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- Keywords：environmental microorganism,denaturing gradient gel electrophoresis(DGGE),PCR,protocol,ethanol precipitation methods,bacteria1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：Drosophila melanogaster,Cy mutation,genetic cross3|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Eye colour of Drosophila melanogaster is modulated by multiple genes,which is effective model for interaction research among genes.For understand the mechanism of mutation of the bright-red-eye progeny of Bar and wildtype fly,bright-red-eye mutants crossed to wildtype flies and eye colour mutants(cn,v and st) respectively.Eye pigment analysis of different fly lines was also performed.Results of crossing indicated that mutation of scarlet gene is responsible for the bright-red-eye phenotype.The bright-red-eye mutant was named st~(br) in this research.Results of eye pigment analysis represented that st~(br) fly have siginificantly less xanthommatin than wild flies(P<0.001) but no difference of drodopterin content is found between wild type and st~(br)(P>0.05).Bright red eye colour of st~(br) fly in this research may be result of mutation scarlet gene,which induced interruptive trans-membrane transportation of pigment precursor and decreasing biosynthesis of xanthommatin.Result of this research provides basis for further molecular biological analysis of st~(br) fly.Keywords：Bar eye,bright red eye,eye colour,eye pigment,Drosophila melanogaster1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：maize,haploid inducing line,parthenogenesis,inducing rate,doubling rate4|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to determine the components inheritance of quantitative resistance to stripe rust and the useful components in two wheat cultivars,seven populations derived from a cross of Libellula and Xian nong 4 which have resistance to Puccinia striiformis West.f.sp.tritici,were studied histopathologically.Heritability,degree of dominance and number of genes were estimated for these characters using genetic estimation.Causality of components and gene action of resistance were also studied using path analyses and joint scaling test.We found that colony length and width had higher heritability(0.79 and 0.57) and lower degree of dominance(0.02 and 0.11).The direct effect of the two components on the area of colony were 0.55 and 0.40 while the indirect effects were 0.45 and 0.32 respectively.These effects exceeded those of the others and only the two components fit the m[d] models.These results indicate that the length and width of colony could be useful among the five resistant components in breeding.Keywords：resistant component,inheritance,length and width of colony0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：Pseudoperonospora cubensis,enostrobilurin,baseline-sensitivity,resistance risk6|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to discuss the Quality Pig Breeding by quantity analysis approach,two bio-economic farm models for biological efficiency with or without Chinese Indigenous Swine were developed to conduct break-even,cost-structure and marginal profit analyses.The models were applied to a commercial pig farm with 600 sows including Chinese Indigenous Swine.Annual profits under three price assumptions(the same price as Lean Meat hog,lower price of local hybrid hog or higher price of Quality Pig) were respectively,-219.2,-913.9 and 93.87 thousand yuan.The break-even price was 8.256?yuan/kg and efficiency in net energy utilization and feed conversion was 31.58?MJ/kg and 3.534 respectively.The annual profit,efficiency in net energy utilization and feed conversion efficiency of the Lean Meat commercial pig farm of 600 sows was 560.1 thousand yuan,27.69?MJ/kg and 3.106,respectively.The results show that high price with high quality and reducing feed cost are the keys to Quality Pig Breeding.Keywords：quality pig,Chinese indigenous swine,bio-economic model,break-even analysis,marginal Profit0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Two experiments using high oil corn(HOC) stovers(variety Qingyou #1) were designed to examine the dynamic characteristics of ensilage fermentation(Exp.1) and to determine the effect of addition of silage slurry on rumen fermentation parameters of HOC stovers based on in vitro gas production method(Exp.2).In Exp 1,triplicate jars were opened at 1,3,6,9 weeks postfilling.In Exp 2,three treatments were used:(1) control(HOC silage distilled water),(2) HOC silage silage slurry,(3) HOC silage sterilized silage slurry.In Exp 1,more than half of WSC was used up within 1 week of ensiling.The pH dropped to 4.0 only after 1 week of ensiling.All nutrients of silage maintained stable after 6 week of ensiling.In Exp 2,addition of silage slurry fluid resulted in higher gas amount(PKeywords：high oil corn stovers,silage fermentation dynamics,silage slurry fluid,in vitro rumen fermentation,gas production3|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：An in vitro study was conducted to determine the effect of different types of fiber supplemented with sunflower oil on rumen fermentation and formation of conjugated linoleic acids(CLA) by mixed ruminal microorganisms.Cell wall components extracted from corn stover and beet pulp,and avicel were used as substrates,representing lignified fiber,easily digestible fiber and purified cellulose,respectively.Sunflower oil was supplemented at the same level(4.76% of substrate DM) for all three types of fiber.After 24?h of incubation,rumen fermentation parameters and the concentration of fatty acids(FA) in the culture fluid were determined.Results showed that the type of fiber significantly influenced(P<0.01) rumen fermentation traits in vitro.Composition of FA and profile of CLA were altered by the fiber type.Compared to the digestible fiber and purified cellulose,lignified fiber significantly increased(P<0.05) the production of cis9,trans11-CLA by rumen microorganisms.Keywords：conjugated linoleic acids,fiber type,rumen fermentation in vitro,2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：An in vitro study was conducted to study the effects of niacin addition to the dietary substrates on rumen fermentation and fibre degradation.The addition of niacin significantly increased total gas production,TVFA concentration,the activity of CMCase and Xylanase,and the degradation of DM and NDF(P<0.05),while did not significantly effect on the total counts of protozoa and bacteria,the potential gas production,the rate of gas production,pH,the ammonia-N concentration,the molar percentage of VFA,the ratio of acetate to propionate,the activity of Avicelase,and the degradation of ADF(P>0.05).The total counts of protozoa(P=0.082),the potential gas production(P=0.056),the activity of Avicelase(P=0.078),and the degradation of ADF(P=0.093) had an increasing tendency with the increase of niacin level.The current study suggested that the addition of niacin had beneficial effects on fibre degradation when the diet(fermentation substrate) contained higher corn stalk(70%).Keywords：niacin,rumen fermentation,fibre degradation1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The objective of this study was to observe the effect of Bingduke granules(Chinese medicinal herb) on Newcastle disease virus(NDV) in vitro.Cytopathic effect inhibition and MTT colorimetric assays were used to detect the anti-viral activity of Bingduke granules on NDV in chick embryo fibroblast(CEF) cells. The median toxic concentration(TC_(50)),median efficacious concentration(EC_(50)) and treatment index(TI) were 61.35?mg/mL,3.2?mg/mL and 19.2,respectively.The NDV was inactivated directly and the function of NDV infection in CEF was blocked by Bingduke granules in a concentration-dependent manner(P<0.01).Bingduke granules could effectively inhibit the replication of NDV when added to CEF cell within 9 hours at the dosage of 14?mg/mL after infection.These results show that Bingduke granules has significant inhibitory effect on NDV replication in vitro.Keywords：Newcastle disease viruses,antiviral effect,Bingduke granules,cell culture4|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The change of runoff coefficient in the time and space has become more and more complicated because of the diversification of the underlying surface of a city.The experiments for 5 typical underlying surfaces were carried out,that is,SBS,waterproof brick,cement road surface,permeable brick and grassplot by the artificial rainfall simulation system.The results show that the runoff coefficient is easier to reach a steady value for a poor infiltrate surface.The permeability of a permeable brick is close to a grassplot.Hyperbolic models had a higher precision to describe the relationship between runoff coefficient and single influence factor.The optimized simulation formulae of rainfall duration and runoff coefficient with certain physical significance were obtained.The rainfall duration has a significant effect on runoff coefficient and its correlation coefficient is as high as 92%-97% except for a permeable brick.Under the same condition of rainfall,the order of runoff coefficient of three poor infiltrate surfaces is waterproof brick,SBS and cement road surface in sequence.The proportion of low-lying detention and coherence water layer couldn't be neglected with a small rainfall and the permeability couldn't be reflected by runoff coefficient.The infiltration capacity of underlying surface in a city will be improved with the increase of the proportion of permeable brick and grassplot expanded.Keywords：rainwater utilization,runoff coefficient,rainfall intensity,underlying surface1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：astragalan polysaccharide(APS),extracting technology,phenol-sulfuric acid colorimetric method2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：leaf protein,lutein,alfalfa,extraction processing parameter2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：To study the characteristics of hydro-mechanical continuous variable transmission device,based on the hydro-mechanical continuous variable transmission(HMCVT) of a Dongfanghong 1302R tractor,the relation formulas of the speed ratio of HMCVT with displacement ratio of variable hydraulic pump(PV) and fixed hydraulic motor(MF),the hydraulic power distributing ratio and transmission efficiency were established.The power flow direction of each range(gear) was presented.The existing conditions of circulation power and its influence on mechanism output were analyzed.The analytical results showed that the characteristic parameter of planetary gear train k was the main design parameter that influenced the characteristics of HMCVT and its preference values located between 0.3 and 4.2;the setting principle of displacement ratio of PV and MF was that hydraulic power distributing ratio should be reduced as far as possible to ensure that HMCVT had a continuously variable speed ratio;in usual speed ranges of tractor,the absolute value of hydraulic power distributing ratio should be less than 0.2,which was beneficial to increase transmission efficiency;the existence of circulation power made transmission efficiency decrease,so the circulation power should be avoided;HMVCT had the characteristics of stepless-speed-regulation and high efficiency.The HMCVT device can meet the work requirements.Keywords：tractor,hydro-mechanical transmission,continuously variable transmission,characteristics analysis1|2|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to obtain the concentration of soil solution that has a linear relationship with the soil electrical conductivity(EC) for solute transport modeling a multipoint EC measurement system using the principle of four-electrode measurement method to determine EC was developed.The system hardware,including special designed small 4-pin probes,data acquisition boards(DAB),and temperature sensors,was integrated to compensate the temperature effect on EC.The monitoring software developed by VC programming language was adopted for collecting,displaying and storing functions of EC with the system hardware together.The consistency of multipoint measurements was resolved by calibrating to channels and probes.A series of experiments was carried out to reduce the effects from channels of DAB and sensors.The correlative coefficient between the measured and standard conductance is(0.999?5,) which shows that the experiments for calibration are necessary.While,the measuring errors from probes shouldn't be ignored and the structural constants of each probe should be calibrated section by section.Keywords：multipoint measurement,soil electrical conductivity,four-electrode,monitoring system2|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to relax the tension of urban water resources,expand the reuse of reclaimed water and ensure a good quality of urban lawn,the pot experiments of blue grass irrigated with reclaimed water were conducted.Seven irrigation treatments,proper irrigation with fresh water,proper irrigation with a ratio of 1 to 1 of fresh and reclaimed water,full irrigation with reclaimed water,proper irrigation with reclaimed water,light water stress irrigation with reclaimed water,medium water stress irrigation with reclaimed water and severe water stress irrigation with reclaimed water,were conducted.Their irrigation start-points are 70%,70%,80%,70%,60%,50% and 40% field water capacity(fc),respectively.For the seven treatments,irrigation amount for an irrigation event is the same.The experimental results showed that the leaf width irrigated with fresh water was narrower than that irrigated with reclaimed water;the tiller density irrigated with fresh water smaller than that irrigated with reclaimed water;the color rating irrigated with fresh water lower than that irrigated with reclaimed water and the rating of vegetational coverage and the rating of uniformity had the same variation law between treatments irrigated with different water qualities as the color rating.The ornamental quality was improved with the total irrigation water between treatments irrigated with the reclaimed water during the stages of turf life.The results showed that the ornamental quality under full irrigation with reclaimed water was the best estimated by the fuzzy comprehensive evaluation.The ornamental quality under proper irrigation with fresh water was worse than that under light water stress irrigation with reclaimed water.The ornamental quality under severe water stress irrigation with reclaimed water was the worst.Keywords：reclaimed water,Poa pratensis,ornamental quality,fuzzy comprehensive evaluation,4|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Keywords：characteristics curves,statistical analysis,hydraulic turbine,specific-speed0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The traditional evaluating methods can not deal with the evaluation problem of city atmosphere quality with fuzzy information.Based on the principle of structural risk minimization,authors constructed a support vector classification model,as well as its algorithm with fuzzy information and proposed a new city atmosphere quality evaluating method based on the fuzzy support vector machine.According to the four indexes,the total amount of suspending granules,sulfur dioxide,nitrogen dioxide and atmosphere synthetical pollution index,the atmosphere qualities of 50 main cities in China in the year 2003 were estimated.Keywords：support vector machine,fuzzy programming,air quality,assessment0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：Aiming at the situation that the simulating systems are very limited for policy simulation at present,a simulation system which makes use of computer technique is presented in this paper.The Visual C .net software was adopted to accomplish this integrate platform.The building and solving systems of CGE model were embedded into the GAMS software.The data warehouse was set up by SQL Server 2000 software.In this system,we integrated CGE model,DSS,data warehouse and data convert into one and established a prototype of the PSSBCGE policy simulation system.Through the scene analytical method,we simulated the agriculture subsidizes policy.The analytical results demonstrated that the prototype system had carried out some functions of the simulation system.In a word,this system can be used for the actual policy simulation problem and provide a decision support for decision maker.Keywords：policy simulation,computable general equilibrium model,DSS,system integrated3|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The existing methodologies for software interface design have a poor operating characteristic when used in the software interface design of the virtual instrument M&C system.In the paper,the interface distribution,pattern button,ActiveX animated simulation technology and matching colors were introduced and a GUI of M&C system for the complete equipment of seeds based on LabWindows/CVI and Human-Computer Interface discipline was designed.This GUI can monitor the equipment operation state and error signals directly,control the whole system and separate equipment,regulate the control law and parameter on-line,and make operation convenient and reduce fault operations.This approach can be used as a guideline in the interface design of the virtual instrument M&C system.Keywords：virtual instrument,measurement and control system,graphical user interface0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：In order to improve the quality of Laplace operator image segmentation at a high real time capability this paper presented a synchronization dimensional structure filter for Laplace operator.After constituting interconnected domain volume, a square threshold is introduced.Using the Run-Length coding method,implementing the synchronization of volume date-recording and Laplace difference,the filter keeps a high real time capability.The synchronization filter filtrates the thin square noise when the color threshold is depressed to obtain a more intact detection.The result indicates that the dimensional structure filter edge method can obtain a more intact and less noise image segmentation.The introduction of the synchronization dimensional structure filter makes the Laplace operator improve the segmentation quality with a high real time capability by the original arithmetic.Keywords：Laplace operator,dimensional structure filter edge,extraction0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：A cooling process for fermenting liquor was modeled and simulated using the Computational Fluid Dynamics(CFD) method.The calculated results for temperature field of the fermentation liquor were compared with those from the measuring values during a practical production process.The simulation results revealed the hydrodynamic and energy conditions inside a fermentation jar,which provided a solid fundamental for temperature control of a fermenting liquor during beer brewery.Keywords：beer fermentation,cooling process,Computational Fluid Dynamics,simulation0|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
- Abstract：The paper defines the connotation of intensive urban land use on the study of direct and indirect investment in urban land use.The result shows that any action that can improve the level of investment or heighten the efficiency of land use can be categorized as intensive urban land use.The evaluation index of urban land use intensity established three spatial scales(city,site and parcel,and comprises of three components) the level of investment in land use,the degree of land use and the efficiency of land use.The evaluation system can show different spatial locations and different uses of an urban land.Keywords：urban land,intensified use,evaluation index system1|1|0<HTML><DownloadPDF> <Meta-XML>Updated：2023-12-22
