List of IssuesDocument Types

Volume28,Issue4,2023
Issue CoverIssue Contents
  • Effects of P and Fe treatments on seed germination and seedling vigor of winter wheat

    ZHANG Wanqing, DAI Mengya, HU Naiyue, ZHANG Yinghua
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.01
    Abstract:In order to clarify the effects of P and Fe treatments on the seed germination and seedling vigor of winter wheat, winter wheat cultivar ‘Jimai 22' was taken as study material. NaH2PO4 and Fe2(SO4)3 were used as the treatment solutions, and the maximum molar concentration which did not reduce seed germination rate was selected as the treatment concentration. The changes of dry weight, plant height, leaf area, SPAD and antioxidant enzyme activity of winter wheat seedlings under normal water(T1)and drought treatment(T2)at seedling stage were measured. The results showed that: Compared with the control, the germination rate of winter wheat seeds treated with 0. 05-0. 30 mol/L NaH2PO4 increased by 2. 00%-14. 00%, and the germination rate of winter wheat seeds treated with 0. 01-0. 40 mol/L Fe2(SO4)3 increased by 4. 01%. Compared with the control, the germination rate of winter wheat seeds treated with 0. 50-0. 90 mol/L NaH2PO4 decreased by 16. 00%-24. 00%, and the germination rate of winter wheat seeds treated with 0. 05-0. 07 mol/L Fe2(SO4)3 decreased by 10. 00%-24. 00%. Under the experimental conditions, the maximum concentrations of NaH2PO4 and Fe2(SO4)3 that would not inhibit seed germination and growth were 0. 30 and 0. 04 mol/L, respectively. Under the two water conditions, NaH2PO4 treatment significantly increased the dry weight and seedling height of winter wheat seedlings, while Fe2(SO4)3 treatment significantly increased seedling height only under normal water condition. Both NaH2PO4 and Fe2(SO4)3 treatments significantly increased the leaf area, SPAD, SOD and CAT activities of newborn leaves of winter wheat seedlings, but not POD activity. In conclusion, the appropriate concentration of NaH2PO4 and Fe2(SO4)3 treatments can promote the germination of winter wheat seeds, and NaH2PO4 and Fe2(SO4)3 treatments can improve the photosynthesis of winter wheat seedlings by increasing the effective photosynthetic area and chlorophyll content, and increase the activity of antioxidant enzymes in leaves, and then further enhance the seedling vitality and drought resistance.  
      
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  • Molecular cloning and expression analysis of small GTP-binding protein genes1 and8in cotton (L.)

    HU Ziyao, WU Xiaoyu, LEI Jianfeng, CHENG Guanfu, LIU Chao, DAI Peihong, LIANG Chunyan, XU Shijia, LI Yue
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.02
    Abstract:In order to explore the expression patterns of ROP genes in cotton under different abiotic stresses, GhROP1 and GhROP8 from Gossypium hirsutum L. were obtained by homologous cloning. Their physicochemical properties, structures and evolutionary relationships were analyzed by bioinformatics methods, and tissue expression specificity was explored by fluorescence quantitative PCR(qRT-PCR)technology and their expression patterns under different stress and exogenous hormone treatments were analyzed. The results show that: 1)The open reading frames(ORFs)of GhROP1 and GhROP8 genes are 591 and 630 bp in length, which respectively encodes 197 and 210 amino acids and both containing a conserved RHO domain. 2)Multiple sequence alignment result shows that the two GhROPs are conformed to have the structural characteristics of ROP proteins and has a high degree of homology with ROP proteins of other species. Cluster analysis shows that GhROP1 protein belongs to class I ROP protein, and GhROP8 protein belongs to class II ROP protein; 3)The two GhROPs genes are expressed in different tissues and have certain tissue specificity. The expression level of GhROP1 gene is the highest in leaves, and its expression level was lower in roots and stems. The expression of GhROP8 gene is the highest in roots and low in other tissues. The two genes are sensitive to abiotic stresses such as drought, high salt, low and high temperature and exogenous abscisic acid(ABA), auxin(IAA)all treatments have different degrees of response: The expressions of the two genes are induced by drought stress. The expression of GhROP1 gene is inhibited by high salt stress, while that of GhROP8 gene is induced by high salt stress; The expression of GhROP1 gene is inhibited by high salt stress, while GhROP8 gene is induced by high salt stress; Under low temperature treatment, the expression of two GhROPs genes decreased first and then increased; The expression of GhROP1 gene is down-regulated and then up-regulated under high temperature treatment, and the expression of GhROP8 gene is up-regulated and then down-regulated and then up-regulated; The expression of two GhROPs genes is inhibited by exogenous ABA; Under exogenous IAA treatment, GhROP1 gene is up-regulated first, then down-regulated and up-regulated again, and GhROP8 gene expression is induced by exogenous IAA. In conclusion, GhROP1 and GhROP8 genes may be important regulators of stress resistance in cotton, and this study provides a theoretical basis for further investigation of their biological function in cotton stress response.  
    Keywords:cotton;GhROPs;gene cloning;expression analysis;stress  
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  • Identification of soybeantranscription factor family and analysis of salt stress response

    CHEN Yibo, YANG Wanming, YUE Aiqin, WANG Lixiang, DU Weijun, WANG Min
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.03
    Abstract:In order to explore the function of soybean LIM transcription factor family genes in salt stress response, a total of 21 LIM genes, named GmLIM01-GmLIM21, were identified based on the whole genome data of soybean using bioinformatics method. The chromosomal location, physical and chemical properties, evolutionary relationship, conservative motif of soybean LIM gene family and its response to abiotic stress were analyzed. The results showed that: These GmLIM genes were not uniformly distributed on 14 of the 20 chromosomes in soybean, and fragment duplication was the main reason for the expansion of soybean LIM family. According to phylogenetic evolution analysis, the 21 LIM genes in soybean could be divided into five subfamilies. These 21 genes could be used to construct the evolutionary tree together with LIM genes identified in nine other plant species and divided into five subfamilies. The 21 GmLIM genes in soybean contained a total of 10 motifs, and most LIM proteins contained 3 different conservative motifs, namely, motif 1, motif 2 and motif 4. In addition, the analysis of cis-regulatory elements showed that the promoter sequence of the GmLIM gene was rich in regulatory elements related to light response, growth and development, plant hormones, and abiotic stress response. The quantitative PCR results showed that after salt stress treatment the expression levels of GmLIM07 and GmLIM17 genes in both leaves and roots reached peak at 3 and 6 h, respectively, and then decreased with the prolongation of treatment time. To sum up, GmLIM genes play an important regulatory role in the process of salt stress response in soybean.  
    Keywords:soybean;LIM;gene;bioinformatics;salt stress;gene expression pattern  
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  • Sequence and expression analysis of GDSL esterase/lipase gene in

    YAN Xiuxiu, FAN Bobo, ZHANG Xuefeng, YU Zhuo, FANG Yongyu, WU Jing, ZHAO Yan, MA Yanhong
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.04
    Abstract:In order to exploit GDSL lipase gene of Agropyron mongolicum, GDSL lipase genes were screened from the unigenes of A. mongolicum under drought stress. The physicochemical properties, subcellular localization, homology and phylogenetic evolution of the selected GDSL lipase gene were analyzed by bioinformatics methods. Meanwhile, the expression of GDSL lipase gene under different drought conditions was analyzed. The results showed that: A total of 52 GDSL lipase genes were screened from A. mongolicum. The protein size was ranged from 66 to 420 aa, the molecular weight was ranged from 6. 80 to 44. 77 kU, and the theoretical isoelectric point was ranged from 4. 27 to 9. 42. The 52 GDSL lipase genes were predicted to be distributed in extracellular, chloroplast, nucleus, cytoplasm, cell membrane and cell wall. Compared with 13 known GDSL lipase genes with drought resistance function and an evolutionary tree was constructed, the GDSL lipase gene of A. mongolicum has high homology with the GDSL lipase gene of rice, barley, arabidopsis, pigeonpea, soybean, wintersweet and pepper. Among drought treatments at 0. 5, 1. 0, 2. 0, 3. 0, 5. 0, 7. 0 d and rehydration 1. 0 d, 14 genes were significantly down regulated and 38 genes were up-regulated compared to control(CK). The prediction results of transmembrane, signal peptide and phosphorylation of six GDSL lipase proteins of A. mongolicum showed that DN26944_c0_g1 belongs to transmembrane protein, DN26944_c0_g1, DN14677_c0_g2, DN29240_c0_g2 all contain signal peptides, and it is speculated that they are exocrine proteins. Phosphorylation site analysis found that the phosphorylation degrees of DN26944_c0_g1, DN14677_c0_g2, DN29240_c0_g2, DN17531_c0_g6, DN2 9240_c0_g3, DN20405_c0_g1 protein were not high in general, among which DN26944_c0_g1 had 34 phosphorylation sites, and DN14677_c0_g2 had 7 phosphorylation sites. The differential expression analysis of the six GDSL lipase genes of A. mongolicum under simulated drought treatment showed that the expression levels of DN26944_c0_g1, DN14677_c0_g2, DN29240_c0_g2, DN17531_c0_g6, DN29240_c0_g3, and DN20405_c0_g1 were significantly higher than that of CK, indicating that the six GDSL esterase/lipase genes played a positive role in regulating A. mongolicum under drought stress.  
    Keywords:Agropyron mongolicum;GDSL esterase/lipase genes;bioinformatics analysis;drought resistance  
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  • Development situation analysis of biodegradable mulch film based on bibliometric analysis in China

    ZHAO Zijun, HE Wenqing, YIN Junhua, LIU Qin
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.05
    Abstract:In order to gain an in-depth understanding of the development status, research hotspots and development trends of biodegradable mulch film research, this research systematically sorted and screened the papers of the total biodegradable mulch film included in the CNKI database, Wan Fang database and Web of Science from 1990 to August 2022. Combining bibliometric analysis with Citespace method, this study visually analyzed the number of articles published in the field, main publishing institutions and test provinces, experimental crops and the highly cited literature. The results showed that: The number of articles published in this field displayed an upward trend, and the number of articles published in this field increased rapidly after 2011. From the perspective of major publishing institutions, Northwest A & F University, Chinese Academy of Agricultural Science and Shihezi University were the major research institutions with the highest amount of publication in this field; The main experimental provinces were concentrated in the northwest region, and the main crops were potato, cotton and peanuts. In conclusion, the application process of the biodegradable mulch film in the application process of the farmland is roughly the same as the ordinary mulch film in heat, preservation and crop yield improvement. The degradation of biodegradable mulch film is greatly affected by natural conditions, soil environment and its own composition, the future research on biodegradable mulch film in China needs to be strengthened.  
    Keywords:biodegradable mulch film;bibliometrics;research landscape;CiteSpace;application in farmland  
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  • Origin, classification and germplasm diversity in

    DU Fang
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.06
    Abstract:In order to provide a reference to the researchers and planters, and enhance preservation and utilization of lily germplasm, the species-level information on origin, classification, diversity in habitats, phenotype, genetics and function of Lilium in recent years are reviewed. The four breakthroughs in lily breeding are looked back. The study also presents a brief summary on existing problems in China on preservation and utilization of lily germplasm and developing trends in the future. The results showed that: 1)Lily cultivation in America appeared earlier than in Asia, and two Chinese letters pronounced “Bai He” first appeared in China and Asia. The origin of its modern lineage and the dispersal pathway along America and Europe had been tentatively explored. 2)Based on their morphological, cytological, molecular biology characters and breeding practices, the Lilium species are taxonomically classified into seven sections: Martagon, Pseudolirium, Lilium, Archelirion, Sinomartagon, Leucolirion, Oxypetalum. 3)Adapted to a variety of habitats, an extensive diversity was formed for Lilium on phenotype, genetics, and function. 4)Four breakthroughs had been made in lily breeding, including appearance of Asiatic hybrids, Oriental Hybrids, intersectional hybrids, and floral organ-specialized hybrids. In conclusion, an abundance of lily cultivars emerged, benefiting from the diversity resources. Future study should focus on the domestication of wild germplasm, take full advantage of lily resources in the world, and further develop the study of basic theory of molecular breeding in Lilium.  
    Keywords:Lilium;diversity;genome;classification;function  
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  • Tomato leaf disease recognition based on an optimized Swin Transformer

    LIU Yongmin, LIU Hanlin, SHI Tingting, OUYANG Jinyi, HUANG Hao, XIE Tieqiang
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.07
    Abstract:To timely and accurately recognize tomato leaf diseases, an optimized Swin Transformer method is proposed. A self-attention structure of Transformer is used to obtain the more complete high-level visual semantic information of tomato disease images. Mixup hybrid enhancement algorithm is combined to enhance image feature information in the preprocessing stage; In addition, transfer learning is adopted to train and optimize Swin Transformer model on enhanced tomato leaf disease data set, so as to achieve accurate tomato leaf disease identification. The results showed that: 1)With the optimized Swin Transformer model, the identification accuracy of tomato leaf disease reached 98. 40%; 2)Under the same training parameters, the accuracy of this model is 0. 70% to 1. 91% higher than that of the original Swin Transformer, VGG16, AlexNet, GoogLeNet, ResNet50, MobileNetV2, ViT and MobileViT models, and it could also rapidly convergence; 3)The Mixup hybrid enhancement algorithm added to the model greatly improved the identification accuracy of tomato leaf diseases, and had better performance and strong robustness than the existing common classical methods. Therefore, the new model proposed in this study can identify tomato leaf diseases more accurately.  
    Keywords:Swin Transformer;Mixup;data augmentation;tomato disease recognition;transfer learning;image classification  
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  • Effects of different LED light supply modes on the growth, photosynthesis and fluorescence of lettuce

    MA Liyuan, LI Yan, ZHANG Guanzhi, LI Yang, WU Yongjun, YANG Zhenchao
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.08
    Abstract:In order to explore the effects of combined photoperiod and duty cycle supplementary light source on the growth, photosynthesis and chlorophyll fluorescence parameters of lettuce, glass lettuce was used as the research material. By adjusting the optical period and duty cycle, T1(P16D25), T2(P16D50), T3(P16D75), T4(P8D100), T5(P12D100), T6(P16D100), T7(P20D100)and T8(P24D100)(P is the optical period; D is duty cycle. )were the 8 different light supply modes. The morphological indexes, biomass, pigment content, photosynthetic characteristics and chlorophyll fluorescence parameters of lettuce under each treatment were determined. The results showed that: The morphological indexes of lettuce under T8 mode were better than those of other modes. The actual photochemical quantum efficiency(Y(Ⅱ))of PSⅡ under T4, T5, T6, T7 and T8 modes was significantly higher than that of other non-100% duty ratio modes. The apparent photosynthetic electron transport rate of T1 and T2 was significantly higher than that of other treatments. There were no significant differences in biomass, pigment content, net photosynthetic rate, intercellular carbon dioxide concentration and stomatal conductance between T2 and T7 treatments. In conclusion, from the perspective of energy saving, T2 mode is considered to be the best and suitable for serving as the filling light source of lettuce.  
    Keywords:dailylight integral;LED;lettuce;duty ratio;photoperiod  
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  • WU Yi, ZHAO Pengfei, LI Xiaorui, Huangfumingke, CHEN Zhimeng, CHEN Hao, LI Chengjiao, BAO Siriguleng, BAI Zhiming, BAO Zhanying, LI Yu, Simujide, WANG chunjie, Aorigele
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.09
    Abstract:In order to compare the differences between Simmental cattle and their hybrid fattening cattle in blood indexes related to fat metabolism and meat quality, Simmental cattle(S group), Simmental cattle×Holstein cattle(SH group), Simmental cattle×Mongolian cattle(SM group)were divided into three groups, and the blood biochemical indexes related to fat metabolism and beef quality of the three groups of finishing cattle were determined. The results showed that: 1)The meat redness a* in SM group and SH group were significantly higher than those in S group(P<0. 05); 2)The glucose(GLU)of Simmental cattle×Mongolian cattle were significantly higher than those of Simmental cattle(P<0. 05); 3)The muscle yellowness b* of SH group was significantly lower than that of the other two groups(P<0. 01). To sum up, Simmental×Mongolian crossbred beef cattle have better energy and fat storage capacity than Simmental beef cattle, and also have better redness in meat color. This study can provide some data support and theoretical basis for beef cattle breeding and improvement and efficient production.  
    Keywords:Simmental;crossbred cattle;beef quality;lipid metabolism;blood index  
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  • miR-376b-5p regulated the expression of lambing trait related gene1in sheep

    LI Wentao, ZHOU Lijuan, LIU Ziyi, WANG Peng, CHU Mingxing, LIU Yufang
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.10
    Abstract:In order to explore the molecular mechanism of miR-376b-5p targeting and regulating IGF1 to influence the high-fertility lambing trait in sheep, five small-tailed Han sheep with high and low lambs were selected according to the lambing records. Ovarians and other six tissues were collected at follicular phase after simultaneous oestrus treatment. The expression of miR-376b-5p and IGF1 in ovarian tissues of high- and low-fertility sheep were analyzed by RT-qPCR, Western blot and dual luciferase reporter assay. And then the targeting relationship between miR-376b-5p and IGF1 was determined. Subsequently, the miR-376b-5p mimic and inhibitor were transfected into sheep primary granulosa cells in vitro to detect the expression of IGF1, and the expression of TGFβ1 and PTGS2, markers of the TGF-β signaling pathway, a pathway related to high-fertility laming trait, was also analyzed. The results showed that: 1)Tissues expression profiles showed that IGF1 was significantly higher in ovarian tissues of sheep than that in other tissues(P<0. 05). RT-qPCR and Western blot results showed that the expression of IGF1 in ovarian tissues of high-fertility sheep were significantly higher than that of low-fertility sheep(P<0. 01). The RT-qPCR results of miR-376b-5p showed that its expression trend was opposite to that of IGF1(P<0. 01). 2)The result of online software and dual luciferase assay showed that the miR-376b-5p could target binding to IGF1 3′UTR sequence. 3)IGF1 protein and mRNA expression levels were significantly decreased after overexpression of miR-376b-5p in sheep ovarian granulosa cells(P<0. 05), while the opposite was true after inhibition of miR-376b-5p, indicating that miR-376b-5p significantly inhibited IGF1 expression. 4)The expression of TGFβ1 and PTGS2 in the TGF-β signaling pathway was significantly lower than that of the negative control group after overexpression of miR-376b-5p in sheep ovarian granulosa cells(P<0. 05). In conclusion, miR-376b-5p targets the IGF1 3′UTR region in ovarian granulosa cells of sheep, and regulates granulosa cell proliferation and sheep reproduction by inhibiting IGF1 and TGF-β signaling pathways, providing a theoretical basis for further unraveling the molecular mechanism of sheep high-fertility lambing trait formation and molecular breeding.  
      
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  • Research progress on the coding RNA and non-coding RNA related to sheep reproduction

    LIU Zaixia, ZHANG Jingping, FU Shaoyin, HE Xiaolong, WANG Biao, He Jiangfeng, Baiyinbatu, HAN Chengzhi, MA Fengying, ZHANG Wenguang, SHI Caixia, LIU Yongbin
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.11
    Abstract:With the development and application of molecular biology and bioinformatics, many studies have screened and identified the coding and non-coding RNA related to sheep reproduction from the aspects of coding RNA and non-coding RNA related to the main reproductive activities such as ovarian development, ovulation, lambing, testicular development and spermatogenesis. In this study, the research of coding RNA and non-coding RNA affecting sheep reproduction were summarized, and 61 key mRNAs, 9 miRNAs, 17 lncRNAs and 4 circRNAs were sorted out. The development history and biological functions of RNA were further elaborated, and the coding RNA and non-coding RNA constituted a complex ceRNA network regulating sheep reproduction. The results showed that: 1)The mRNA of ram testis and ewe ovary at different ages showed dynamic changes during reproduction; 2)The interaction between non-coding RNA and DNA, RNA or protein regulates the processes of transcription, translation and post-translational modification, and realizes the regulation of spermatogenesis and follicular development in sheep of different breeds, ages and feeding levels. 3)The ceRNA regulatory mechanism formed by coding RNA and non-coding RNA, stable ceRNA regulatory mechanism makes the reproductive activity tend to a dynamic balance. This review aims to lay a foundation for excavating molecular marker genes of sheep reproductive traits, carrying out molecular breeding work, improving sheep reproductive performance and improving the economic benefits of the sheep industry.  
    Keywords:sheep;reproductive traits;coding RNA;non-coding RNA  
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  • Point cloud registration of sheep based on feature matching

    MA Xuelei, XUE Heru, ZHOU Yanqing
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.12
    Abstract:Aiming at the problem that the point cloud registration algorithm is easily affected by noise, outliers and sampling rate, the shape index key point detection method, nearest neighbor distance ratio method and iterative nearest point algorithm are adopted to study the point cloud registration method of sheep based on three-dimensional point cloud. The results show that: 1)The feature descriptor of covariance matrix can describe the key points detected by shape index method; 2)The registration algorithm based on feature matching can register point clouds of sheep at different view angles, and the maximum root mean square error is 0. 024 1; 3)The maximum root mean square error of registration is 0. 023 8 for different types of sheep point cloud models with noise, outliers or low sampling rate. The experiment proves that the registration method based on feature matching can accurately register sheep point cloud, and is not affected by noise, outliers and sampling rate.  
    Keywords:sheep;point cloud;point cloud registration;feature matching;distance constraint  
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  • Effects ofon anxiety-like behavior in mice with colitis induced by DSS and its protective effect on colon

    WANG Qiuzhen, DENG Ziteng, LAN Jing, LIU Xuelian, FAN Xiangwei, XUE Yi, MA Yunfei
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.13
    Abstract:In order to study the effect of Lactobacillus casei on anxiety-like behavior and colon protection in mice with acute colitis induced by dextran sulfate sodium(DSS), mice were divided into control group, DSS group, Lactobacillus casei group and Lactobacillus casei prevention group. The changes of body mass, colon length, intestinal epithelium, inflammatory infiltration, fecal occult blood in different groups were counted and compared by open field test and the changes of intestinal structure of mice in each group were detected by HE. The results showed that: 1)The distance of mice moving in the open field box and the time of staying in the central area showed a significant increase(P<0. 05). 2)Compared with DSS group, the length of colon and body mass of mice in Lactobacillus casei prevention group were significantly increased, the histological score and disease activity index were significantly decreased(P<0. 05), the structure of colon was more complete. In summary, Lactobacillus casei can reduce anxiety-like behavior in mice, alleviate the colonic damage induced by DSS in mice and play a protective role in acute enteritis.  
    Keywords:Lactobacillus casei;colitis;mice;behavior  
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  • Effects of hydrolysates made from silver carp processing by-products on the growth of probiotics

    ZHANG Huijuan, LIU Yueyue, LUO Yongkang, HONG Hui, TAN Yuqing
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.14
    Abstract:The purpose of this study is to explore a new way of high-value utilization of silver carp processing by-products and reduce the cost of nitrogen source for probiotic culture medium. The nitrogen recovery rate and relative molecular weight distribution were determined to explore the effects of different proteases(neutrase, alcalase, and papain)and hydrolysis time(0. 25, 0. 5, 1, 2, 3, and 4 h)on the properties of hydrolysates. The growth curve, specific growth rate, and total plate count of Lactobacillus rhamnosus LGG and Lactobacillus. acidophilus NCFM were determined to explore the effects of silver carp processing by-products hydrolysates on the growth and reproduction of probiotics. The results indicated that: Papain displayed a better hydrolysis effect on silver carp processing by-products. Specifically, the nitrogen recovery rate of hydrolysates obtained by papain was significantly(P<0. 05)higher than that of obtained by neutrase and alcalase at each time of hydrolysis. The proportion of components with relative molecular weight < 1 000 in hydrolysates obtained by papain(2, 3, and 4 h)was significantly(P<0. 05)higher than that of other hydrolysates. Hydrolysates obtained by neutrase(1 and 4 h), alcalase(4 h), and papain(1 and 4 h)replacing 75% nitrogen source in MRS medium accelerated the growth rate of L. rhamnosus LGG significantly(P<0. 05)and was higher than that of MRS medium. Hydrolysate obtained by papain(1 h)replacing 75% nitrogen source in MRS medium also significantly(P<0. 05)promoted the growth curve, specific growth rate, maximum OD600 value, and total plate count of L. acidophilus NCFM compared with MRS medium. In conclusion, the silver carp processing by-products are expected to replace the nitrogen source of the probiotic medium after hydrolyzation by neutrase, alcalase, and papain, and it can accelerate the growth and reproduction of probiotics, and realize its high-value utilization.  
    Keywords:silver carp;by-products;Lactobacillus rhamnosus;Lactobacillus acidophilus  
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  • QIE Lijuan, HAN Jianhui, LI Yong
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.15
    Abstract:Aiming at the problems of the unsatisfactory thermal insulation of wall, long time infrastructure and high construction cost in existing solar greenhouse, a solar greenhouse with assembled heterogeneous composite wall, which was energy-saving and environment-friendly, was designed in this study. Steel skeleton was assembled as the load-bearing system, and the wall was assembled on the skeleton with environment-friendly building materials that meet national standards. By taking the solar greenhouse with wall composited clay brick and phenolic plate as the control, an experiment was conducted to study thermal performance on the solar greenhouse with assembled heterogeneous composite wall in Shijiazhuang. The result showed that: The thermal resistance of the test greenhouse wall was 67. 2% higher and heat transfer coefficient was 40. 5% lower than that of the control greenhouse. The thermal inertia index of the test greenhouse wall was only about 1/3 of that of the control greenhouse, and the temperature stability zone of the north wall in the test greenhouse was obviously smaller than that in the control. Compared with the control greenhouse, on sunny day and cloudy day, the average daily effective heat storage of north wall in the test greenhouse were respectively 86. 3% and 94. 7% lower, and the daily heat release efficiency were respectively 23. 1% and 97. 7% higher. In the test greenhouse, the average indoor temperature for 3 sunny days and 3 cloudy days were 4. 9 ℃ and 2. 0 ℃ higher, respectively, and the lowest indoor temperature in the night were 4. 9 ℃ and 2. 0 ℃ higher, respectively. From 8: 30 to 10: 30 in 3 sunny days, the average temperature rise rate of the test greenhouse was 3. 1 ℃/h higher, it could provide the optimal temperature for the planted tomatoes as soon as possible and increase the photosynthetic products. In the night of 3 cloudy days, the average temperature fall rate of the test greenhouse was 0. 13 ℃/h higher. In conclusion, in the solar greenhouse with assembled heterogeneous composite wall, the insulation ability of the wall is significantly better, the indoor average temperature and the lowest temperature at night are higher, temperature rise rate are better in the sunny morning, which could meet the safe winter production of warm season vegetables, and provide a technical reserve for the upgrading of the solar greenhouse.  
    Keywords:solar greenhouse;assembled;heterogeneous composite wall;thermal performance;heat storage;heat release  
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  • Analysis on concept and theories of “Production-Living-Ecological” spaces

    DUAN Yaming, HUANG An, LU Longhui, JI Zhengxin, XU Yueqing
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.16
    Abstract:In order to comprehensively grasp the progress of the research of “production-living-ecological” space, “production- living-ecological” space is taken as keywords to search CNKI and Web of Science Core Collection. A total of 403 related papers published from 2012 to 2022 are obtained. On the basis of literature review, the concept, evolution mechanism, classification system, interaction relationship and development goal of “production-living-ecological” space are systematically summarized in combination with the theory of human-land relationship and the theory of multi-functionality of land use. It is also found that PLES is a coupled human and natural system with certain structure and function formed by the interaction between human activities and geographical environment, and its systemic development goal is sustainability, carbon neutralization and coordination. Production, living and ecological space also have different development goals, and include different sub-spaces respectively. There are obvious positive and negative effects between different spaces. Finally, the future research should pay more attention to the basic theoretical research of PLES(including the mechanism of formation, evolution and interaction, classification system, and development goal), the spatiotemporal change characteristic and driving mechanism of various sub-spaces within the PLES, the theories and technical system of quantitative measurement of the interaction between different spaces, the enrichment of the theories, methods and techniques of the optimization of PLES and the promotion of the parallel development of “production-living-ecological” space and “production-living-ecological” functions research.  
    Keywords:production-living-ecological spaces;formation mechanism;connotation;classification system  
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  • Research status and prospects of the cultivated land marginalization in China

    WANG Kechao, XIAO Wu, YU Chen, CHEN Hang, TAN Yongzhong
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.17
    Abstract:In order to review the research progress related to cultivated land marginalization, the study used the CiteSpace bibliometric analysis software. “marginalization”, “cultivated land+marginalization” and “land+marginalization” in both Chinese and in English were taken as keywords to retrieve China National Knowledge Infrastructure Database and Web of Science Journal Paper Database. A total of 154 Chinese papers and 427 English papers were obtained. Bibliometric statistical analysis and literature review methods were then used to systematically analyze the literature obtained through the framework of “definition-classification-driving mechanism-monitoring approach-impact evaluation”. The results show that: Cultivated land marginalization refers to the decline of the food crop production capacity of cultivated land, which is manifested in the form of abandonment, non-food transformation, and ecological retreat. Monitoring and evaluation of cultivated land marginalization include household surveys, statistical analysis, literature meta-analysis, and remote sensing extraction, and how to integrate macro-monitoring and micro-driven analysis is an important direction for the future. Existing studies generally focus on the negative impact of cultivated land marginalization on food security, and the comprehensive impact mechanism needs to be explored. The driving mechanism and impact of cultivated land marginalization have a large spatial heterogeneity, and subsequent research should focus on the zoned governance approach under multiple factors and scenarios.  
    Keywords:land resources;cultivated land marginalization;review;bibliometric visualization;driving mechanism  
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  • Spatial transfer characteristics and influencing factors of herbivorous animal husbandry in China

    HAN Chengji, WANG Guogang, ZHANG Yongxiang, Dong Haibin
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.18
    Abstract:Aims of this study are to investigate the spatial transfer and influencing factors of herbivorous animal husbandry industry and build a new type of planting and breeding relationship in different regions. Based on the panel data of 31 provinces/municipalities/ autonomous regions(the data do not include those of HongKong, Macau and Taiwan regions)from 1980 to 2020, the spatial distribution pattern, transfer characteristics and evolution process of herbivorous animal husbandry in China are systematically analyzed by using the standard deviation ellipse method. Based on the characteristics of industrial transfer, the spatial panel model is then used to reveal the influencing factors of industrial transfer. The results show that: The spatial dependence of herbivorous animal husbandry in China is significant, and the overall trend shifts from west to northeast. Further analysis shows that the industrial center of gravity crosses the dividing line of agriculture and animal husbandry as a whole, with a total linear displacement distance of 505. 36 km; The spatial panel model shows that the factors affecting industrial transfer are generally affected by six factors including the proportion of productive land, land production capacity, per capita GDP, Engel coefficient of urban residents, agricultural labor productivity and mechanization level. Among them, the land production capacity is the key factor promoting the transfer of herbivorous animal husbandry from pastoral to agricultural areas; In addition, different periods have their own emphasizes. The long time series is divided into three periods. It is found that mechanization, urbanization, water resource utilization efficiency and other factors are the main driving forces of industrial layout in each period. This study can provide scientific basis for the formulation of industrial structure adjustment policy in the herbivorous animal husbandry.  
    Keywords:herbivorous animal husbandry;influencing factors;spatial transfer;standard deviation ellipse;spatial panel model  
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  • GUO Jianbin, ZHANG Ying, ZHANG Zhiwei, HOU Lei, ZENG Weilian
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.19
    Abstract:The aim of this study was to explore the land use change and its driving mechanism in the alpine gorge of Tibetan plateau. Based on three periods(2010, 2015 and 2020)of Landsat satellite images, the spatial information of land use change in 30 m grid was obtained. The land use change characteristics and its driving forces were analyzed with socio-economic statistics, land use dynamic degree and geodetector. The results showed that: The area of grassland, forest, unused land and water slightly decreased from 2010 to 2020 in Nyingchi, while the cropland area increased in fluctuation, and the area of construction land significantly increased to 2. 66 times the original. The changes in land use types were concentrated in the flatland of the Yarlung Tsangpo River Valley, Nyang River Valley and Parlung Tsangpo River Valley. The dynamic degree of construction land change was at the highest level, and showed a trend of expansion year by year. The dynamic degree of cultivated land change was at the second, which displayed a trend of decreasing first and increasing afterwards. The comprehensive dynamic degree of land use was 0. 012% per year in Nyingchi. The comprehensive dynamic degree of Bayi district was the highest at county or district level, and the top three towns at town or township level were Bahe Town, Gengzhang Moinba Ethnic Township and Linzhi Town. In conclusion, the population density, grain yield, fixed assets investment, per capita net income of farmers and herders, urbanization rate, gross domestic product and elevation were the 7 dominant factors affecting the comprehensive dynamic degree of land use in Nyingchi. The land use change was the result of the combined effects of multiple factors. The explanatory power was enhanced after the interaction of various factors, among which the influence of socio-economic factors on the comprehensive dynamic degree of land use in Nyingchi was higher than that of natural factors.  
    Keywords:land use change;driving forces;geodetector;Nyingchi  
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  • Spatial dependence of soybean price and policy responses between China and major soybean import countries

    WU Jianzhai, HU Jiaxuan, XING Liwei, SHEN Chen, CHI Liang, ZHANG Jing, LIU Jifang
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.20
    Abstract:The quantity of China's soybean import has been large, and its price fluctuation has always been the focus of attention. In order to study the difference of price transmission in different soybean markets, based on the daily price of soybean markets in China, Brazil, Argentina, and the United States from January 2014 to May 2021, the study conducts an empirical analysis of the price dependence in the soybean markets between China and other countries by using wavelets and copulas. The results show that: The significant positive price dependence between China and other countries, especially Brazil, becomes much stronger with the expansion of the time scale. Moreover, for the different tail dependence coefficients in price transmission, negative shocks from Argentina and positive shocks from the United States are more likely to be observed after more than one month in China. In addition, an asymmetry price transmission(positive or negative)is usually found between China and Brazil/the United States instead of Argentina, which may change on the time scale for different countries. Finally, policy suggestions to increase the production of domestic oil crops, enrich import sources and realize market monitoring and early warning are put forward in this study.  
    Keywords:soybean price;spatial dependence;wavelet transform;Copula function;policy responses  
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    Updated:2024-02-27
  • HU Chenpei, HU Xia, JI Wei
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.21
    Abstract:The aims of this study are to clarify the agricultural economic growth mode of China since this century and its similarities and differences with other countries, and analyze the potential of China's agricultural economic growth in the future. Based on the neoclassical economic growth theory, the contribution of different production factors to China's agricultural economic growth since the beginning of this century are measured from the perspective of production factors, and the international comparisons of different growth models are conducted. The results show that: 1)From 2000 to 2019, the contribution rate of physical capital to China's agricultural economic growth reached 83. 6%, which is the most important driving force for agricultural economic growth; The contribution rate of total factor productivity to agricultural economic growth is 6. 2% and its level has increased. The contribution rates of land and fertilizers to agricultural economic growth are 5. 8% and 4. 4%, respectively. 2)International comparisons show that total factor productivity has become the most important driving force for agricultural economic growth in most countries, especially in developed countries and small-scale peasant economies and the agricultural economic growth in developing countries is still mainly driven by physical capital. 3)Through the comparison of different development stages, it is found that the growth momentum of agricultural economy will gradually be transformed from investment-driven to total factor productivity-driven, while the current agricultural economic growth in my country still shows a strong dependence on capital, and the level of total factor productivity will become a decisive factor key to long-term agricultural economic growth.  
    Keywords:agricultural economic growth;production factors;growth kinetic energy conversion;international comparison  
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  • Agricultural total factor productivity: Evolution process, calculation method and specific practice

    LIN Qingning, MAO Shiping
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.22
    Abstract:The aims of this study are to clarify the evolution process of agricultural total factor productivity, and solve the current problems such as unclear concept of agricultural total factor productivity, biased selection of measurement methods when calculating agricultural total factor productivity and unreasonable selection of input-output indicators. From the concept of agricultural total factor productivityr, the evolution process of agricultural total factor productivity is sorted out, and the main calculation methods of agricultural total factor productivity such as non-production frontier methods, production frontier methods and micro-accounting methods are summarized, and the advantages, disadvantages and applicable conditions of various methods are analyzed. The advantages, disadvantages and applicable conditions of various methods are described and the shortcomings of existing research and future prospects are put forward.  
    Keywords:agricultural total factor productivity;DEA;SFA;LP;OP  
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  • WANG Weixin, XU Jianghong, LU Wei, YIN Xukang
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.23
    Abstract:The aim of this study is to explore the solution of the “moving to city” phenomenon of Chinese rural surplus workforce, which is a typical problem among issues of agriculture, farmer and rural area in the era of digital economy. This research uses five phases of China General Social Survey(CGSS)data in 2010, 2012, 2013, 2015 and 2017 to systematically analyze the influence of internet use on agricultural workforce transfer decision and its function mechanism. The results show that: 1)The improvement of the utility level of internet use has significantly promoted the transfer of agricultural workforce to cities, and after a series of robustness tests, the results are still true. 2)Individual heterogeneity analysis discovers that internet use has a greater impact on the transfer decision between 30 and 60 and high-income agricultural workforce group; The regional heterogeneity analysis discovers that internet use has different impact on the agricultural workforce transfer decision in four main regions and five economic belts, and the driving effect in high-level digital economy development areas is stronger than that in low-level areas. 3)The mechanism analysis shows that internet use further affects the transfer decision of the agricultural workforce through the production effect, employment effect, channel effect and resilience effect. Therefore, this study puts forward the policy proposal of focusing on the “Four-in-One” of rural industry, rural production, rural service and rural construction digitalization.  
    Keywords:internet;agricultural workforce;transfer decision;transmission mechanism;CGSS  
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  • ZHANG Chengpeng, LI Mengqi, SUN Xiaolong, GUO Pei
    Vol. 28, Issue 4, (2023) DOI: 10.11841/j.issn.1007-4333.2023.04.24
    Abstract:To further release the dividends of rural land system reform and ensure food security, based on the survey data of 506 farmers in 2 cities and 6 counties in Shandong Province, Cobb-Douglas production function was used to explore the impact of “one household one plot” on grain yield per unit area. The results showed that: 1)Implementing “one household one plot” increased grain yield per unit area and it had a stable positive relationship with wheat and corn yield per unit area. 2)The implementation of “one household one plot” has different impacts on different peasant household groups. After the implementation of “one household one plot”, the per unit yield of wheat and maize of the peasant households with less agricultural work and non-agricultural labor at home has a greater increase. 3)Factors such as farmers' production and management training, farmers' cooperative membership status, and land quality of village groups have a positive effect on grain yield per unit area, and the grain yield per unit area of farmers who have participated in production and management training, members of cooperatives and high land quality are higher. Policy implications are proposed in this study as follows: The government should respect public opinion, encourage village groups to implement “one household one plot” according to local conditions and improve the food production conditions, increase grain production and achieve stable production and increase production.  
    Keywords:farmland fragmentation;one household one plot;grain yield per unit area;Cobb-doughlas production function  
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