studied to discuss the performance under the operation conditions.When the reflux ratio was 1∶1 and full of water to empty was 3 h∶3 h,the results showed that COD,BOD5 and ammonium removal efficiencies were 80.2%,79.0% and 61.8%,at loadings of 283.2,179.7 and 30.1 g/(m2·d) respectively,which were all higher than those of other types of CWs.The removal efficiency and removal loading of TP were 28.7% and 2.9 g/(m2·d).The oxygen supply could meet the need of oxygen in the tidal flow constructed wetland through the analysis of the oxygen transfers.Keywords：constructed wetland;tidal flow;removal loading;reoxygenation ability;nitrification2|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Our experiment was to investigate the effect of dietary supplementation with different type of fatty acid mixture on blood fatty acid profile and antioxidant capacity in dairy cows.Thirty six Chinese lactating Holstein dairy cows were randomly assigned to three treatments:1) control,2) long-chain fatty acid treatment (LCFA),and 3) short- and medium-chain fatty acid treatment (SMCFA).Experimental duration was 9 weeks and measurements were made during the last 8 weeks.Compared with control treatment, cows feed short- and medium-chain fatty acid significantly decreased the content of fatty acid with C＞16(P<0.05),and considerably increased the content of fatty acid with C≤16(P<0.05). Meanwhile, short- and medium-chain fatty acid significantly increased the ratio of saturated fatty acid and unsaturated fatty acid (P<0.05), and remarkably decreased the content of unsaturated fatty acid (P<0.05). On the other hand, there was no significantly effects on blood fatty acids were observed in LCFA treatment. Compared with control treatment, short- and medium-chain fatty acid tended to increase the activity of catalase and glutathione peroxidase, only glutathione peroxidase reached the significantly level (P<0.05).However,LCFA treatment significantly increased malondialdehyde content in serum (P<0.05).These results indicate that short- and medium-chain fatty acid would increase the antioxidant capacity of serum, and decreased the content of malondialdehyde in serum.Nevertheless,short- and medium-chain fatty acid would increase the total saturation of blood fatty acid.Keywords：dairy cows;fatty acid mixture;fatty acid;antioxidant capacity0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In order to determine the appropriate additive level of different zinc sources, help feeders make correct choice between zinc sulfate (ZnSO4) and zinc amino acid chelate (Zn-AA).Twelve 2.5-3.0 year old, 40-45 kg body weight Guanzhong dairy goats, with three site permanent fistulas in rumen, duodenum and ileum were selected and randomly assigned to two groups.The goats were feeding with non zinc additive dietary,zinc level were provided by infusion two form of zinc solution,ZnSO4 and Zn-AA at zinc levels of 0,20,40,60,80,100 and 200 mg/kg of RM(rumen mass),respectively.Samples of duodenum chyme, ileum chyme,dung and serum were collected discontinously to detect zinc content, and small intestinal and whole intestinal zinc digestive rate were calculated.The results showed that,1) Zn-AA had a higher digestion rate than ZnSO4 overall, small intestine is the main absorption part in dairy goats, but large intestine were manifested also had strong absorbency to the two zinc source. 2) Serum zinc level was not rise with the high digestion rate of Zn-AA, but follow zinc levels of ZnSO4 and Zn-AA respectively.3) Optimum ZnSO4 level in dairy goat was 40 mg/kg RM, its optional concentration range was 40-60 mg/kg of RM. Optimum Zn-AA level in dairy goat dietary was 60 mg/kg of RM, its optional concentration range was and 40-80 mg/kg of RM. It can be concluded that ZnSO4 and Zn-AA could be absorbed in small intestine as well as in large intestine,Zn-AA in general had a higher digestion rate and stronger tolerant than ZnSO4.Serum zinc level depends on the zinc source and levels due to different absorptive mechanism of two zinc forms.2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The purpose of this research was to investigate the antiendotoxin action of Pulsatillae Decoction (PD).PD was extracted and condensed into the decoction at the crude drug concentration of 1 g/mL.Mice in prevention groups were challenged with 9 mg/kg lipopolysaccharide (LPS) by peritoneal injection after the treated with PD at three different concentrations (0.1,0.5 and 1 g/mL) for 5 days,and differently,mice in treatment groups were challenged with 9 mg/kg LPS by peritoneal injection before treated with PD at three concentrations (0.1, 0.5 and 1 g/mL) for 5 days.The determined items and results were as follow:compared with LPS control group,the mortality were significantly decreased in the middle- and high-dose prevention groups and high-dose treatment group;the body weight were significantly increased in the high-dose treatment and prevention groups at 24 h;the WBC,HGB,MCH and PLT were significantly increased in the high-dose treatment group;the index of liver,spleen and intestinal were significantly decreased in the high-dose treatment group.It suggested PD had significant antiendotoxin effect.Keywords：Pulsatillae Decoction;mice;LPS;mortality;blood index;organ index0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To establish the animal model of infectious bursal disease and provide the data base and basic theories of this diseases,5 weeks old SPF chickens were infected by a IBDV strain BC6/85,histolpathological and Immunohistochemistrical methods were used to study the change of the pathological lesions and IBDV immunoreactive positive cells in bursa of fabricius from 2 to 432 h after infection.Results showed that IBDV and pathological lesion could be observed in the chamber and follicles of the bursa of fabricius in infected chickens and the IBDV detected after lesions appearing.Both of the pathological lesion and IBDV immunoreactive positive cells in bursa of fabricius increased at the beginning and then became different because of the positive cells disapeared with the disapearances of the bursal follicles.IBDV and bursal follicles still can be detected in the busal of infected chickens in the later period of infection.The study detail recorded the whole phases of the disease of the chickens infacted IBDV strain BC6/85 and installed the preliminary animal model.Then the form of marking was used to analyze the histological bursa lesions together with the density of IBDV immunoreactive positive cells,and searched mechanism of pathogenic.1|12|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The milk was added strain HCT which produce EPS to two conventional yogurt starters consisting of Lactobacillus Streptococcus thermophilus and Lactobacillus bulgaricus,and the non-HCT milk was used as control.The physicochemical,rheological and texture properties of yogurt were measured.The results displayed that compared with non-HCT contrast,adding 1% HCT strain to the mixed starter,the time of coagulation was reduced half an hour and the hardness,adhesiveness were promoted 0.23 N and 3.81 N;however,adding single starter exhibited lowed acid producing and prolonged fermentation time,also the hardness,adhesiveness were decreased 0.12 N and 1.0 N,but the cohesion was increased by 0.15.The results showed that adding HCT strain could significantly increase the viscosity of yogurt products,and enhance shear stress resistance,and also improve the color,taste and organizational status of yogurt;however,the effect on water holding capacity was not notable.Keywords：Lactobacillus paracasei;exopolysaccharide;yoghurt quality5|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The study was aimed at finding out the seed processing key parameters of combination of sieve on air-and-screen to improve the value of processed rice seed by increasing purity and germination percentage.The rice seeds of 18 mainly cultivated varieties of indica and japonica were collected in Yunnan province.On the basis of the seed size being measured,the key parameters were studied by utilizing the combination of processing sieve with different hole size at the LA-LS small type air-screen separation machine imported.The results showed that the average rice seed size of japonica is 1.40,and of indica is 1.33.It was found that the seed size,the combination of sieves with different hole size,the seed purity and the separation rate were linearly correlated.For Japonica rice finely separating,the hole size of the top sieve was 2.60 mm,the hole size range of bottom sieve was 1.70-1.90 mm.The optimal sieve hole ration of top and bottom sieve was 0.73.For indica rice seed finely separating,the top sieve hole size range was 2.25-2.60 mm,for the bottom sieve hole was 1.50-1.90 mm,and the optimal sieve hole ration of top and bottom sieve was 0.63.The separating technical parameter from this study was tested by industrial seed processing machine CIMBRIA DELTA SUPER 104 which can process 4 t/h of rice seeds.The test results proved the validity of key parameters of combination of sieve which can contribute to improve the quality and efficacy of seed processing and provide the important technical support for rice seed air-screen finely separation and standardized processing.Keywords：rice;seed;air-and-screen processing;seed purity;separation rate0|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on the field experiment of the 1SZ-460 vibration subsoiler,some critical mechanisms were redesigned to solve the problem such as bearing damage easily and beams deformation.So,an novel vibration subsoiler were designed to meet the market need.Firstly,simply-supported eccentric shaft type vibration generator mechanism was designed.Secondly,the vibration actuator component and the frame of machinery were optimized.Again,authors analyzed working performance and soil resistance of different shanks to improve the design of subsoiling shovel handle.Finally,a fertilizing system was added to reduce the numbers of machine works in field and soil compaction,and the product was enhanced.The consequence of the field experiment showed that compared with non-vibration subsoiler,the structure of 1SZ-460 vibration subsoiler were useful and rational which could reduce drawing resistance by 7%-17% and improve work efficiency by 11.1%-16.2%.The new machine is suitable for vibration deep loosening and fertilization work.Keywords：vibration subsoiler;vibration trajectory;soil deep loosening;fertilizing;shank0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The existence of faulty sensor nodes in wireless sensor networks (WSN) causes not only degradation of the network quality of service,but also a huge burden of the limited energy.Therefore,a method of accurate assessment about node failure status needs to be discussed.This paper adopts time series analysis and GA-BP neural network to establish a sensor nodes fault diagnosis system based on the combination of time series analysis and GA-BP neural network.With the aid of analyzing the sensor sample data and extracting model parameters as characteristic vectors,a net training process based on characteristic vector was used to build a GA-BP neural network to detect the sensor nodes faults and improve the diagnosis accuracy.The research result shows that the fault diagnosis method can effectively detect the sensor nodes failure type,the Euclidian Distance Sum of 15 test samples output vector and similar failure vector is 0.007 and the average recognition success rate is 100%.Keywords：sensor nodes;fault diagnosis;time series;BP neural network;GA0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：When the sensitivity of agricultural output to input resources and the rationality of resources’ inputs are discussed using the elasticity coefficient,it is necessary to integrate various outputs.Because in actual agricultural production system,the input resources and the outputs are variable given usually the input data of the specific resource and the corresponding output data might be hardly collected.In order to compare and discuss the relationship between the input of the specific resource and the output the weighted TOPSIS (Technique for Order Preference by Similarity to Ideal Solution) method,which has unambiguous geometric meaning and is simple and convenient,is used to integrate the various outputs.Using the elasticity coefficient of the specific resource and the TOPSIS method the states of agriculture resource utilization in Anhui province are discussed.The results indicate that the agricultural integrated output in whole province is highly sensitive to the inputs of the pesticide,the number of agriculture laborer and the effective irrigated area.The inputs of resources were reasonless in 2003,2005 and 2007,but relatively reasonable in 2004,2006 and 2008.In the mountainous area of the South Anhui province the inputs of resources were reasonless in 2002,2003,2005 and 2007,but relatively reasonable in 2001,2004,2006 and 2008.The practice of agriculture production in Anhui province indicated that the integrated output may be also increased by decreasing the inputs of resources.Keywords：agricultural economy;input-output;elasticity coefficient;weighted TOPSIS method0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The ecological footprint method is an assessment method of the human appropriation of ecological capacity,for measuring the ecological imperative of sustainability.This research applied the constant global yield method to calculate and analyze the ecological footprint of Gansu province from 1990 to 2007,also analyze development trend of per capita ecological footprint and ecological capacity.The result showed that during 1990 to 2007,the value of per 10 thousand YUAN GDP footprint had a downtrend of velocity deceleration,and footprint diversity varied gradually;the growth trend of ecological deficit will be enlargement from 2007 to 2017 and per capital ecological deficit will be 2.733 hm2.Thus,to form a sustainable development pattern for Gansu,as indicated by the conclusions,the productivity and efficiency of resource utilization should be pushed up to a higher level to enhance the ecological capacity.Keywords：Gansu Province;ecological footprint;ecological capacity;sustainable development5|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
