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- Abstract：Recently， sugarcane virus disease has frequently spread and erupted， showing an increasing tendency of prevalence. This study systematically summarized the current status and pathogenic mechanisms of sugarcane virus infections. Literatures published from 2015 to 2025 in CNKI and Web of Science databases were retrieved using ‘sugarcane’ and ‘virus’ as topics， and a total 6 Chinese and 107 English publications were reviewed. This review summarizes viral diseases affecting sugarcane in China over the last decade and analyzes the classification， virion characteristics， genome structure， symptoms， transmission vectors， and pathogenic mechanisms of different viruses and explores the potential of biotechnology， including transgenic technology， RNA interference （RNAi）， gene editing， new medicine development， and intelligent breeding in developing virus-resistant sugarcane varieties and diseases control strategies. The results indicate that： 1） A diverse range of sugarcane viruses and severe co-infections are coupled with limited virus resistance in current Chinese sugarcane varieties； 2） The occurrence of sugarcane viral diseases is related to variety resistance， environmental conditions， and virus transmission routes； 3） Biotechnological strategies， including transgenics， RNAi， gene editing， and anti-viral medicine development offer promising avenues for enhancing viral disease resistance in sugarcane. In conclusion， effective sugarcane viral disease management requires a comprehensive approach integrating traditional methods， modern biotechnology， and intelligent breeding strategies to select superior cultivated sugarcane for disease resistance， high sugar content， and high production.Keywords：sugarcane viral disease;virus classification;disease management;control strategies190|84|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To optimize the fermentation conditions and extraction methods for double-stranded RNA （dsRNA） in a prokaryotic expression system and evaluate its potential for controlling rice sheath blight （Rhizoctonia solani）， a recombinant hairpin expression vector targeting the RsDCL1/2 genes of R. solani was constructed and transformed into Escherichia coli HT115（DE3）. Six fermentation parameters （pH， IPTG concentration， induction temperature， agitation speed， duration， and NaCl concentration） were systematically optimized. The results showed that the highest dsRNA yield （1.32-fold increase over pre-optimization levels） was achieved under the following conditions： pH 7， 0.4 mmol/L IPTG， 33 °C， 160 r/min， 7.5 g/L NaCl， and 12 h induction. Comparative analysis of four extraction methods （Trizol， RNAswift， 75% ethanol fixation， and PB buffer lysis） demonstrated that PB buffer lysis yielded 3.89 times more dsRNA than 75% ethanol fixation while maintaining superior purity. In greenhouse trials， the appliction of PB-extracted dsRNA foliar significantly reduced the lesion area on rice leaves， confirming the potential dsRNA as a biopesticide in the prevention and control of rice sheath blight. Collectively， the integrated dsRNA production system combining optimized fermentation and PB buffer lysis not only enhanced dsRNA yield but also exhibited promising field-applicable potential for sustainable management of rice sheath blight.Keywords：dsRNA;optimization of fermentation conditions;dsRNA extraction methods;rice sheath blight103|30|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：In order to screen microbial resources that can effectively prevent and control cucumber root knot nematode disease， excellent strains were selected through in vitro nematicidal assays. The strains were identified based on their morphological characteristics， physiological and biochemical properties， and determined by molecular biology methods. The effects on nematode egg hatching rate and cucumber seed germination rate were determined， and the control effects of the strains on cucumber root knot nematode disease were further determined through pot and field experiments. The results showed that strain Sneb2562 displayed high toxicity activity against second-stage juvenile （J2） of Meloidogyne incognita was screened from 52 bacterial strains preserved in this study. After 24 h of treatment， the mortality rate of M. incognita J2 exposed to Sneb2562 reached 79.25%. Based on morphological characteristics， physiological and biochemical properties， and molecular biology methods， the strain Sneb2562 was identified as Bacillus velezensis. B. velezensis Sneb2562 treatment significantly inhibited the hatching of nematode eggs， exhibited the capabilities to produce protease， phosphatase， amylase， and solubilize phosphate. The germination rate of cucumber seeds treated with B. velezensis Sneb2562 increased by 4.00% compared with the LB control （P>0.05）. The results of the pot experiment showed that Sneb2562 significantly reduced the number of root galls on cucumber plants after nematode infestation， with a root gall reduction rate and control effect of 50.27% and 52.00%， respectively. Additionally， this strain significantly increased the height and fresh weight of cucumber plants， with the plant height and fresh weight increasing by 16.05% and 63.68% compared to the LB control， respectively （P<0.05）. In addition， the field trial results in the 1st field experiment of 2024 showed that the control effect of strain Sneb2562 on cucumber root knot nematode disease was 42.03%. The fresh weight of cucumber plants treated with this strain increased significantly by 4.01% compared to LB （P<0.05）. The experimental results in the 2nd field experiment of 2024 showed that strain Sneb2562 had a 53.73% control effect on cucumber root knot nematode disease， and the plant height and fresh weight of cucumber plants treated with it were significantly higher than LB， increasing by 25.18 cm and 29.41 g， respectively （P<0.05）. In summary， B. velezensis Sneb2562 can effectively control cucumber root knot nematode disease， and has great potential for development and application， and can be used as a new potential biocontrol resource for the biological control of this disease.Keywords：Meloidogyne incognita;Bacillus spp.;identification;biocontrol effect78|20|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To construct a combination of biocontrol bacteria with efficient prevention and control effects on apple replanting disease， the antagonistic bacterium ‘H166’ and growth-promoting bacterium ‘C35’ obtained from previous screening were identified for affinity and a combination of bacteria was constructed. Through indoor experiments， the antibacterial activity of the combination bacteria against four pathogenic fungi， namely Fusarium oxysporum， F. solani， F. proliferatum， and F. moniliforme，and the effects of the fermentation broth， sterile filtrate， and extracellular metabolites of the combination bacteria on the spore germination of F. oxysporum and the mycelial morphology of the four pathogenic F. oxysporum strains mentioned above， as well as their antibacterial effects on other plant pathogens were determined pot and field experiments were adopted to investigate the prevention and control effect of combined bacteria on apple replanting disease. The results showed that：The antagonistic bacterium ‘H166’ （Bacillus amyloliquefaciens） exhibited good affinity with the growth-promoting bacterium ‘C35’（Pseudomonas aeruginosa）. The two bacterial strains were combined as ‘H166+C35’ at a 1：1 volume ratio. The antibacterial rates of the sterile filtrate of the combination bacteria against F. oxysporum， F. solani and F. proliferatum were 58.33%， 36.88%， and 44.25%， respectively. Electron microscopy revealed that the combination of bacteria caused hyphal branching， breakage and enlargement of four pathogenic fungi. The fermentation broth， sterile filtrate， and extracellular metabolites of the combination bacteria can significantly inhibit the spore germination of the Fusarium oxysporum， with germination rates of 52.66%， 19.75%， and 12.88%， respectively， compared to sterile water （ 98.44%）. The bacterial combination showed stronger inhibitory effects on Botryosphaeria dothidea and Alternaria alternata than single bacterium， and the inhibition rates are all over 71%. In the pot experiment， the height， root length， root fresh weight and number of lateral roots of the eight-edge crabapple（Malus robusta Rehd.） treated with the combination bacteria increased by 20.07%， 47.23%， 64.33%， and 68.54%， respectively， compared to water treatment. The protective and therapeutic effects reached 74.82% and 68.15%， respectively. Field experiments discovered that the death rate of seedlings treated with the combination bacteria is 0%， which was significantly lower than that of a single strain （‘H166’ 10% and ‘C35’ 30%） and the water treatment （50%）. In summary， the ‘H166+C35’ combination reduces apple replanting disease by disrupting fungal hyphae， inhibiting spore germination， and enhancing plant resistance， demonstrating potential for production control.Keywords：apple replant disease;Fusarium spp.;biocontrol bacteria;combined bacteria;control efficacy46|21|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：In order to obtain biocontrol bacteria with strong antagonistic activity against Erwinia amylovora， analyze its biocontrol characteristics and evaluate its biocontrol effect on pear fire blight， different forms of bacteria were isolated and purified from pear flowers， young fruits， and tender shoots collected from the orchards with pear fire blight in Korla City， Xinjiang. The plate confrontation method was used to screen strains with strong antagonistic activity against E. amylovora. Based on the morphology， physiological and biochemical characteristics， and 16S rDNA sequence， its classification status was clarified. The suitable temperature for bacteria cultivation， acid production ability， and stability of secondary antibacterial substances were determined. The antibacterial activity of its secondary metabolites was compared with three bactericides for E. amylovora. The morphological and structural changes in E. amylovora cells treated with secondary metabolites of the strain identified was analyzed using transmission electron microscopy. Furthermore， the plate antibacterial test and greenhouse biocontrol test were used to evaluate the efficacy of the biocontrol strain. The results showed that： 1） Leuconostoc mesenteroides ‘WZ-44’ was isolated and identified from the surface of young pear fruits， which displayed strong antagonistic activities against E. amylovora， E. pyrifoliae， Pseudomonas syringae pv. lachrymans， and Pectobacterium carotovorum， and the antagonistic circle diameter （d） was within range 17.7-24.7 mm； 2） The ‘WZ-44’ strain has a wide suitable growth temperature range （15-35 ℃） and strong acid production ability. Its secondary metabolites also has strong antibacterial activity against E. amylovora （its antagonistic circle diameter （d） is 26.0±0.4 mm）， which is equivalent to 40 μg/mL kasugamycin hydrochloride （25.4±0.5 mm） and significantly （P<0.05） better than 100 μg/mL benziothiazolinonc （13.9±0.9 mm） and 500 μg/mL zine thiazole （7.4±0.8 mm）； 3） The antibacterial activity ‘WZ-44’ secondary metabolites is the strongest at pH 3.0， and it has good tolerance to high temperature， protease， and ultraviolet irradiation. It could destroy the integrity of pathogenic bacterial cell membranes， leading to extravasation of cell contents， cell deformation， and even collapse and death； 4） The‘WZ-44’ strain has good compatibility with kasugamycin hydrochloride. The ‘WZ-44 ’biocontrol agent has a （76.1±9.6）% preventive effect for pear fire blight during the flowering period. In summary， ‘WZ-44’ has strong antagonistic activity against E. amylovora and stable secondary antibacterial substances. It is easy to be fermented and cultivated， and has good biocontrol effect. It can be used as a new type of biocontrol bacterium to prevent and control pear fire blight.Keywords：pear fire blight;Leuconostoc mesenteroides;biological control;secondary metabolites38|18|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：Lobed leaf is an important feature for plant to adapt environment， and the mining of watermelon lobe leaf-related genes is of great significance for watermelon breeding， genetic improvement and molecular regulatory mechanisms of leaf margin morphogenesis. Firstly， six generations of genetic populations （P1， P2， F1， F2， BC1P1 and BC1P2） were constructed by crossing the watermelon lobed leaf inbred line SW246 （P1） with the entire leaf inbred line SW247 （P2） as the parental parents. Later， gene localization was carried out by using BSA-seq and association analysis was conducted in combination with RNA-seq data from transcriptome sequencing， To excavate the key regulatory genes of leaf lobed. The key regulatory genes were analyzed by BSAseq and RNA-seq. The results of genetic analysis showed that in the progeny population， the watermelon leaves of F1 and BC1P1 populations were all lobed leaves. The segregation ratio of lobed and entire leaves in the F2 population conformed to the Mendelian segregation ratio of 3∶1. In contrast， the segregation ratio of lobed and entire leaves in the BC1P2 population conformed to the theoretical ratio of 1∶1， which indicated that the traits of lobed and entire leaves conformed to the single-gene dominant mode of inheritance. The lobed was dominant to entire leaves. BSA-seq analysis revealed that the SNPs and InDels association intersections were located within 23 164 320 to 24 976 967 bp of chromosome 4， with a length of 1.81 Mb， containing 171 candidate genes. Among them， there were 22 SNPs with non-synonymous mutations between parents and 16 SNPs with non-synonymous mutations between mixed pools； 3 InDels with shifted code mutations between parents and 4 InDels with shifted code mutations between mixed pools. Combining BSA-seq and RNA-seq analysis， it was revealed that a total of 26 genes were significantly associated. In addition， the bioinformatics analysis， transcription factor prediction， and gene annotation of the four candidate genes and 26 significantly related genes obtained from the combined BSA-seq and RNA-seq analyses resulted in the speculative discovery of one gene encoding a PPR protein （Cla97C04G076940）， which is related to HD-Zip， TCP， LOB， E2F-DP， TIFY， and other transcription factors， and another gene is related to IAA-amino acid hydrolysis and E3 ubiquitin ligase， which may play a key role in the regulation of leaf margin traits. This study lays the foundation for further fine localization of watermelon leaf margin regulation genes.Keywords：watermelon;lobed leaf;BSA-seq;RNA-seq;candidate genes43|17|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
Effects of different fruit bags on the quality of protected peach and their comprehensive evaluation
Abstract：To explore the influence of different fruit bags on the fruit quality of protected peach， two varieties of protected peach， ‘C26-7-17’ and ‘Ruipan 25’， were used as test materials， with no bagging as the control. Four treatments， including yellow bag， white bag， and outer yellow and inner black bag， were set up to measure the appearance and internal quality of the fruit. Correlation analysis and principal component analysis were conducted on the measured indicators. A comprehensive evaluation was conducted on the application effects of the three types of fruit bags. The results showed that： 1） In terms of appearance quality， the three types of fruit bags had the same effect on the skin coloring of the two varieties. Under the treatment of yellow bag and outer yellow inner black bag， the brightness of the fruit peels of the two varieties increased， the coloration became yellower， and the redness decreased. The fruit weight of ‘C26-7-17’ significantly increased when treated with a yellow outer layer and black inner bag （P<0.05）， while that of ‘Ruipan 25’ significantly decreased （P<0.05）. The bagging reduced the hardness of the bark of both varieties， with the ‘C26-7-17’ being the most obvious. The fruit weight was significantly positively correlated with the longitudinal diameter， and b* was significantly positively correlated with the longitudinal diameter. 2） In terms of internal quality， bagging had no significant effect on the soluble solids content of the two varieties. The glucose content of ‘C26-7-17’ was significantly reduced under the yellow bag and white bag treatments （P<0.05）， while that of ‘Ruipan 25’ was significantly increased （P<0.05）. All three fruit bags reduced the malic acid and citric acid contents of ‘C26-7-17’ and increased the tartaric acid content. Bagging increased the malic acid content of ‘Ruipan 25’， yellow bags and white bags increased the citric acid content， and white bags and those with yellow outside and black inside reduced the tartaric acid content. There was an extremely significant positive correlation between the sucrose content and the glucose content （P<0.01）， while the citric acid content and tartaric acid content were both extremely significant negative correlations with the sucrose content and the glucose content （P<0.01）. Moreover， the impact of bagging on quality varies by variety. 3） The most suitable fruit bags for the comprehensive evaluation of the ‘C26-7-17’ and ‘Ruipan 25’ were outer yellow and inner black bags and yellow bags， respectively. The research results provide reference for the application of fruit bags in protected peach production in the northern regions.Keywords：peach;facility cultivation;fruit bag;fruit quality;comprehensive evaluation43|42|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06- Abstract：To study the mechanism of humic acid-based biodegradable liquid film （BLF） affecting the freezing tolerance of grapevines， the dormant vine variety ‘Cabernet Sauvignon’ was taken as material， vines sprayed with BLF as the treatment group， and grapevines sprayed with water as the control group （CK）. Differential thermal analysis was conducted to evaluate the effect of BLF treatment on the freezing tolerance of grapevines. Transcriptome sequencing was performed on grapevines under different low-temperature stress conditions， the key metabolic pathways were identified by GO enrichment and KEGG enrichment analyses， and the activities of enzymes related to carbohydrate metabolism and the content of saccharides were also determined. The results showed that： 1）BLF treatment reduced the supercooling and freezing temperatures of grape winter buds. 2） Under the condition of -10 °C stress for 6 h， the CK and BLF groups had the highest number of differentially expressed genes （DEGs）， and the co-expressed DEGs were mainly related to the pathways of carbohydrate metabolism， secondary metabolite synthesis， and phytohormone signaling. 3） A total of 23 significantly different pathways were enriched in DEGs from CK and BLF-treated plants （P≤0.05）， including signal transduction， lipid metabolism， carbohydrate metabolism， biosynthesis of other secondary metabolites， and amino acid metabolism. Among them， carbohydrate metabolism-related pathways showed significant （P≤0.05） or highly significant （P≤0.01） differences under most stress conditions. 4） BLF treatment up-regulated the expression of genes encoding cellulase， pectinase， and invertase， and down-regulated the expression of genes encoding β-amylase， glyceraldehyde-3-phosphate dehydrogenase， and ethanol dehydrogenase. 5） Further analysis of enzyme activities and sugar contents showed that BLF treatment significantly increased the activities of β-glucosidase， total pectinase and invertase （P≤0.05）， significantly increased the starch， soluble sugar and reducing sugar contents （P≤0.05）， significantly decreased the activity of β-amylase and sucrose content of the branches （P≤0.05）. In conclusion， BLF treatment improved the freezing tolerance of grapevine vines， through the changes in carbohydrate metabolic pathways.Keywords：grapevine;humic acid;low temperature stress;transcriptome sequencing;carbohydrate metabolism48|25|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：This study investigated the degradation dynamics of Miscanthus lutarioriparius derived biochar and its rhizome biomass in soil. The aim was to enable the scientific utilization of M. lutarioriparius resources for carbon reduction and emission mitigation. M. lutarioriparius derived biochar and its rhizome biomass were added to the paddy soil for an indoor incubation experiment. The carbon content of M. lutarioriparius derived biochar and its rhizome biomass was measured at different time intervals under varying incubation conditions to characterize their degradation. Degradation characteristics were analyzed by fitting the data with kinetic equations.Resultsdemonstrated that incubation time exerted a greater influence on carbon degradation than incubation conditions for both biochar and rhizome biomass. Specifically， rhizome biomass exhibited rapid early-stage degradation under warm-flooded conditions but accelerated late-stage degradation under low-temperature controlled-water conditions. Biochar demonstrated higher carbon retention rates at elevated application levels. For M. lutarioriparius biochar under simulated paddy soil conditions， the degradation half-life increased with higher application rates. The longest half-life （44.42 years） occurred at the biochar application rate of 18.0 t/hm⁻². The degradation half-life of M. lutarioriparius rhizome biomass was determined to be 2.67 years under simulated warm-flooded soil conditions and 1.45 years under simulated low-temperature， water-controlled conditions. The comprehensive half-life of rhizome under natural field conditions was calculated as 1.76 years. In conclusion， this study establishes a foundation for carbon footprint accounting of the “M. lutarioriparius -Biochar” production and utilization model in the Dongting Lake region. It also provides critical references for optimizing regional carbon sink management strategies.Keywords：Miscanthus lutarioriparius;biomass;biochar;carbon stability;degradation half-life44|27|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：In order to generate a nanobody （Nb） that specifically recognizes chicken interleukin-18 （chIL-18）， this study constructed a recombinant plasmid expressing chIL-18 protein and produced chIL-18 protein. The chIL-18 protein was used to immunize alpacas， and the variable regions of the heavy chain of the antibody genes from the peripheral blood lymphocytes of the immunized alpacas were amplified to construct a chIL-18 Nb phage display library. The library was enriched and selected using chIL-18 protein as the target antigen， and the sequences of the recombinant phage Nb that reacted positively with chIL-18 protein were cloned and expressed on the vector. Furthermore， after conducting biological activity tests on chIL-18 Nb， a preliminary application evaluation was carried out on the chIL-18 Nb with the best biological activity. The results were as follows： 1） In this study， a chIL-18 Nb phage display library with a capacity of 6×108 PFU/mL was successfully constructed； 2） Five chIL-18 Nbs with good reactivity， specificity and binding activity were obtained， among which Nb1 had the best biological activity and met the requirements for antibody application in western blot and IFA tests. This study was the first to obtain the Nb specifically reacted with chIL-18 protein， which laid a foundation for subsequent establishment of quantitative detection methods for chIL-18 and the research about the biological functions of chIL-18.Keywords：chicken interleukin-18;nanobody;phage display;characterization22|16|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To analyze the ability of busulfan injected into hatching eggs to ablate chicken endogenous germ cells and its effect on hatching performance， and to determine the optimal dosage protocol， this study prepared busulfan sustained-release formulations using sesame oil. The agents were injected into the yolk of hatching eggs at 3 hours of incubation at final busulfan doses of 0 （blank control group without injection and test control group injected with reagents without busulfan）， 50， 75， and 100 μg per egg， respectively. The ablation of endogenous germ cells in chicken embryos and hatching performance were compared among groups. The results showed that： 1） Busulfan injection significantly reduced the number of primordial germ cells （PGCs） in the gonads at 7 embryonic days （E7） （P<0.05）， demonstrating good ablation ability. There was no significant difference in the number of PGCs between the 75 and 100 μg groups （P>0.05）， but both groups contained significantly fewer PGCs compared to the control and the 50 μg group （P<0.05）.2） In terms of hatching performance， the 75 μg treatment group demonstrated significantly lower embryonic mortality（43.2%） compared to the 100 μg group （71.6%）. 3） Dissection of chicks and dead embryos found that the gonads of each test group were relatively small， and part of the digestive tract （muscular stomach） was damaged to varying degrees. In conclusion， the busulfan sustained-release agent can effectively eliminated endogenous germ cells and achieve successful hatching. The dose of 75 μg/egg is the optimal concentration for preparing transplantation recipients. However， further research is required to significantly reduce embryonic mortality rates.Keywords：busulfan;sterile recipient;germline transmission rate;toxic effect33|16|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To investigate the causes of diarrhea and even death among dairy cows in a farm in Beijing，this study isolated and purified a bacterial strain from the spleen of a diarrheic calf in Tongzhou District， Beijing. The strain was subjected to 16S rRNA gene sequencing， antimicrobial susceptibility testing， virulence gene screening， and whole-genome sequencing （WGS） to analyze its virulence， resistance profile， and genomic features. The results showed that： 1） The isolated strain was identified as Salmonella enterica subsp. enterica serovar Dublin（S.Dublin） and designated SDWW24. 2） Antimicrobial susceptibility testing revealed that isolate strain SDWW24 exhibited multidrug resistance （MDR） to eight antibiotic classes， including cephalosporins， macrolides， and aminoglycosides. 3） PCR method screening detected 12 common Salmonella virulence genes with a 100% detection rate. 4） The whole genome length of the isolated strainSDWW24 was 4 736 488 bp， carrying a plasmid with a size of 59 372 bp. A total of 4 618 genes were predicted， including 1 082 virulence-associated genes and 168 antimicrobial resistance （AMR） genes， with high concordance between genotypic predictions and phenotypic resistance patterns. In conclusion， this study isolated and identificated a strain of S.Dublin from cattle， and analyzed its basic biological properties and whole genome characterization， which provides a reference basis for the follow-up research of S.Dublin.Keywords：Salmonella Dublin;whole-genome sequencing;virulence gene;antibiotic resistance;functional analysis28|22|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To systematically elucidate the evolutionary patterns， molecular architecture， and functional characteristics of the Solute Carrier Family 38（SLC38） gene family in cattle， this study employed bioinformatics approaches analysis to investigate genomic features of SLC38A family members， combined with RT-qPCR to investigate their dynamic expression profiles in adipose tissues and during adipocyte differentiation. The results showed that： 1） Members of the SLC38A gene family exhibited significant heterogeneity in both isoelectric points（pI） and molecular weights（MW）. 2） Chromosomal localization revealed distinct genomic distributions in cattle， while phylogenetic analysis and conserved domain characterization demonstrated evolutionary diversification of motif-domain combinations. Collinearity analysis further elucidated conserved syntenic relationships between bovine SLC38A genes and other species. 3） Expression profiling identified SLC38A1/A2/A10 as predominant isoforms in bovine adipose tissues， with significantly higher expression levels in Angus cattle compared to Simmental cattle. During adipocyte differentiation， their expression was transiently downregulated on day 2， followed by a significant upregulation in the late stages of differentiation. Collectively， this work delineates the evolutionary conservation and functional divergence of SLC38A family across species， uncovering their pivotal regulatory roles in lipid metabolism and energy homeostasis. These findings establish a theoretical framework for further deciphering the molecular mechanisms underlying lipid metabolic regulation mediated by this gene family.Keywords：SLC38A gene family;bioinformatics analysis;expression pattern32|17|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To address the time-consuming and labor-intensive issues associated with traditional sheep body measurement methods， this study employed an integrated approach combining iteratively reweighted least squares（IRLS）， k-means algorithm， Gaussian mapping and Gaussian kernel mean shift clustering algorithm for analyzing sheep body dimension measurement based on 3D point cloud. The results showed that： 1） The normal estimation method of the iterative reweighted least squares method demonstrated strong noise immunity and yielded more accurate results. 2） The mean shift clustering algorithm based on the Gaussian kernel can accurately identify and separate the sheep body area data from the point clouds， effectively avoiding the over-segmentation or under-segmentation of other methods precalent in alternative methods. 3） Body measurement points were selected from the sheep point cloud to calculate four morphometric parameters such as body length， body height， chest depth， and rump height. The maximum relative error of the four body size parameters was 1.72%. In summary， the proposed 3D point cloud-based approach enables accurate extraction of sheep body point clouds and non-contact morphometric measurement through strategically selected anatomical landmarks. This methodolody provides a reliable technical references for both body dimension quantification and live weight estimation management.Keywords：sheep;body measurement;point cloud segmentation;computer vision36|21|1<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To screen candidate transporters involved in cordycepin efflux within Cordyceps militaris， the study employed bioinformatics approaches to analyze ATP-binding cassette （ABC） transporters across its genome. By integrating transcriptomic data， previously validated findings， and molecular docking analyses， transporters correlated with cordycepin accumulation were identified. The results showed that： 1） A total of 70 ABC transporters were identified at the genomic level， distributed in the ABCA （3）， ABCB （12）， ABCC （37）， ABCD （2）， ABCE （1）， ABCF （5）， ABCG （8）， ABCI （1） and YDR061W （1） subfamilies. 2） Integrating transcriptome data of C. militaris from three projects， 96 membrane proteins significantly positively correlated with cordycepin accumulation were screened. These proteins were compared to the whole genome proteins of Saccharomyces cerevisiae， Aspergillus nidulans， and Aspergillus oryzae， and shared proteins with a similarity greater than 30% were screened. Across the entire genome of C. militaris， proteins homologous to ENT1/ENT2 of Homo sapies， CaCNT of Candida albicans， and TbAT of Trypanosoma brucei were investigated and combined with the selected shared proteins to obtain 34 candidate transporter proteins. 3） By constructing a three-dimensional protein structure model and performing molecular docking analyses， the binding energies of all transporters to cordycepin were less than -5 kJ/mol. Subcellular localization predictions showed that these proteins were mainly distributed in the plasma membrane （27）， vacuole （3）， endoplasmic reticulum （2）， peroxisome （1）， and mitochondrion （1）. In conclusion， through multi-strategy bioinformatics analysis， this study identified 70 ABC transporters in the whole genome of C. militaris CM01 and screened 34 candidate transporters potentially involved in cordycepin efflux in C. militaris CM01， laying the groundwork for future research on cordycepin transport mechanisms and metabolic reprogramming.Keywords：Cordyceps militaris;cordycepin;transporter;bioinformatics;molecular docking24|14|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：This study aimed to investigate the combined effects of straw returning coupled with different nitrogen fertilization strategies on the phosphorus （P） and potassium （K） utilization efficiencies of the direct-seeded cotton after barley and the soil P and K apparent balance. A two-factor split-plot design was employed. The main plots were two different straw returning rates： no straw return （S0） and full straw return （S1）. The subplots were four different nitrogen fertilization strategies， which were no nitrogen fertilizer （N0）， urea （U）， controlled-release nitrogen （CRN）， and urea+CRN （UC）. The impacts of their different combinations on the P and K accumulation and utilization efficiency of direct-seeded cotton， the soil P and K apparent balance and the soil available P and K contents were investigated. The results showed that： 1） Compared to S0， the P and K accumulation of shoot and reproductive organs under S1 significantly increased after the peak blooming stage and boll-opening stage. Under nitrogen fertilization application， the treatment CRN had lower P and K accumulation at the peak squaring stage but higher values at the boll-opening stage， whereas the treatment UC exhibited consistently higher P and K accumulation across different growth stages. The direct-seeded cotton under the treatment combinations of S1CRN and S1UC exhibited higher P and K accumulation at the boll-opening stage. Compared to S1U， the two-year average K accumulation of cotton plants at the boll-opening stage in S1CRN and S1UC treatment combinations increased by 12.9% and 10.4% in shoot， respectively， and increased by 1.9% and 3.6% in reproductive organs. 2） The cotton plant under the S1UC combination had higher yields than other combinations. Compared to S0， the utilization efficiency of P and K of the cotton plant showed no significant change under S1. The utilization efficiencies of P and K of the cotton plant were higher in the UC treatment but lower in the CRN treatment under nitrogen fertilizer application. 3）The soil P was deficient under S0， while it was deficient in 2021 and surplus in 2022 under S1. The soil K was deficient under S0 and surplus under S1. The surplus of soil P and K was greater in the U treatment under nitrogen fertilizer application. The soil P and K was just in apparent balance for the treatment combination of S1UC. 4）The treatment combinations of S0UC and S1UC had lower soil available P and K content. In conclusion， the straw returning of 6 000 kg/hm2 combined with UC of pure N 180 kg /hm2） facilitated the utilization of soil available nutrients， thereby improving the P and K accumulation and utilization efficiency of direct-seeded cotton after barley in coastal saline land， which achieved the synergy of high yield and the apparent balance of soil P and K.Keywords：direct-seeded cotton;straw returning;N fertilizer strategy;P and K utilization efficiency;P and K balance46|17|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To investigate the optimal turning frequency for efficient biodrying-aerobic fermentation of kitchen waste， this study established three treatments using kitchen waste and sawdust as substrates， which were no turning （CK）， turning every 3 d （B1）， and turning every 7 d （B2）. The effects of different turning frequencies on temperature increase， moisture reduction， organic matter degradation， and bacterial community structure succession during fermentation were systematically investigated by 16S rRNA amplicon sequencing and PICRUSt2 functional prediction analysis. The results indicated that： 1） B1 achieved the fastest temperature rise and highest peak temperature （The time to reach the mesophilic stage of B1 was one day earlier than that of CK， and its maximum temperature is 9.98% and 27.11% higher than that of CK and B2， respectively）. The thermophilic stage duration of B1 was longer， which was 4 d and 1 d longer than that of CK and B2， respectively）. 2） Throughout the biodrying process， the seed germination index （GI）， and at the end of biodrying， water removal rate （68.99%） and organic matter degradation rate （24.91%） were the highest in the B1 treatment. 3） The diversity and evenness of both the bacterial community structures in treatments B1 and B2 were higher than those in CK. Moreover， the bacterial network in B1 was more closely connected， and the bacterial community activity was higher during the mesophilic stage， especially on the 9th day. 4） Correlation analysis between bacterial community structure and environmental factors identified Symbiobacterium， Bacillus， Thermobifida and Pusillimonas as key taxa significantly correlated with temperature increase， moisture removal and maturation efficiency enhancement. These genera are potential candidates for developing directionally screened strains and compound microbial agents to enhance biodrying-aerobic fermentation. In conclusion， a turning frequency of once every 3 d is the optimal choice， which can not only improve the maturity of the products from the biodrying-aerobic fermentation of kitchen waste， but also optimize the structure of the bacterial community.Keywords：kitchen waste;auxiliary heat strengthening;biodrying-aerobic fermentation;turning frequency;bacterial community structure;co-occurrence network analysis;function prediction45|18|1<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To investigate the effect of the aeration rate on the maturity and humification during co-composting of food waste and garden waste， three treatments were set up. The aeration rates were 0.2， 0.4， and 0.6 L/（kg·min）， respectively， denoted as AR0.2， AR0.4， and AR0.6. A total of 15 days of aerobic composting were conducted. The maturity of compost was characterized using electrical conductivity （EC） and germination index （GI）， and its humification was assessed through indicators such as humic acid （HA） and the ratio of humic acid to fulvic acid. High-throughput sequencing technology was employed to investigate the succession patterns of bacterial communities during composting. Furthermore， correlation analyses were performed to determine the relationships between bacterial communities and humic acid precursors， humification indicators， and maturity parameters. The results indicate that： 1） The aeration rate of 0.4 L/（kg·min）was beneficial for the decrease of EC and the increase of GI， and thereby was beneficial for the maturation of compost. At the end of composting， the GI of AR0.4 was 85.31%， which was 10.20 and 4.49 percentage higher than those of AR0.2 （75.11%） and AR0.6 （80.82%）， respectively. 2） The aeration rate of 0.4 L/（kg·min） was beneficial for the convertion of reducing sugars， amino acids， and polyphenols to HA， and also for the humification of compost. At the end of composting， the HA values of the three treatments were 10.57， 17.04， and 13.96 mg/g， respectively， and the ratios of humic acid to fulvic acid were 0.88， 1.52， and 1.06， respectively. 3） The results of correlation analysis showed that the aeration rate of 0.4 L/（kg·min）inhibited the growth of Weissella and Lactobacillus， while promoted the growth of Saccharomonosora， Thermoactinomyces， Aerococcus， Aeribacillus， Bacillus， and Geobacillus. It was beneficial for the maturation and humification of compost. In summary， it is recommended to use the aeration rate of 0.4 L/（kg·min）during co-composting food and garden waste can facilitate the maturity and humification of compost.Keywords：maturity;humic acid;humic acid precursor;carbon nitrogen conversion;dominant microorganisms36|17|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：This study aims to address the behavioral logic and practical challenges in the adoption of whole-process manure resource utilization technologies in large-scale dairy farms. Multi-case analysis approach is adopted reveals the differences and influencing factors in technology application across farms of different scales and proposes optimization strategies is proposed to promote the green， efficient， and sustainable development of animal husbandry. The results found that： According to the resource endowment and cost conditions， different scale farms can significantly improve the efficiency of manure resource utilization and increase the farming income by optimising the combination of end-of-pipe management technologies for manure resource utilization， gradually perfecting the system of source reduction and process control technologies， and formulating the scientific technology application and management methods； In terms of the effect of manure resource utilization， the energy saving and efficiency increase of manure resource utilization is significantly higher in small-scale and medium-scale farms than in large-scale and ultra-large-scale farms. In terms of the effect of utilization of manure resources， small-scale， medium-scale farms utilize manure resources significantly more than large-scale and super-large-scale farms， and cooperation between large-scale farms and socialised service organisations is an important way to carry out the utilization of manure resources. Due to the imperfection in the whole process of manure resource utilization technology system， the main body of the farming community does not have sufficient knowledge of the value of the whole process of manure resource utilization technology， resulting in an imperfect system of technology application， and the relevant incentive and punishment mechanisms and social service organisations are still unsound. Therefore， we should avoid the blind copying and promotion of the technology model of manure resource utilization， choose the technology model adapted to different scales according to local conditions， promote the synergistic development of the main body of farming， the government and social service organisations， and improve the resilience and sustainability of the industrial system。Keywords：total process manure resource utilization;technology adoption logic;manure resource utilizsation model;typical case;practical dilemmas40|23|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To address the prominent contradiction between cultivated land damage and protection in coal mining subsidence areas， this study constructs an ecological security pattern for central China’s coal-grain overlapping areas and proposes a differentiated protection-restoration strategy characterized by “zonal management， categorized measures， and hierarchical governance”. By integrating ecosystem service importance assessment with circuit theory， we established an ecological security pattern for central China’s coal-grain overlapping areas. This framework was synergized with the “Henan Provincial Territorial Spatial Planning （2021-2035）” and “Henan Four Waters Co-Governance Plan （2021-2035）” to delineate critical ecological protection-restoration zones and formulate targeted strategies. The findings reveal that： 1） The spatial distribution of ecosystem service importance in Henan’s coal-grain overlapping areas exhibits a pattern of “high values in the east-west direction and low values in the north-south direction”， with ecological “source-corridor-node” elements showing uneven agglomeration. 2） A integrated framework was identified， comprising four different zones （plain water conservation and soil erosion control zones， plain groundwater over-extraction protection zones， mountain-hill water conservation and soil erosion control zones， and mountain-hill groundwater over-extraction protection zones）， three categories （key restoration， general restoration， and ecological conservation） and three priority levels （critical， important， and general）. 3） Significant disparities exist in regional challenges and restoration areas across different zones. This study improved the traditional ecological restoration zoning models by proposing an integrated strategy that combines zonal management， categorized measures， and hierarchical governance for the coal and grain overlapped area. This study provides practical methods and recommendations for ecological protection and restoration projects of territorial space， and offers critical references for ecological conservation and sustainable development in central China’s coal-grain overlapping areasKeywords：restoration and protection of national land space;ecological security pattern;coal grain overlapping areas;differentiated repair strategy;circuit theory52|16|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To investigate the effects and underlying mechanisms of low-carbon city pilot programs on the agricultural green total factor productivity， a theoretical framework was developed based on urban-rural integration development theory. The impacts of low-carbon city pilot policies on agricultural green total factor productivity were analyzed from three perspectives： factor integration， industry integration and technology integration. An panel data from 41 cities in the Yangtze River Delta region， covering the period from 2001 to 2022， were utilized in this study. The multi-phase Difference-in-Differences model was utilized to empirically evaluate the effects of low-carbon city pilot policies on agricultural green total factor productivity. The findings indicate that： 1） Compared to non-pilot cities， the agricultural green total factor productivity of cities designated as low-carbon pilot areas displayed a notable increase of 6.6%. The above conclusion remains robust even after conducting a thorough robustness analysis. 2） In the context of mechanism analysis， the low-carbon city pilot projects primarily influence agricultural green total factor productivity through the integration of urban and rural elements， industrial convergence， and technological advancement. 3） Based on the heterogeneity analysis concerning geographical location and resource endowment in the Yangtze River Delta region， the impact of low-carbon city pilot programs on agricultural green total factor productivity is more pronounced in peripheral cities and non-resource-based cities. Therefore， it is essential to expedite the integration of urban and rural elements， advance pilot initiatives for low-carbon cities， and foster the green development of agriculture.Keywords：agricultural green total factor productivity;new quality productivity;integration of urban and rural areas;the Yangtze River Delta region40|18|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To explore the impact mechanisms of internal control and government regulation on the adoption of green production technologies by farmer cooperatives， this study empirically tests the impact of internal control and government regulation on the adoption of green production technologies by cooperatives based on 585 planting production and operation cooperatives in Henan and Shaanxi provinces. The results indicate that both internal control and government regulation significantly promote the adoption of green production technologies by cooperatives. From the perspective of internal control， the effects of input control varies on different green technologies adoption behaviors， while process control and outcome control have significant positive effects on the adoption of certain technologies. Among government regulations， incentive， constraint and service regulations display promotion effects on the adoption of some green production technologies by cooperatives， but service regulation significantly inhibits the adoption of integrated water and fertilizer management technology. The interaction between government regulation and internal control shows significant substitution effect in the adoption of integrated water and fertilizer technology. The promoting effects of internal control and government regulation on the adoption of green production technologies are stronger for cooperatives with higher levels of independent operation and less standardized democratic decision-making. Based on these findings， it is recommended to strengthen internal control mechanisms in cooperatives and improve the targeting and effectiveness of government regulations to fully harness the potential of farmers’ cooperatives in promoting green agricultural development.Keywords：internal control;green production technologies;fertilizer reduction technologies;farmer cooperatives;government regulation20|18|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To promote the construction of the diversified food import system in coordination with the Big Food Concept， based on the explanation of the impact and mechanism of practicing the Big Food Concept on food imports， the data on production， trade， transportation， and national risks of major agricultural product exporting countries in the world are used to select key indicators for risk and potential analysis based on various links of the international food supply chain. The results indicate that： In terms of supply security， there is still some room for expansion in the import sources of staple crops， while the expansion space for the import sources of feed grains is limited. At the same time， it is necessary to be vigilant about the impact of annual production fluctuations on the stable supply of the international market. In terms of procurement safety， national risks and changes in trade policies of major exporting countries are the key factors affecting grain trade， and the impact of import costs on feed grain imports is greater than that on rations. In terms of transportation safety， it is necessary to comprehensively consider the transportation distance and route， and appropriately disperse the transportation capacity and the risk of transportation interruption and congestion caused by excessive concentration of routes. To promote the synergy between the concept of Big Food Concept and grain imports， it is necessary to further optimize and expand the scale， structure， methods， and sources of traditional grain trade， and consider the appropriate import scale of different varieties in combination with domestic demand. At the same time， the adjustment of agricultural product trade structure should meet the domestic food consumption needs， and promote the diversified layout of agricultural product import sources in different trade varieties. It can be extended from direct trade to the global agricultural food industry chain supply chain， and continuously improves the ability to utilize international market resources.Keywords：Big Food Concept;food imports;coordination;risk analysis;optimization strategy60|24|1<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
Influence of digitization and green synergistic transformation on food security guarantee capability
Abstract：Based on the provincial panel data from 2013 to 2022， this study empirically tests the impact mechanism and dependency conditions of digitization and green synergistic transformation on food security capabilities by adopting the fixed effect model， mediating effect model， and threshold effect model. The results show that： 1） The digitization and green synergistic transformation can significantly enhance the food security guarantee capability. The improvement effects in various dimensions， from strong to weak， are innovation transformation ability， adaptation and adjustment ability， and risk resistance ability. 2） In the main grain-producing areas， the central and eastern regions， digitization and green synergistic transformation have a more significant effect on enhancing the food security guarantee capability. 3） The digitization and greening synergistic transformation can indirectly affect food security guarantee capability by accelerating land transfer， promoting non-agricultural employment， and developing agricultural productive services. 4） The new agricultural management subject is an important dependent condition for the digitization and green synergistic transformation to improve the food security guarantee capability. Developing a digitization and green synergistic mechanism， optimizing factor allocation， and cultivating new agricultural business entities are proposed in this study to improve the food security guarantee capability and build a solid foundation for food security.Keywords：digitization;green;synergistic transformation;food security guarantee capability54|28|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06- Abstract：To clarify the impact mechanism of digital rural construction on rural revitalization and its coordinated development， this study takes Zhejiang Province， a national digital rural pilot zone and a provincial-ministerial co-constructed rural revitalization demonstration province， as the research object. Based on the county-level panel data from 2001 to 2023， the OLS regression model， mediation effect model， threshold effect model and other methods are used to empirically test the impact of digital rural construction on rural revitalization and the coordinated development of the“Five revitalizations”， namely industrial， talent， cultural， ecological， organizational revitalization. The results show that： 1） Digital rural construction can significantly improve the rural revitalization level and the coordinated development level of the “Five revitalizations”. After a series of robustness and endogeneity analyses，these conclusions remain valid.2） Digital rural construction can promote rural revitalization and coordinated development of the “Five revitalizations” through transportation infrastructure upgrade and technological innovation.3） Digital rural construction can significantly promote rural revitalization and the coordinated development of the “Five revitalizations” in both the 26 mountainous and non-mountainous counties of Zhejiang Province， and this promoting effect is more fully exerted in the 26 mountainous counties. Digital rural construction can have a significant promoting effect on rural revitalization and the coordinated development of the “Five revitalizations” from 2001 to 2003， 2004 to 2016， and 2017 to 2023， and this promoting effect gradually strengthens over time. The results of the heterogeneity analysis based on different dimensions of rural revitalization show that digital rural construction has a significant positive impact on talent revitalization， cultural revitalization， and organizational revitalization， a significant negative impact on ecological revitalization， and a non-significant impact on industrial revitalization. 4） There is a non-linear characteristic of diminishing marginal effect in the impact of digital rural construction on rural revitalization. Therefore， it is imperative to persistently implement the digital rural development initiatives， promote digital transformation of rural transportation infrastructure， strengthen investment in technological innovation， develop differentiated digital rural enhancement paths tailored to local conditions， and advance comprehensive rural revitalization.Keywords：digital rural construction;rural revitalization;transportation infrastructure;technological innovation;counties in Zhejiang Province43|26|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To explore the impact of the interest linkage between enterprises and farmers on the leading behavioral performance of leading agricultural enterprises， based on the panel data of 962 provincial leading agricultural enterprises in Jiangxi Province from 2020 to 2022， the fixed effect and the mediating effect models are used to demonstrate the influence of the interest linkage between enterprises and farmers on the leading behavior performance of the enterprise and its mechanism of action. The results show that： 1） The interest linkage between enterprises and farmers can significantly improve the leading behavioral performance of leading agricultural enterprises， which is specifically manifested in increasing the number of farmers driven and promoting farmers' income growth. 2） The interest linkage between enterprises and farmers expands the number of farmers driven through intermediary organizations such as farmers' cooperatives and family farms and increases in farmers' income growth through means such as agricultural product quality certification and the demonstration and promotion of agricultural science and technology. 3） In the samples of processing-type leading enterprises and enterprises in less-developed regions， the interest linkage between enterprises and farmers has a significant positive effect on increasing farmers' income. However， there are undistinguishing differences in terms of the number of farmers driven. Therefore， some suggestions are proposed as follows： Establish a stable and close interest linkage mechanism and encourage leading enterprises to invest in poverty alleviation areas； strengthen the support for intermediary organizations and guide them to form long-term and stable cooperation with leading enterprises； explore characteristic agricultural resources and support enterprises and farmers to carry out agricultural product quality certification； encourage enterprises to conduct in-depth processing of agricultural products and build a complete industrial ecosystem； and promote the integration of industry， academia， research and application to enhance the level of the demonstration and promotion of agricultural science and technology.Keywords：interest linkage mechanisms;degree of interest linkage;agricultural leading enterprises;leading behavioral performance;number of farmers;farmers’ income47|18|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：This study aims to investigate the paradox whereby facility vegetable farmers do not expand production scale despite high economic returns and rising output efficiency. Drawing on a set of survey data from 1 147 farmers across eight cities in Hebei Province from June 2023 to January 2024， nonlinear effect models and quantile regressions were used for analysis. The results show that： 1） There is an inverted “U” shaped relationship between the operation scale of facility vegetables and the output efficiency， and the optimal scale interval corresponding to the efficiency peak is 1.09 to 1.23 hm². 2） As the scale expands， farmers’ dependence on external labor increases， and the proportion， cost， and structural changes of hired laborers are significant. The hired labor presents a dynamic transformation mechanism in which “high-efficiency labor” and “low-efficiency labor” coexist， which is an important reason for the inverted “U” shaped relationship between the scale-efficiency of facility vegetables. 3） Brick-wall greenhouses and autumn-winter crops show early efficiency improvements， but the marginal cost rapidly increases when it exceeds thresholds. Plastic greenhouses and summer crops， due to environmental and output instability， are difficult to achieve sustained efficiency gains through scale expansion. Hired labor allocation efficiency is the core constraint on scale expansion， reflecting a trade-off between specialization benefits and rising management costs. Efficiency differences across facility types and crop seasons underline the importance to optimize labor use and improve management in facility agriculture.Keywords：facility vegetables;agricultural laborers;scale of operation;production efficiency21|19|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To clarify the relationship between agricultural insurance and agricultural green and low-carbon development， using the panel data of 30 provinces in China from 2005 to 2022， based on the policy perspective of full-cost insurance and income insurance pilot， this study empirically tests the influence effect and mechanism of pilot development on agricultural net carbon sink， and focuses on the synergy between green agricultural technology adoption， large-scale operation and planting structure adjustment. The results show that： 1） The implementation of the pilot policies of full cost insurance and income insurance has significantly improved the net carbon sink of agriculture； 2） The pilot policy mainly promotes agricultural carbon reduction and foreign exchange increase through large-scale operation， planting structure adjustment and green agricultural technology adoption， and there is synergy between green agricultural technology adoption and large-scale operation and planting structure adjustment to jointly promote carbon reduction and foreign exchange increase in the pilot areas； 3） The pilot policy has a significant impact on agricultural carbon emissions and carbon absorption， and has different impacts on carbon emissions from different carbon sources； 4） The pilot policy has a significant impact on the agricultural net carbon sink in the middle and high natural risk areas， but has no significant impact on the agricultural net carbon sink in the low natural risk areas. Based on the research results， this study puts forward some policy suggestions as follows： Speeding up the promotion of full-cost insurance and income insurance and improving farmers' willingness to participate in insurance； Optimize the premium subsidy mechanism and give play to the demonstration role of large-scale business entities and specialized growers； Improving green technology extension services and enhancing farmers' technology application ability； Building a differentiated support system for risk areas and strengthening subsidies and infrastructure investment in high natural risk areas.Keywords：full-cost insurance and revenue insurance;agricultural emission reduction and carbon sequestration;synergistic effects;progressive DID28|17|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：To explore the impacts of agricultural socialized services on the resilience of agricultural economy and its mechanism， this study is based on the panel data of 29 provincial-level regions in China from 2010 to 2023 （the data do not include those of Shanghai， Xizang， Hong Kong， Macao and Taiwan regions， the same below）. Entropy method is employed to determine the level of agricultural economic resilience across regions. Furthermore， the econometric models including a panel fixed-effects model， a mediation effect model， and a panel threshold regression model are construct to analyze the mechanisms through which agricultural socialized services influence agricultural economic resilience. The results indicate that the agricultural socialized services significantly enhance the resilience of the agricultural economy. These findings remain robust after conducting a series of endogeneity and robustness checks. The mechanism analysis reveals that agricultural socialized services contribute to economic resilience by increasing farmers' income and improving agricultural labor productivity. Heterogeneity analysis shows that the positive impact of agricultural socialized services is more pronounced in eastern China and non-major grain-producing regions. The threshold regression results suggest a nonlinear relationship between the influence of agricultural socialized services and the scale of agricultural operations， with greater effectiveness observed in areas characterized by smaller-scale farming. Based on these findings， policy recommendations including actively optimizing the institutional framework for agricultural socialized services， formulating region-specific development strategies， promoting high-quality service provision are put forward to ultimately enhance the resilience of the agricultural economy.Keywords：agricultural socialized services;agricultural economic resilience;farmers' income;agricultural labor productivity;scale of agricultural operation36|31|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
- Abstract：By using the CFPS database 2010-2022， the impact of rural left-behind experiences on workers’employment situations and career path choices is explored. The study finds that： 1） Left-behind experiences negatively affect workers’ employment situations and further influence their future career choices. 2） The longer rural children are left behind， the worse their employment situation will be in adulthood. 3） The older rural children are when they were left behind for the first time， the less optimistic their adult employment prospects will be. 4） Education level can partially offset the negative impact of left-behind experiences. 5） The implementation of national policies on child development has a positive improving effect on the employment quality of workers who have left-behind experiences.Keywords：left-behind experience;employment;employment quality;career choice241|50|0<HTML><L-PDF> <WORD> <Meta-XML>Updated：2026-05-06
