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- Abstract：The current study is to provide powerful tools for the quantification of antiviral proteins(AVP : PKR,OAS/RNase L and Mx) in pigs.Primers were designed and synthesized based on the nucleotide sequences of PKR,OAS/RNase L and Mx genes available in GenBank.101 bp fragments were amplified by RT-PCR from the porcine peripheral blood mononuclear cells(PBMC),and then the fragments were cloned and sequenced.Recombinant plasmids were diluted by 10-fold serial and used as the Real-time PCR standard templates.Their s...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Established an indirect ELISA method for detecing PRRSV antibodies using the recombinant nucleocapsid protein(rN) of PRRSV expressed in E.coli(DE3) strain as antigen.Based on the ORF7 gene sequence encoding nucleocapsid protein of VR2332 strain in the GenBank,we designed a pair of primers and successfully amplified the ORF7 gene from PRRSV genome.The cloned gene was inserted into pET30a vector to obtain the recombinant prokaryotic expression plasmid.This recombinant plasmid was transformed into E.coli BL21(...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The distribution of endothelial and inducible nitric oxide synthase(eNOS,iNOS) were detected in 33,40,46,54 and 61 day old fetal porcine ovaries by immunohistochemical method,to investigate the role of nitric oxide on the germ cell during oogenesis.The result showed that positive immunoreactions for eNOS and iNOS were both present in the cytoplasm of surface epithelium,oogonium,oocytes,and necrotic oogonium or oocytes.The eNOS were also immunoreactive in the vascular endothelial cells.The eNOS immunoreactiv...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：To explore the fast and feasible method of cultivating endothelial cells(ECs) and smooth muscle cells(SMCs) from the pulmonary artery in chick embryo.Isolating and cultivating the pulmonary artery endothelial cells(PAECs) and the smooth muscle cells(PASMCs) from the chick embryo by tissue-sticking methods under the strictly sterile condition.The PAECs were sublimated after passaged when the monolayer confluence reached 80%.Two different morphologic characteristics and cultural character of the cells were ob...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The contents of protein,non-protein nitrogen and protein nitrogen were determined using GB/T5413.1,GB/T 21704-2008 and ISO 8968-5:2001/ IDF 20-5:2001 methods after adding melamine in milk,and the results were compared with the standard methods,the Biuret Spectrophotometry methord(B method) and Trichloroacetic acid and Biuret Spectrophotometry method(T+B method).It is shown from the results that the error rates were 46.60%,93.00% and 52.70% respectively for the methods of GB/T5413,GB/T 21704-2008 and ISO 896...2|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Arbors,shcrubs and herbage species were included for analyzing relations of different functional layers by using TWINSPAN and CCA.Results of TWINSPAN and CCA analyses were consistent.CCA ordination can reflect their spatial changes in graph of environment space.Results showed that the changes of aspect,slope and elevation can affect community type,structure and distribution of Carpinus turczaninowii forests and their functional layers,with the role of aspect and slope was more significant than that of eleva...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The objective of this research was to study the way of partner-hybrid breeding between high oil corn(HOC) hybrid and normal corn(NOC) hybrid.20 popular NOC hybrids were used as female to cross with 10 HOC hybrids varying in oil concentration to get 200 inter-hybrids.Based on the xenia performances of kernel weight and kernel oil concentration in inter-hybrid kernel after pollination,quality and yield traits such as oil concentration and kernel weight were analyzed.The results showed that the oil concentrati...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The Heilongjiang Province is an important base for marketable grain production in our country.For foodstuff security,it is essential to study the quantity and quality of cultivated land.Taking the cultivated land in Heilongjiang Province as the object of study,we analyzed and estimated the grain ultimate outputs,total cultivated areas,population data and so on between 1990-2007 in Heilongjiang Province by using the exponential model of minimum average cultivated area per person and cultivated land pressure....2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The present studies aim to discuss about the nitrogen balance circumstances in Tongzhou area,and to provide helpful data for optimized managing farmland nutrition and controlling pollution.By applying OECD surface soil nitrogen balance methodology,the framework and database for nitrogen balance budget in Tongzhou District,Beijing were established to evaluate the impact of nutrient balance on agricultural production from 1991 to 2004.The regional nitrogen balance intensity was analyzed with the help of GIS.T...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Allelopathy from winter wheat could stimulate germination of seeds of the parasitic plant,Orobanche minor and the germination rates may indicate the allelopathic potential of winter wheat developed in different historical periods.Distilled water and methanolic extracts of rhizosphere soil of winter wheat were used to stimulate the germination of O.minor seeds.The germination rates could reflect the suitability of winter wheat for planting in O.minor invaded fields as well as the allelopathic potential of wi...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：By means of anatomy and vertical slab polyacrylamide gel electrophoresis,cytology characteristics as well as isoenzymes of perioxidase(POD),catalase(CAT),esterase(EST) and amylase(AMY) of cytoplasmic male sterility(CMS) line RC7 and its maintainers have been analyzed.Results showed that microspore abortion occurred in the uninucleate pollen grain stage of CMS RC7.Difference of the number and intensity of the bands in POD and AMY zymograms was observed in flower buds at different stages of flower development...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Post-harvest quality and the activity of defense enzymes were analyzed in harvested Yali pear(Pyrus bretschneideri Rehd.) after exposure to an UV-C irradiation level of 5 kJ/m2.This dose of UV-C irradiation significantly inhibited disease development in fruits inoculated with Monilinia fructicola;the disease lesion diameter in mature pears irradiated with UV-C was 17% lower than in control.And the treatment delayed the decline in fruit firmness and soluble solids content(SSC) in Yali pear.The firmness of tr...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：To address problems of the continuously expanding area of cultivation of sweet cherries Prunus avium L.cv.Lapins) and consequent increases in yields as well as its short shelf life,we studied the Controlled Biological Freezing-point Storage(CBFS) for sweet cherries.The changes in quality,rot and juice freezing point of sweet cherry under different treatments of controlled freezing-point storage were investigated.Each of the CBFS treatments significantly maintained fruit firmness,soluble solids content,titra...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：An appropriate setting up of a landscape sculpture and its environment are prerequisites for harmonizing the landscape environment,which is also an issue to be researched.The effect of landscape environment on the sculpture cannot be ignored;an adequate representation of landscape sculptures is therefore a responsibility for both landscape designers and sculptors.In a sculpture-human-environment system,the psychological activity and behavior of spectators is the focal point,and the location of spectators is...3|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Twenty-four Liaoning cashmere goats were used to determine the effects of dietary protein levels(Low 10.5% DM,Moderate 12.1% DM and High group 13.5% DM) during the mating period on nutrient digestion and semen quality.The goats were assigned into 3 groups according to age and semen quality.The results showed that the total apparent digestibility of DM,CP,NDF and ADF did not differ significantly with increasing dietary protein levels.Although dietary protein levels had no significant effects on seminal quali...2|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：It is difficulty for the mechanized planting of rice to be applied in some regions of China,so a prototype of novel machine for producing rice seed rope was designed.It consisted of sending paper strip mechanism,measuring mechanism,twisting paper strip mechanism and rolling seed rope mechanism.Under the cooperation of these mechanisms,in factory rice seeds were dropped onto a paper strip of 4-7 cm width according to the required hill distance and the number of seeds per hill,then the paper strip with seeds ...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Improperly post-harvest drying causes lots of grain deterioration each year in China,because no match mobile drying equipment could be adopted in this interregional operation.To solve this problem,a new type of vehicle-mounted heat pump drying equipment has been designed.A horizontal multi-room fluidized bed is chosen as the drying chamber and a vapor compression heat pump(R134a as refrigerant) as heat supplier for this system.Latent heat of steam discharged from the forepart of the fluidized bed is recover...0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Biomass gasification provides an ideal solution for the energy conversion of agricultural and forestry residues with the end products as producer gas,liquid fuel,electricity and heat.CFB(circulating fluidized bed) was applied in this work as the gasification reactor for the test of rice husks gasification with air as the fluidizing and gasifying agent.Extensive tests were performed to investigate the effect of air equivalent ratio(ER) and gasification temperature on producer gas quality.The increase of ER,w...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Using air-impingement technology,the equipments of "Peking-duck" roasting and pretreatment roasting were developed to solve the potential products hazard to health in traditional "Peking-duck" roasting process.The optimal roasting processing,the sample pretreatment technology and the detection of PAHs(Polycyclic Aromatic Hydrocarbons) were studied.The technology of High Performance Liquid Chromatography-Fluorescence Detection(HPLC-FD) was used to detect three kinds of PAHs(Benzo [a] pyrene,Dibenz anthracene...1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
