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- Abstract：To understand the role of myo-inositol oxygenase gene in plant under water stress using RT-PCR,the cDNA of open read fragment of putative myo-inositol oxygenase gene(MIOX) in rice was successfully amplified to 927 bp in length and encoded 308 amino acids,named OsMIOX.Sequence analysis indicated that OsMIOX shared high homogeneity with the Arabidopsis thaliana MIOX gene.The cloned cDNA of OsMIOX gene was introduced into pCAMBIA-1301 vector,giving a plant expression vector containing OsMIOX gene linked with 35s promoter.Results of real-time PCR analysis showed that the OsMIOX gene was upregulated by PEG stress in upland rice cultivar IRAT109,and the expression level was higher in upland rice(IRAT109,Haogelao) than in lowland rice(Yuefu,Nipponbare) under PEG stress.This showed that the molecular mechanism under water stress was different between lowland rice and upland rice,perhaps due to differences in drought stress.The possible role of OsMIOX gene under drought stress was discussed.3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To explore the character and mechanism of Pinus massoniana bark extract(PMBE) inhibiting the growth of human colorectal carcinoma LoVo cells in vitro.MTT,microscopy,gel electrophoresis and flow cytometry were used to identify the characters of PMBE inhibiting LoVo cell growth and inducing apoptosis.Immunohistochemistry analysis and RT-PCR were used to explore the related molecular mechanism.PMBE inhibits the growth of LoVo cells in a time-and dose-dependent manner.PMBE treatment resulted in cell cycle arrest at G1/S phase,Sub-G1 curve,genomic DNA ladder pattern,and morphological changes,including nuclear condensation,boundary aggregation or split,and apoptotic body formation.In addition,PMBE up-regulates the expression of p53 and p21 and down-regulates the expression of Bcl-2.PMBE inhibits the growth of LoVo cells in vitro via double mechanisms of cell cycle arrest through up-regulation of p53 and p21 expression and apoptosis induction through down-regulation of Bcl-2 expression,which laid foundation for further research of the related signal transduction pathways.0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A novel pathogenesis-related class-10 protein(AmPR-10) from Astragalus mongholicus was purified and characterized.The purified protein appeared as a single band with a molecular mass of 17.2 ku on SDS-PAGE and was eluted from a Superdex-75 column with an apparent molecular weight of 32.6 ku.AmPR-10 was a glycoprotein with a neutral carbohydrate content of 13.7%.The carbohydrate was mainly composed of 73.0% arabinose and 15.0% glucose.N-terminal amino-acid sequence of AmPR-10 was significantly homology to pathogenesis-related proteins from the PR-10 family.The sequence had 80% identity with L1PR10.1C,a PR-10 protein from Lupinus luteus.AmPR-10had ribonuclease activity of 74.11 U/mg protein,as some other PR-10 proteins.0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Mopanshi was crossed as a female parent with Ze njimaru and Taishu,the Japanese nonastringent cultivar,and the resulting hybrids were studied.Only a few seeds(about 8%) were normal,but more than 90%,were abnormal.More than 80% of abnormal seeds were induced by protuberance around the hilum.Histological and morphological observations for improving germination rate and use of the germplasm resource,showed that: 1) Protuberance occurred at the aperture between two neighboring locules 40 d after pollination,frequently with black spot production.2)The protuberant endosperm cells were characterized by large degenerate nucleus and thin,usually collapsed cell wall,45 d after pollination.3) Comparatively, the protuberant seed grows faster,inside which,the endosperm for seed plumpness and an embryo that develops 5 d faster than normal seed exhibits vigorous differentiation and growth,132 d after pollination.Our primary conclusion is that the protuberance was produced in an aperture between two neighboring locules following pressure between fast growing seeds and limited space between locules.3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To overcome incompatibility caused by pre-fertilization barriers in lily breeding,we used two species and three hybrids of lily,to study use of cut-style pollination to overcome incompatibility associated with self-pollination and intraspecific hybridization.The cut-style pollination was achieved by applying 0.1?,0.1%NAA and a liquid medium of pollen culture to the stigma and observing the influence of style length on the production rate of ovules.About 30 ovules were obtained with cut-style pollination during self-pollination of 'Sorbonne',significantly more than CK.But,the production rate of ovules decreased from more than 30 to less than 10 in 6 intraspecific crosses which were compatible;some of those crosses formed no capsule even with the same method.Only 8 interspecific capsules were obtained with liquid medium of pollen culture,which were 6 more than CK,but no interspecific ovule was obtained.When longer pollen tube forming varieties were used as female parents in 4 intraspecific reciprocal hybridizations,the rate of production of ovules was higher.In 2 other intraspecific crosses with the same female parent,more ovules were obtained when shorter pollen tube forming varieties were used as male parents.The results demonstrated that application of cut-style pollination can overcome self-incompatibility of 'Sorbonne',but have no effects on intraspecific hybridization.When longer pollen tube forming varieties were used as female parents in intraspecific reciprocal hybridizations'Casa Blanca'and'Sorbonne'and 'Casa Blanca'and'Siberia',the efficiency of fertilization was not hampered.0|5|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To improve the water efficiency in turf management,we studied the effects of mowed heights(5,10,15 cm and no mowing) of turf grass on the harvested dry biomasses and the water consumptions with 3 grass species(Poa pratensis,Festuca arundinacea,Lolium perenne) using portable lysimeters under full water supply condition.The results indicated that water consumption increases with the grass height but decrease for the harvested dry biomasses though their reduction quantities were varied for different species.The average harvested dry biomass for grasses was 1 661 kg/hm2(under 5 cm mowed height),1 345 kg/hm2(under 10 cm mowed height) and 972 kg/hm2(under 15 cm mowed height) respectively.The reductions in water consumption for grasses mowed to 5 cm height are 36.78%(Poa pratensis),34.02%(Festuca arundinacea) and 33.54%(Lolium perenne) respectively and lower than those for the un-mowed grass.The results show that grass mowing can reduce water consumptions and increase harvested dry biomasses.2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The change in rice production system is becoming essential due to water constraints.The traditional flooded cultivation has shifted to upland cultivation.Up to now,the area of cultivation of upland rice is only 2% of total rice cultivation area.However,iron deficiency in upland rice production is widespread,which limits improvement of the yield and quality of upland rice.The reasons leading to the Fe deficiency in upland rice remain unclear.One hypothesis is that the shift from ammonium to nitrate nitrogen(N) in soil induces Fe deficiency.The aim of this study was to elucidate the effect of ammonium and nitrate supply on rice growth and Fe nutrition and to make some comparisons on response to nitrogen form between upland and lowland rice.The results showed that Fe application enhanced the growth and Fe uptake of both rice types.Compared with ammonium N,nitrate N caused Fe chlorosis plants under Fe deficiency.Ammonium N better improved plant height and shoot dry weight and Fe uptake than nitrate N.The leaf SPAD reading,Fe concentration in shoots and roots and uptake were significantly higher in rice treated with ammonium N,especially when Fe was not supplied.The translocation of Fe was different between two nitrogen forms,especially for lowland rice.Ammonium N application induced more Fe translocation to shoots of lowland rice.Nitrate N accelerated Fe deficiency in plants,including decreasing leaf SPAD reading and Fe concentration when Fe was not supplied.All these results indicated that ammonium N was a better N source for the growth and Fe nutrition of upland and lowland rice.The dominance of nitrate N in upland soils was one of the reasons for Fe deficiency in rice production.1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Taken the Nonghua 5 peanut as experimental material,the effects of low energy C ion implantation in four doses in M3 generation on drought resistance was experimentally studied.The main stem,the cotyledonary branch,the dehydration rate and other parameters were observed.The methods of direct evaluation,comprehensive indirect evaluation and the clustering analysis were used to evaluate the drought resistance of M3 generation.Results showed that the drought resistance was distinctly inflected by low energy C ion implantation.In the dose of 1015 cm-2 the drought resistance was obviously prior to the control and it was slightly prior to in the dose of 1012 cm-2.While in the dose of 1011 cm-2 and 1016 cm-2 the drought resistances were almost no change.It can be also indicated from the results that the low ion implantation was an effective method to improve the seeds.2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A drying experimental study of potato slices,as an example of a real porous body,was conducted and its numerical simulation was carried out under the same environmental condition.The drying dynamic character of potato slices was obtained by the simulations of both a fractal pore network model and a regular one.The experimental and simulation results indicate that the drying kinetics, as well as the distributions of moisture and temperature inside the porous body,from the fractal pore network model are more consistent with that obtained from the drying experiments than that from the regular one.The wet patch appeared during the simulation process and this similar contour was also observed on the potato slices in the experiments.The throat size distribution in pore network of the porous media affects the drying process dramatically during the simulations.The higher the uneven degree of the throat size distribution was,the longer the drying time.There was no certain correlation between the pore fractal dimension and the drying time.0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A new method for leaf area measurement based on the algorithm of image characteristic value was studied in this research.Leaf area was measured based on the distribution of the scanning picture pixels and the histogram of the picture gray value.RGB tricolor gray value was separated from the picture and blue was selected as image characteristic value.And then the leaf area was determined by measuring the proportion of every leaf pixel.When green shredding units,with known total area,was measured by this method,the relative error of the results between the calculated area and the actual one was less than 0.5%.The correlation coefficient of leaf area measured by the traditional copy method and the scanning method was extremely high(r=0.997 1).11|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To solve the problem of object extraction using methods of traditional threshold value segmentation,which exists in obstacle detection technology based on stereo vision of automatic drive or assistant-driving of intelligent agricultural machinery,a new arithmetic for obstacle detection based on the combination of region segmentation and characteristic matching on the scanning line is proposed in this paper.The farmland image is acquired,centered in the binocular vision system,segmentalized by analyzing the distribution of pixel on the scanning line and extracted the object region after arrangement.Then,after a fast stereo characteristic match on object region aiming at obtain its dimensional message,it is able to detect the obstacle.An experiment of detecting the image of 800 frames(400 pairs) had been done and the experimental result indicated that the average time of processing each pair was not beyond 100 ms and the accuracy of spotting the obstacle reached 95%.The result also showed that this arithmetic could get the location of the obstacle effectively and had a good performance in farmland obstacle detection.2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on GIS software development technique,a soil moisture content monitoring and forecasting system for Beijing area was designed.The system has many functions,such as soil moisture collection,soil moisture sites supervision, soil moisture content monitoring and forecasting,soil moisture content information output and so on.By using this system the soil moisture content in Beijing can be monitored and the distribution map and the isoline chart of soil moisture content can be plotted in time.At the same time,three forecasting models including a soil moisture increasing and decreasing model,a time series model and an artificial neural network model were used to predict soil moisture content.One hundred and twenty mobile soil moisture sites and thirty three immobile soil moisture sites have been set up and begin to work,which show that the system will decrease the investment,enhance forecasting veracity and provide reliable and scientific foundation against drought in Beijing.9|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The finite element method is used to simulate the instantaneous response of a high-rise building with a sudden invalidation of side columns of the ground floor.Conclusions can be drawn from the calculation results that the instantaneous horizontal displacement of the structure fluctuates around the static result and converge to the static displacement because of the influence of damp.The biggest displacement of the instantaneous response is much bigger than that of the static one.The effect of initial static load on the instantaneous response makes the amplitude of vibration of horizontal and vertical displacement bigger than that without the effect.The effect of initial static load on the instantaneous response is related to the stiffness,mass and damp.The smaller the stiffness and the bigger the mass and the damp is,the smaller the effect of static load on the maximum displacement is.0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The anti-drift principle and drift-reduction rate of double-foil shield spraying technology was studied in this paper.In order to analyze the effect of nozzle setting position(horizontal distance,vertical distance) and spray angle,the effect on drift rate of nozzle type,nozzle size and air velocity on drift rate,the velocity field and droplet trajectory in and around double-foil shield were simulated under different conditions by using the CFD software.The simulated result showed that the anti-drift principles of the shield are: 1) high-speed downward airflow and low-speed horizontal airflow was formed under double oil shield(the maximal speed is 7.42 m/s);and 2) air turbulence intensity behind the shield was obviously weakened and a big area of negative horizontal and negative vertical velocity fields was formed.Moreover,the regression result suggested that there was a high correlation relation among nozzle setting position, spray angle and drift rate and the correlation numbers were 0.974 and 0.981 among air velocity,nozzle size,nozzle type and drift rate.In a word,double-foil shield spraying technology could effectively reduce drift rate and increase deposition rate.2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
