Issue Ebookcover storyIssue Cover
List of IssuesDocument Types
- Abstract：Determination of pleiotropism with QTL linkage benefits multiple traits improvement in plant breeding.A prototype of pleiotropic QTL detection for multiple-line crossed population was built basing on regression analysis, approximate likelihood ratio test, permutation test and parametric bootstrap.And the prototype is also suitable to biparental population.The prototype was used to map and determinate pleiotropic QTL with linked QTL for multiple traits of maize stalk in a RIL population constructed from inbred line B73 and Ce03005.All genotypes were evaluated at two locations in two years totally four macro environments for phenotypic traits.The results are shown as follows:There is one pleiotropic QTL on chromosome 5 controlling traits of moment of maximum bending force (MF) and internode diameter (d);QTLs affecting neutral detergent fiber of unit volume (NDF) and internode water content (Wwc) at bin6.01 and QTLs affecting Internode dry weight (WD) and MF at bin8.03 are also pleiotropic QTLs;However, on chromosome 5, QTLs controlling traits of d and NDF, and QTLs for traits of MF and NDF are linked QTLs but not pleiotropic QTLs.The QTLs determination is valuable to maize breeding and the analysis prototype is useful to other crop species.Keywords：maize;stalk;linked QTL;multiple traits;pleiotropic QTL3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Taking soybean cultivar Jinda78 and its mutation progeny M5 irradiated by 60Co γ as materials, coefficient of variation was utilized for genetic analysis of quality and 11 agronomic traits in mutation progeny.Grey relational analysis was conducted for correlation between quality and 11 agronomic traits in mutation progeny.SDS-PAGE was used for analysis of relative contents of β-conglycinin soy protein (7S), glycinin soy protein (11S), and their subunits.Furthermore, the correlation between protein and oil contents and the relative contents in mutation progeny were researched.The results showed that:Among the traits, oil and protein displayed the lowest coefficients of variation;Mainstem thickness, plant height, 100-seed weight and mainstem node number showed lower variation degree;Yield per plant, mainstem node number, 100-seed weight and mainstem thickness had higher relational degree with the contents of both protein and oil;The relative content of 7S showed significant and strong negative correlation with the relative content of 11S.All relative contents of the subunits of 7S and 11S showed significant and strong positive correlation with their relative contents;The relative content of 7S had positive correlation with both protein and oil contents.The relative content of 11S had positive correlation with protein content, and negative correlation with oil content.In conclusion, line 14 and 16 of Jinda78 M5 had lower relative contents of the subunits of α' and α.Therefore, these two lines can be used in special processing and plant breeding for cultivars without β-conglycinin soy protein.Keywords：soybean;60;Co-γ irradiation;quality;agronomic traits;correlation degree2|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The aim of this study was to investigate the fruit factors (such as the difference of varieties, fruit size, maturing status, et al) on CO2 de-astringency effect.The difference between 25 and 30℃ on CO2 de-astringency was firstly investigated, and no significant difference between 25 and 30℃ was found.Fruits of 60 astringent persimmon resources were then treated with 90% CO2 in 25℃, and de-astringency status, storage tolerance, morphological characters and contents of active ingredients were investigated.The results showed that the 60 persimmon resources were divided into 4 groups according to de-astringency status, and the group which was easiest to be de-astringent contain 30 resources, which could be de-astringent 24 h after the CO2 treatment.The resources were divided into 5 groups according to the storage tolerance.The results of correlation analysis suggested that fruit size andits maturing status were positively correlated to de-astringent status, whereas they were negatively correlated to storage tolerance.In addition, the content of soluble solid was negatively correlated to de-astringent status, and the content of total phenols, flavones and tannin were not significantly correlated to de-astringent status.In this study, 6 resources with good de-astringent status and storage tolerance were selected, and 11 resources with good de-astringent status and big fruit sizewere selected, and 8 resources with good storage tolerance and big fruit size were selected.It is also found that when the fruit size was bigger and the maturing status was higher and the content of soluble solid was fewer, the fruit is easier to be de-astringent.However, when the fruit size is bigger, the storage tolerance was lower.Thus, a tradeoff should be made among de-astringent status, storage tolerance and fruit size.Keywords：fruits of astringent persimmon;de-astringency status;storage tolerance;morphological characters;contents of active ingredients;varieties selection0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To establish "Qianbai" series cabbage regeneration-transforming technology system of cold resistance gene CBF3 and COR15A, "Qianbai" series 3 inbred lines of Chinese cabbage were used as the research object.Using the cotyledons of petiole, half leaf, hypocotyl of 4 d age seedlings of Chinese cabbage as explants, and the cotyledon petiole of Zui4 as the receptor of genetic transformation, we transferred the expression vector with bivalent cold resistance gene CBF3 and COR15A into Chinese cabbage by agrobacterium-mediated method.Factors affecting explants regeneration and genetic transformation of Chinese cabbage were also studied.By antibiotic selection, we obtained 6 transgenic plants by PCR from 36 T0 transgenic plants.Preliminary identification of cold resistance showed that the cold resistance of transgenic cabbage plants is higher than that of control plants.0|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Four nitrogen-fixing Paenibacillus strains 1-18, 1-33, 1-43 and 1-49 are used to inoculate wheat, and the wheat yields are analyzed to determine the effect of these strains on wheat yields.The results showed that wheat yields were increased by 26.9%, 9.7%, 5.1% and 18.7%, respectively.Furthermore, the nitrogenase activities and IAA amounts produced by these 4 strains were analyzed.Of these 4 strains, strain 1-18 exhibited the highest nitrogenase activity (1 043.6 nmol/(mg·h)) and the activity (969.5 nmol/(mg·h)) of strain 1-49 is higher than the rest 2 strains 1-33 ((565.9±3.0) nmol/(mg·h)) and 1-43 ((904.5±4.3) nmol/(mg·h)).However, the IAA amounts produced by strains 1-18 ((24.95±1.2) μg/mL) and 1-49 ((26.34±4.2) μg/mL) are lower than those produced by strains 1-33 ((27.73±1.8) μg/mL) and 1-43 ((49.91±3.6) μg/mL).Our study provided fundamental information for strain improvement of nitrogen-fixing strain for microbial fertilizer.3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To discover the pathogens causing alfalfa root rot in Hebei Province, China, and to provide references for prevention and control of the disease, isolation, identification and indoor pathogenicity test of pathogens from the samples of alfalfa root rot collected in Hebei Province were conducted, and toxicity determination of fungicides for controlling the disease was investigated.A total of 71 pathogenic strains were isolated from the diseased tissues and were identified as Fusarium strains by using the morphological identification method and molecular biological identification method with the ribosomal internal transcribed spacers (ITS4 and ITS5) and extension factors (EF-1H and EF-2T) as the primers for PCR amplification, respectively.The identification results obtained by using the morphological identification method and the molecular biological identification method were consistent.The results showed that the 71 strains of Fusarium included 55 strains of F.equiseti, seven strains of F.oxysporum, six strains of F.proliferatum, two strains of F.incarnatum and one strain of F.solani.The pathogenic determination results demonstrated that strain D19-2 of F.oxysporum, strain S45 of F.solani and strain Q1 of F.proliferatum had the strongest pathogenicity among all of 71 strains. The sensitivity of the three strongest pathogenic strains (including D19-2, S45 and Q1) to 40% myclobutanil wettable powder (WP), 50% carbendazim WP, 99% hymexazol WP, 40% dimetachlone WP, cyprodynil·fludioxonil (37% cyprodynil and 25% fludioxonil) water dispersible granule (WDG), 50% thiram WP, 80% mancozeb WP, 70% thiophanate-methyl WP and 10% difenoconazole WDG, was determined using the poison medium method.The results indicated that 40% myclobutanil WP, 50% carbendazim WP, cyprodynil·fludioxonil WDG, 50% thiram WP, 70% thiophanate-methyl WP and 10% difenoconazole WDG had better inhibitory effects on mycelia growth of the three strains than the other three types of fungicides.Keywords：alfalfa root rot;Fusarium;pathogen identification;fungicide;toxicity determination2|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：An investigation was conducted with a super heavy oil sample as sole carbon source to select highly efficient hydrocarbon-degrading strains among isolates from oil production water and analyze their degradation properties for oil four components and total hydrocarbon components.The results showed that:Strain H11 and W14, which belong to Pseudomonas aeruginosa based on 16S rRNA gene sequence analysis, could emulsify super heavy oil thoroughly;the relative contents of aliphatic hydrocarbons were reduced by 20% after incubation with these two strains;furthermore, the two strains could degrade long chain hydrocarbons with C26 and C28 as the longest ones, respectively.These data suggested that strain H11 and W14 could be reliable resources to emulsify and degrading heavy oil and hydrocarbons in oil- and farm chemical-contaminated environments.Keywords：hydrocarbon-degrading strain;Pseudomonas aeruginosa;oil- and farm chemical-contaminated environments;bioremediation2|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To investigate the effects and molecular mechanisms of HH signalingpathway in the adipogenic differentiation of adipose-derived mesenchymal stemcells (ADSCs), porcine ADSCs was established and the HH signaling pathway was activated with continuous treatment by purmorphamine.The adipogenic differentiation of ADSCs was analyzed by oil red O staining.The expression of Gli1 and adipogenic transcription factors was checked by Real-timePCR and Western blot.The results showed that:HH signaling pathway was activated bycontinuous treatment with 5 μ mol/mLpurmorphamine (PM);The expression level of Gli1 mRNA and protein wasincreased;The number of adipocytes and the content of triglyceride were decreased during adipogenic induction differentiation of porcine ADSCs;In addition, CCAAT/enhancer-binding protein-α (C/EBP α) and peroxisome proliferator-activated receptor-γ (PPAR γ), the adipogenic transcription factors, were repressed by PM.The result indicated that the activation of HH signaling pathway inhibits the adipogenic differentiation of porcine ADSCs, and its inhibition may be due to reducing the expression of C/EBP α and PPAR γ.Keywords：pig;HH signaling pathway;purmorphamine;ADSCs;adipogenic differentiation2|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To investigate the effects of colistin sulfate on cecal microflora and immune organs of TLR2, TLR4mRNA relative expression and the content of peripheral blood cell factor in Rex Rabbit.Thirty rex rabbits of 35 days old were randomly divided into two groups.Group Ⅰ (Control group) was fed the basic diet.Group Ⅱ was fed with the basic diet containing 20 mg/kg colistin sulfate.PCR-DGGE and Real-Time PCR were applied to analyze the differences of cecum microflora, contents of cytokines in peripheral blood were detected by ELISA and real-time PCR was used to detect the expression of TLR2 and TLR4 mRNA relative expression.The diversity of cecal microflora in group Ⅱ was similar to group Ⅰ (P > 0.05).The numbers of Clostridium cluster XIVa, Enterococcus spp., and Streptococcus spp.in the caecum of group Ⅱ were superior to those of group Ⅰ (P < 0.05), and Clostridium cluster Ⅳ was significantly lower than that in group Ⅰ (P < 0.05).The addition of colistin sulfate increased the relative expression of TLR2/4 mRNA in appendix and TLR4 in ileum, but only the TLR4 of the caecum was significantly different (P < 0.05).Furthermore, Spleen index and relative expression of TLR2/4 mRNA in group Ⅱ were higher than group Ⅰ, while a decreasing trend was observed in liver index and relative expression of TLR4 mRNA.However, there was no significantly difference (P>0.05).Liver TLR2 was increased significantly (P < 0.05).In Addition, the concentration of IL-4 in peripheral blood was higher than that in group Ⅰ (P < 0.05).The basic diet with 20 mg/kg colistin sulfate could promote the development of the spleen and improve the body immunity in Rex Rabbit.Keywords：rex rabbit;colistin sulfate;organ index;cecal microflora;TLR;peripheral blood cell factor2|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The color of the images for the same leaf look inconsistent when they are taken under various illuminations or weather conditions.Ten typical color constancy algorithms are used to process grape leaf images and their effects on color restoration were assessed in both color difference Δ Ea*b* and the angle error of the estimation source methods.The results showed that:Both methods worked to some extent, which 2nd-order Grey-Edge and Edge-based Gamut Mapping achieved the best results regardless of various illuminations, weather conditions or grape cultivars;And, from the visual aspect, the leaf color of images processed by both algorithms was closest to the reference image.The data indicate that:The 2 color consistency algorithms are of great help to solve the problems of color variety in the images incurred by different imaging conditions and to realize the color recovery of grape leaf images.Keywords：grape;leaf color;image processing;color consistency algorithm;consistency recovery2|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To decrease the electricity power used in rut root-cutting, 16 blades of four different types of edge curves (logarithmic spiral, Archimedes spiral, exponential sine curve and eccentric circular arc line) were designed according to the requirement of grassland root-cutting and the change regularity of the sliding cutting angle.Through soil-bin experiment, the pull force and rotation torque of the designed blades were measured, power consumptions were calculated.The relationship between and parameters of the four types of the edge curves was simulated, and the best combination of the parameters of each edge curve was obtained for the smallest power consumption.By comparing the power consumption of different edge curve under the best combination of all parameters, the most suitable edge curve was identified.The result of the experiment optimization indicated that the lowest power consumption reached 3 345 kJ/m3 when the edge curve was exponential sine curve which its beginning static sliding cut angle was 40° and the proportional coefficient between the increment of the static sliding cutting angle and the polar angle was -0.1.Keywords：root-cutting;edge curve of blade;soil-bin experiment;specific work3|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Winter wheat cultivar Shijiazhuang 8 was taken as research material, the growth and development stage, yield and yield components, biomass, soil water content, soil nitrate content and nitrogen uptake amount of CERES-Wheat model under different irrigation conditions in North China Plain were analyzed integratively.The results showed that the simulation effects of growth and development stage, yield and biomass were very good under water saving and rainfed conditions.However, it was only good in the simulation of growth and development stage, but not other biological characteristics under the excessive irrigation treatment, and the model overestimated the contribution of irrigation amount on yield.In conclusion, the CERES-Wheat model can simulate the dynamic change of soil water under different irrigation conditions, but its stability needs to be improved.Furthermore, the performance of the CERES-Wheat model was good in the simulation of soil nitrate amount in 2 m depth soil profile, but not in plant nitrogen uptake amount.Therefore, further modification is needed to improve the systemic precision of the model.Keywords：CERES-wheat model;irrigation level;yield and yield components;biomass;soil water;plant nitrogen uptake amount;soil nitrate amount;simulation effect0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Using the data from China Agricultural Statistics and China Agriculture Yearbook, the potential of national and regional crop straw resource utilization in 2012 is estimated in this study.The differences of the crop straw utilization potential among regions based on the density of the crop straw are analyzed.Market development potential of crop straw resource utilization is also discussed.The results show that the total amount of crop straw resources in China on 2012 is 8.486×108 t, of which 6.406×108 t crop straw resources can be collected and utilized.Except traditional consuming, such as returning to the farm land, burning as family fuel and feeding animals, 1.515×108 t crop straw resources can be turned into biomass energy.The differences in crop straw resource utilization are existed among different region in China.The middle-lower reaches of Yangtze River, northeast China, and northern China, have larger crop straw resource potential that can be used as biomass energy, which are 4.451×107, 3.626×107 and 3.511×107 t respectively.While the potential in southern China, Loess Plateau and Qinghai-Tibet region is lower.The potential resource density in the middle-lower reaches of the Yangtze River, northern China and northeast China is relatively concentrated.Therefore, it is suitable to build large-scale energy companies, such as the direct combustion, crop straw gasification, in these regions.Strategies on enhancing local crop straw collection and storage system to form a relatively mature market on crop straw resource utilization and continuously improving the level of crop straw resource utilization are suggested at the end of this paper.Keywords：crop straw resource utilization;resource density;potential estimation;market evaluation0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on the data of villages and towns in Yuzhong County in 2008 and 2012, this study analyzed the level and spatial characteristics of comprehensive development of villages and towns in Yuzhong County in 2008 and 2012, assessed their degree of coordinated development and discussed their directions and strategies by the methods of the spatial analysis and mathematical statistics.The main conclusions were as follows:1) The spatial characteristics of the comprehensive development and coordination development of villages and towns in Yuzhong County were "west area>east area", "middle area>south and north area" and south area was slightly higher than that in north area.Moreover, the development level of villages and towns decreased gradually from the Central Mountainous Plateau Valley Area to the Southern Alpine Mountainous Area and the Northern Loess Arid Mountainous Area;2) On the whole, the development of villages and towns in Yuzhong County in 2008 was at medium level.Compared with 2008, it was increased on 2012 with more villages and towns at high level of development and fewer villages and towns at low level of development.3) In general, the coordination degree of development of villages and towns in Yuzhong County was low, one third of the villages and towns were in the state of non-coordination.However, the coordination degree was improved from 2008 to 2012;4) Researches on the actual development situations and conditions of villages and towns in Yuzhong County, provided scientific basis for rural construction and sustainable development of Yuzhong County and the development direction and measures were put forwarded.Keywords：villages and towns;comprehensive development evaluation;regional coordination development;Yuzhong County2|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The purpose of this paper is to provide theory basis and method for reference to construct the evaluation index for comprehensive land resources dominance of levied land scientifically, which might provide practical reference for the government or the development zone to formulate planning of schedule and scale of land supply.The method consisted in this paper by means of GIS (Geographical Information System) with integrated application of literature research, field investigation and empirical studies.The paper has constructed the evaluation index system for comprehensive land resources dominance of the levied land with 4 evaluating target and 15 indicators, corrected the index weight with dynamic weight which was set up according to the different land use type, then calculated the comprehensive land resources dominance with the weighted average mode.The results of this study showed that the largest area of land supply was land for industrial development, and the supply of land was first increased and then decreased;Secondly for the urban construction land, the supply of land increased year by year;At least for public services, reduced year by year.Keywords：land resources;dominance evaluation;land supply;schedule and scale;industrial development zone in Liangjiang New Area6|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Quantitative analysis was adopted to understand a rural settlements spatial distribution in Longnan County of Gannan hilly area and provide the effective reference in Longnan County residential renovation and village planning.The rural residents in Longnan County in 2013 and DEM vector data were taken as study object.GIS spatial method and Voronoi diagram Cv value analysis method combined with kernel density function were used to explore the Longnan County residential spatial distribution characteristics and influencing factors.The results showed that:The overall Cv value reached 147.81% in Longnan County;In addition to Anjishan reclamation farm, the remaining 14 townships of the Cv value is greater than 64%, The spatial distribution of residential space is obvious, showing a large gathering and small dispersion characteristics;Kernel density analysis found that residents of Longnan County spatial layout showing "north intensive south sparse" pattern, the average residential density is 1.66/km2;Residents of Longnan County spatial layout are relatively strong Influenced by elevation, slope direction, slope, road and river water, Showing obvious terrain and traffic direction.Keywords：rural settlement;Voronoi diagram;kernel density estimation;spatial distribution3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on monthly data obtained from January 2001 to December 2013 monthly data, the impact of international crude oil and corn-based fuel ethanol prices volatilities on the China's corn prices were analyzed by employing the VEC model and OLS model.The empirical results showed that there was significant integration relationship between international energy market and China's corn market.When International crude oil prices rose by 1%, domestic corn prices increased by 0.4332%.However, the impact of corn-based fuel ethanol prices volatilities on China's corn prices was insignificant.Meanwhile, the results from sensitivity test also proved the robustness of the VEC and OLS regression results.Keywords：energy prices;corn prices;integration relationship;sensitivity test0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Using the multinomial Logistic model, rural environmental satisfaction and its influence factors in the northeast cold region based on 1 545 effective questionnaire from Heilongjiang, Jilin, Liaoning and eastern Inner Mongolia region were analyzed.The results showed that:Peasants in Heilongjiang Province gave out the highest environment satisfaction evaluation value while there was large satisfaction evaluation differentiation among the other three provinces;Environment satisfaction evaluation value was the lowest and the differentiation is the highest in the mining area while the value is higher in forest and grass area;People who are uneducated or have a college degree or above, teenagers younger than 20 years old and the elderly over 60 displayed higher satisfaction to the environment.The Logistic analysis uncovered that the drinking water quality and atmospheric quality played an extremely significant role to the rural environmental satisfaction.The soil pollution degree, surface water quality, environmental law cognition, green infrastructure and government investment are important factors, and 7 other factors, sewage discharge way, domestic waste treatment, human and animal manure treatment etc., also had significant effect on rural environment satisfaction.Keywords：rural environment satisfaction;influence factors;Logistic analysis;severely cold region of Northeast China0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
