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- Abstract：A primary cortical neuron was separated and cultured from a new rat to provide a basal material for detailed study of pathological changes in the central nervous system.We obtained the cerebral cortex of the new rat by aseptic operation.Trypsinization and 5-fluorodeoxyuridine was used for harvesting and processing of the neurons.Neurons were identified by Toluldine blue dyeing and symbolic enzyme NSE.The cultured nerve cell developed satisfactorily with a purity >90%,hence,it can be used for the next research as a model cell.0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The omp-1 DNA is known to be a strong immunogen and codes the 40 ku major outer-membrane protein(MOMP),an immunodominant antigen of the Chlamydiaceae surface membrane.A reliable and efficient molecular vaccine against swine Chlamydophila abortus Cp.abortus can be developed if a new approach for increasing immune response can be found.In the current study,the omp-1 of CP12 strain of Cp.abortus was amplified by PCR and cloned into pcDNA3.1( )to construct recombinant eukaryotic expression vector.BALB/c mice were randomly divided into five groups and inoculated twice weekly with different combinations such as DNA/r-MOMP,r-MOMP/DNA,DNA-r-MOMP,DAN/DNA and r-MOMP/r-MOMP.Two weeks post inoculation,the antibodies against MOMP were assessed by ELISA,and proliferation of T lymphocytes was monitored by Lymphocyte Transformation Test.Results showed that significantly higher immunoreactions could be induced in mice inoculated simultaneously with DNA and r-MOMP.Mice group primed with DNA and then boosted with r-MOMP exhibited higher level antibody and lymphocytes stimulation index as than that inoculated with r-MOMP,then with DNA.Reliable and efficient immunoreactions cannot be achieved either by inoculation with naked DNA or with recombinant MOMP.Nevertheless,a better immune response can be obtained if mice are administered with both DNA and its corresponding recombinant protein simultaneously.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：This study was designed to assess the effect and mechanism of action of selenium compounds and vincristine(VCR) on mice bearing Ehrlich ascites carcinoma(EAC),a tumor-bearing mice,and compare the toxicity of a single drug with combination of two drugs.An EAC mouse model was established by injecting tumor cells intraperitoneally and drugs were given to mice on the 7th day after injection.The survival time of Ehrlich ascites tumor-bearing mice was recorded.The protective effect of selenium compounds on mice carrying tumor was evaluated by antioxidant indices(SOD,GSH,MDA) and their effects on tumor cells apoptosis evaluated by flow cytometry and DNA ladder.Selenium compounds,especially methylselenocysteine(MSeC) combined with VCR significantly(P<0.01) prolonged the life span of tumor-bearing mice,improved the antioxidant level in vivo,and simultaneously promoted the apoptosis of tumor cells.In general,selenium compounds combined with VCR played a powerful role in protection of tumor-bearing mice.This mainly depended on the anti-oxidant function of selenium but the anticancer activity of VCR was also enhanced in combination with selenium,by inducing apoptosis.1|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Crop water requirement is an important parameter in irrigation scheduling.And the crop coefficient is a key to estimate crop water requirement by multiplying with the reference crop evapotranspiration.Crop coefficient was always be approximated from crop developing days.The method must have some defections because crop coefficient is a parameter related to crop growth status,climate condition and surface albedo.Since the crop growth status and some other factors relating to crop coefficient are spatial heterogeneous and remote sensing has advantage in obtaining the distributing parameters for vegetation and climate factor.In this paper,based on the Penman-Monteith equation,the reference crop evapotranspiration and potential evapotranspiration for cotton under different growth status was estimated with measured meteorological data,the crop coefficient for cotton was retrieved from a Landsat ETM image and the sensitivity of crop coefficient to the influence factors were analysed.The results showed that: the crop coefficient retrieved from ETM image was greater than the ones suggested by FAO and crop coefficient was influenced and decided by NDVI that represented crop growth status;while surface albedo that had a very larger variance for the sparse vegetation cover had scarcely any effect on crop coefficient and the climate factors had litter influence on crop coefficient too;with the vegetation cover fraction developing,the climate factor had much more positive effect on crop coefficient.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：For inspecting the quantity of nematodes in different trophic groups,especially that of those had not been listed in the quarantine organisms,the inspection on the nematodes imported together with corm flowers was carried out in this study with a new 7-day natural separation method created in our laboratory.The results shown that.1) The nematodes isolated from imported corms of lily,tulip and other 4 kinds of corm flowers from Holland and New Zealand were classified into 3 orders,10 families and 12 genera,among which un-phytoparasitic nematodes were classified into 1 order,3 families and 4 genera.Most of unlisted quarantine plant parasitic nematodes in the varieties of lily corms belong to the genus of Aphelenchoides(accounted for 77.1% of total isolated plant parasitic nematodes),Aphelenchus(16.4%) and Pratylenchus(2.9%).2) The amount of nematodes in Aphelenchoides genus found in lily corms from New Zealand(426 nema./10 corms)were 3.5 times as that from Holland(123 nema./10 corms).3) There were three imported varieties of lily corms carried Meloidogyne spp.,which are potential quarantine nematodes in China,but the quantity of those was small,between 2 to 7 individuals per 10 corms.The corms of the three varieties not only carried the potential quarantine nematodes but also took un-plant parasitic nematodes which belong to the genus of Panagrolaimus,Acribeloides, Cephalobus and Rhabditis.The amounts of these un-plant parasitic nematodes were between 50 to 300 nematodes per 10 corms.0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The effects of four types of liquid pesticides(imidacloprid,acetamiprid,imidacloprid DDVP and DDVP omethoate) on some protective enzymes and dissociative proline in weeping willow(Salix babylonica Linn) leaves through trunk injection were reported.The result showed that the activity of SOD experienced a raising-descending-raising process after a trunk injection.Among four pesticides,DDVP omethoate affected the activity of SOD greatly,the activity was increased 85.01% within one day,reduced 35.99% within six days and increased 32.00% within twenty days after a trunk injection.All of four pesticides caused the increase of PPO and PAL activity and the decrease of POD activity.Imidacloprid seriously affected PPO and POD activities,the activity of PPO was increased 21.87% within ten days after a trunk injection,and the activity of POD was reduced 24.78% within six days after a trunk injection.DDVP omethoate affected PAL activity greatly;the activity was increased 28.97% within six days after a trunk injection.The content of dissociative proline had also descended after treated by the four pesticides through trunk injection.DDVP omethoate affected the content of proline greatly;the content was increased 52.91% within three days after a trunk injection.The activity of SOD,POD,PPO,PAL and the content of dissociative proline had come back to the control level within thirty days after a trunk injection.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：One hundred and eighty 8-day-old avian broilers were used to investigate effects of isoflavone(SI),bentonite and glucomannan,on growth,immunity function and tibia.Feed intake and average daily weight gain were significantly decreased but the feed/gain ratio increased significantly(P<0.05) in broilers fed moldy corn diets than those fed less moldy corn diets.Both feed intake and average daily gain increased significantly while the feed/gain ratio was decreased substantially in broilers by adding 100 and 200 mg/kg SI.Also,serum IgA and IgG concentrations increased significantly(P<0.05).The average daily gain in broilers was significantly(P<0.05) increased by adding 0.05% bentonite or 0.05% glucomannan.However,calcium and phosphate levels in tibia were significantly decreased by adding 0.05% bentonite.In conclusion,soybean isoflavones more significantly increased growth of Avian broilers when added to a moldy corn diet than glucomannan or bentonite.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Peanut hill-drop planter mulching film,at present,in China adopts almost the same conventional operation,in which sowing is ahead of mulching film.The way of this working mode makes soil stirred and moisture transpired.So a rotary hole former for a small size peanut hill-drop planter with mulching film before sowing was studied in this work.The article analyzed the moving process of the mechanism and the expressions for width caved,distance of cave and depth caved were obtained.The main factors that influencing the working performance are crank radius,machine moving speed and crank rotate speed.The ratio of velocity is greater than 1 in natural condition.The computer aided analysis method was adopted to analyze the influences of structural and working parameters on working performance of the hole former.The optimized parameters are 6.5 of transmission ratio,0.045 m of crank radius,0.25 m/s of planter's moving velocity,under which the caved depth is about 4.5 cm,width about 3 cm and the distance of caves about 14.42 cm.The hole former can meet the requirements of peanut agricultural technology.The mechanism is simpler in structure and width caved is smaller compared with the common used hole former of duck mouth roll.4|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：A fractal pore network model for unsteady drying process of a natural porous body is developed based on the theories of fractal geometry,drainage and percolation through porous media and transport-process.Various phenomena,such as liquid-phase flow,vapor-phase diffusion,temperature gradient,as well as pore microstructure characteristic were considered into the mathematical model.To get a better accordance with a real pore network topological structure,a fractal pore network,instead of a regular pore network,was established.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The nitrite concentrations were measured,during the spontaneous fermentation and pure inoculation fermentation(with Lactobacillus pentosus and Leuconostoc mesenteroides as fermentation agent) of Chinese cabbage.It was found that the maximum nitrite concentration(nitrite peak) in the pure inoculation fermentation cabbage was two times lower than that in the spontaneous fermentation cabbage and the reason could be concluded from a serials of experiments as follows: enumeration of lactic acid bacteria,mesophilic aerobic bacteria and enterobateriaceae,changes of pH value and lactic acid concentration.Numbers of mesophilic aerobic bacteria(enterobateria and lactic acid bacteria not included) that could produce the nitrite peak in the pure inoculation fermentation cabbage was less than these of in the spontaneous fermentation cabbage.And the numbers of lactic acid bacteria and concentrations of lactic acid in the former was more than these in the latter.The decrease velocity of pH value of the former was lower than the latter.Hence,nitrite concentration in the pure inoculation fermentation cabbage was lower than that in the spontaneous fermentation cabbage.2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The tensor wavelet numerical method possesses the self-adaptability and higher precision.But it is suitable to the problems only restricted to partial differential equations defined in rectangle domain.A kind of new wavelet numerical method for solving 2D partial differential equations in polygon domain is proposed.Based on the combination wavelet precise integration method with the fictious domain method.In this method,the compact support property improves the calculation efficiency of the fictious domain method,which is helpful to solving the dynamic model in engineering such as the rill erosion model.0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to reduce the long time spent for scanning the database by using Apriori algorithm,which may descend the mining accuracy,the research on the sample operation and precision control with the help of frequent item-set,especially,the frequent 1-item-set is presented in this paper.The HAC algorithm based on sampling was designed.The results in theory and capability experiment indicated that HAC algorithm could decrease the scanning times by at least once,promote the efficiency of mining and improve the computation precision.1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The elastic impact procedure of a beam under lateral impact of ball was simulated by using numerical method in which the contact deformation was considered.From calculation results,the effects of rigidity of ball and beam,velocities and mass ratios on the impact load were analyzed.The impact load results show that there is reasonable ascending part in the numerical results when the contact deformation is considered.The smaller the elastic modulus of the ball,the smaller the peak value of impact load and the longer the contact time.When mass ratio is constant,the peak value of impact load is proportional to the velocity.The smaller the velocity of the ball,the longer the ascending and descending part of one contact and the shorter the interval between neighboring contacts and neighboring descending and ascending parts.When the velocity is constant,the bigger the mass ratio,the smaller the peak value of impact load and the longer the duration of no contact of moving ball,the shorter the ascending and descending part.2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Based on the field land use survey data in country residential areas of a small town,Cixi,by using the general evaluation method,the conditions of intensive land use in country residential areas of small towns were evaluated.The results showed that the proportion of general intensive land use was the largest in Sanjiangkou village,secondly was in shape of disintensive land use,and the intensive land use level of reserved buildings were better than that of removed.It can be concluded that the intensive land use level of reserved buildings had been improved.On the other hand,it showed that there was a large intensive land use potential in country residential areas of small towns in China.Therefore,it is very important and necessary to pay more attention to the quality and improve the general production capability of agriculture in the future new village construction.0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
