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- Abstract：The RNA was extracted from purified beet necrotic yellow vein virus (BNYVV) isolated from Inner Mongolia. Heilongjiang and Ningxia. cDNA fragments corresponding to different RNA genome were cloned and sequenced with RT-PCR, Then compared the sequences between the different strain of overseas and the isolates of different region in China. The results showed that the "the triple block" of RNA2 between HU and F2, G1, S shares 97. 7%, 94. 9%, 96. 2%; and the 25 kD code region of RNA3 between HEI, NIN, BAO and HU shares 95. 4%, 93. 4%, 94. 0%. These sequence data demonstrated that RNA3 variants were widely existed (distributed) in the isolates of BNYVV which may be related with their pathogenicity.Keywords：beet necrotic yellow vein virus,RNA2,RNA3,sequence analysis0|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：From 1994 to 1996, investigation was carried out on mycoflora in staple corn from Linxian County Jingtoucun with high incidence of esophageal cancer. Results showed that Fusarium, Penicillium, Alternaria, Cladosporium, Aspergillus and Mucor were the common fungi. It was Fusarium that heavily contaminated in corn and F. moniliforme as well as F. proliferatum was the predominant species. In this three years the contaminated rate of Fusarium decreased year by year. However, its contaminated level was the highest one among the common fungi groups, and the store made contamination of the Fusarium more heavier in staple corn. It was also found that the difference between Jingtoucun and Beijing was not statistically significant.Keywords：esophageal cancer,mycoflora analysis,Fusarium0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：high pressure liquid chromatogram,fumonisin1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：resveratrol,resveratrol glucoside,red wine,HPLC9|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：24 strains of lactic acid bacteria, including 11 strians of Bifidobacterium, 10 strains of Lactobacillus and 3 strains of Cocci, were selected and their adhering abilities to colonic adenocarcinoma cell line HT-29 were tested individually in vitro. The results showed that the mean adhesive levels (organisms/cell) of Bifidobacterium, Lactobacillus and Cocci to cell line HT-29 were 10. 5, 3. 2 and 2. 3 respectively. The most highly adhering strian in Bifidobacterium to cell line HT-29 was B. bifidum 02 and the adhering level was 18. 4?. 7; the most highly adhering strain in Lactobacillus was L. acidodophilus 99101 and adhering level was 10. 2?. 8. In addition, the different strains in the same species expressed various adhering capacities, and no obvious difference in adhering abilities were observed between the lactic acid bacteria which belongs to the same genus from faeces of pig and human.Keywords：lactic acid bacteria,adhesion,HT-29 cells0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：According to the genetic loci of nisin-resistant (Nisr) linking to lactose-fermenting ability (Lac+) closely, four nisin-resistant Lactococcus lactis strains were directionally screened from 205 samples of fresh milk on a selective medium (M17) supplemented with nisin, lactose and bromocresol purple. The analysis of four strains demonstrated that they are Gram positive bacteria. The extraction of plasmid DNA illustrated that Lactococcus lactis subsp. lactis ZL2009, ZL2010, ZL2024, ZL2030 individually harboured 1,7,5 and 3 plasmids and they have different molecular weight.Keywords：Lactococcus lactis,nisin-resistant,directional screening,identification2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：China's sustainable development of agriculture is facing rigid challenges in 21st century. This paper analyzed the issue of sustainable food security that is uttermost important for China, based on the economic, ecological and social perspectives. It is pointed out that how to persist and raise peasants' incentives is of paramount importance for economic sustainability of agriculture. And the upgrading of efficiency of resources and the realization of substitute renewable resources for agro-inputs that are usually non-renewable, while the breaking of "deadlock" between agricultural intensification and the environmental degradation are regarded as the key rings. For these reasons, a strategy of "multiply green revolution" is proposed.Keywords：economic sustainability of agriculture,resources efficiency,substitution of non-renewable resources,multiply green revolution3|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In order to develop the native agro-specialities for the regulation of agricultural structure in China. Firstly, after the form factors analysis in the native agro-specialities, this paper developed the concept of suitability of native agro-specialities from the soil suitability that came from the traditional ideas of China into the modern land suitability in the world. So they called it as the modern land suitability of the native agro-specialities, and gave a definition as:"the modern land suitability of native agro-specialities is the applied technological science about studying the bio-physiological and ecological demand of the economic-biological characteristic in growth stage for the native agro-specialities as well as the corresponding technology of management". Secondly, this paper took these ecological demands of the economic-biologic characteristics of native agro-speciatistics into multicategories and regional divisions in geographical principle from habitat. The habitat ecological condition in maroclimate zone for general land suitability (SE4) to the quality ecological condition in micro climate and soil properties for excellent land suitability (SE1), till to the unsuitable condition(SEO) in some conditional calamities or soil properties. This system may be divided into five levels. It gave the systematic theorical illustration and feasible framework for the land suitability evaluation of the native agro-specialities, as the Chinese chestnut and orange were taken as the examples for systematic illustration. So itwas quite different from the frame for land evaluation in land suitability of FAO, because they took the methodology in the stepwise approachable method of soil geography. At last, in order to enhance the speed of regulation of agricultural structure and the scientific foundation of quality improvement in local agricultural products, this paper briefly introduced some methods and principles in land survey and land planning for land suitability.Keywords：native agro-specialities,economic-biological characteristics,land suitability science,land evaluation and land planning in land suitability,0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This paper discussed the methods of evaluating sustainable land management and selecting indicators and criteria for evaluation of sustainable land management. Although mathematics methods, such as the Principle Component Analysis, Correlation Analysis, Regression Analysis can be used to select indicators for evaluating sustainable land management. However, because of the complexity of land use system, the most effective way to select evaluating index is to utilize some practical knowledge as well as research results. Whether assessing the sum of those selected indicators values or appraising each single indicator from different view, both of them are reliable methods for evaluating sustainable land management.Keywords：sustainable land management,evaluating methods21|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A 6 ?10 NC ? diallel crosses were made by using 6 common wheat as female parents and 5 spelt wheats/club wheats as male parents ,heterosis of interspecific F1 hybrids on yield and quality were studied. The results showed that the interspecific hybrid of wheat had significant heterosis, the yield heterosis of F1 hybrids between common wheat and spelt wheat ranged from 43. 14% to 187. 96% with an average of 109. 24%, the average heterosis of spike number per plant and 1 000-grain-weight were high and obviously related to yield heterosis at 0. 01 and 0. 05 level, respectively; the average yield heterosis of F1 hybrids between common wheat and club wheat was 77. 19%(ranged from-2. 18% to 143. 42%), the average heterosis of spike number per plant and spike grain number were high and both was also obviously related to yield heterosis at 0. 01 level. In the quality test, the grain hardness heterosis over female parent was negative in most crosses but positive in crosses involved the variety Nongda 3226 as female parent, the level of protein content and wet gluten content were lower at 0. 01 level than that of its female parent in the interspecific hybrid involved club wheat, heterosis in sedimentation value were observed in most interspecific hybrids. It is concluded that the quality could be improved in some interspecific hybrid.Keywords：common wheat,spelt wheat,club wheat,heterosis,yield,quality27|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：winter wheat,summer maize,nitrogen fertilizer,residual effect1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：summer corn,N supplication strategy, 1 000-grain weight,grain crude proiten2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This paper discusses the image fusion method of TM multi-spectral bands and SPOT panchromatic band to improve urban expansion monitoring accuracy and the potency applied tochange detection. Firstly IHS transform is applied to finish TM multi-spectral bands and SPOT panchromatic band fusion, then MLC method is used to classify the fusion imagery; Finally theability of differing spectrum, texture and their combination applied to urban expansion extractionis compared. The result indicated that combination improves 21.87% and 10. 22% in user accuracy; a 14. 26% and 14. 12% in producer accuracy compared to spectrum only and texture only. For the whole classification, their combination improves 8. 47% and 17. 88% in overall accuracy; 0. 10 and 0. 21 in Kappa Coefficient. It is concluded that the fusion of high spatial imagery and multi-spectral bands can enhance change information, and the fusion of texture character can improve the classification results.Keywords：image fusion,urban fringe,texture character1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：According to the measured soil moisture under the optimal soil water and fertilizer conditions, crop water requirements (ETc) of three vegetables were calculated by soil water budget model. Crop coefficients (Kc) of cauliflower, amaranth and spinach during growth period were evaluated based on the determined ETc and reference evapotranspiration (ET0) by Penman-Monteith procedure. The crop water requirements for cauliflower, amaranth and spinach were 223. 8 mm, 144. 9 mm and 148. 1 mm separately; the mean Kc for above vegetables were 0. 68, 0. 94 and 0. 65 mm separately; the Kc value has a logarithmic function to leaf area index for experimented crops except for amaranth.Keywords：vegetable,crop water requirement,crop coefficient,field water budget,leaf area index0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Progresses on denitrification in rhizosphere and N2O emission were reviewed. N2O is an important intermediate product of soil denitrification. N2O production by denitrification not only depend on factors affecting denitrification rate, but also depend on factors affecting product ratio of N2O/N2. NO-3, C supply and O2 concentration in rhizosphere have complex effect on denitrification. Denitrification by rhizobium should be paid closer attention. N2O dissolved in rhizosphere water will probably be absorbed by plant roots and then released into the atmosphere through plant. Finally, several aspects for further investigation were indicated.Keywords：rhizosphere,denitrification,N2O2|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：There are five types of sensilla on the antennae of Locusta migratoria manilensis, namely, sensilla trichodae, sensilla chaetica, sensilla basiconica, sensilla coeloconica and sensilla cavity. Sensilla trichodae are slender and hairlike that inserted in the cuticle and only found on the surface of the scape and pedicel. Sensilla chaetica are similar but more robust and distributed uniformly on the antennae. Sensilla basiconia are also hairlike but very short and may be reduced to pegs or cones. The number of sensilla basiconica is 50 percents of the total number of the sensilla and they are concentrated on the antenna segments 10~20. On the other hand, the distribution of sensilla on the antennae of the locust infected with Nosema locustae is about the same as the healthy one. But the morphology of the sensilla has changed a little, mainly in the stripe on the surface of the antennae.Keywords：Locusta migratoria manilensis,antennal sensilla,type,structure,distribution21|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Investigations was conducted with 130 peach trees in 25 orchards at Feicheng County, 263 apple trees in 18 orchards at Taian, Dezhou, Yantai area in Shandong Province, and iron concentration in flowers and leaves were analyzed. The results showed that the iron concentrations and the range in peach and apple trees flowers were much higher than it in leaves sampled in May to June, July to August and October. The iron concentrations in peach flowers were correlated well with the iron concentration and chlorophyll content in leaves sampled in May and October, but not with July. Furthermore, active iron in peach flowers were also correlated well with active iron in peach leaves sampled in May and October, but not with that in July. There were good correlations between iron concentration in apple flowers and the iron concentrations and chlorophyll contents in leaves sampled at each time. It suggested that it was easily to distinguish iron deficient fruit trees from ironadequate ones with iron concentration in both flowers, and could be an good iron deficient diagnosing index for predicting iron supply in fruit trees.Keywords：peach,apple,flower,leaf,iron deficient chlorosis,diagnosing0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Influences of low temperature and poor light on some physiological parameters of tomato (Lycopersicon esculentum Mill. ) seedling were studied in illuminating incubator. The content of membrane-lipid peroxidation (MDA), activities of membrane-protecting enzyme (SOD), fluorescence parameter Fv/Fm of chlorophyll induction kinetic curves and endogenous hormones level were measured under low temperature and poor light stress after 7 days of treatment. Results showed that membrane-lipid peroxidation (MDA) increased and activity of membrane-protecting enzyme (SOD) decreased after low temperature and poor light stress. The fluorescence parameter Fv/Fm increased and CO2 assimilation efficiency decreased in a stress intensitg-dependence manner. The endogenous hormones level, including IAA, ABA, GA3, ZR, changed differently with stress treatments. These data indicated the difference between the varieties of tolerance to low temperature and poor light stress.Keywords：tomato,low temperature and poor light,activities of membrane-protecting enzyme (SOD),endogenous hormones,fluorescence parameter2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：A study was conducted to examine the effect of compensatory growth condition on the weight of breast and thigh meat, weight and length of intestines using broilers fed 22%, 20% , 18% and 16% protein diets at week 1, 2 or 3. The lower protein diet at any stage of week 1~3 increased 20~40 g of the thigh meat and 10~19 g of the breast meat as compared with the control diet (CP 22%). Meanwhile, the increased magnitude of the meat weight were larger in week 2 or 3 than week 1 when CP18%~CP16% diets were provided. Similarly, the CP18%~CP16% diets also had the tendency that the weight and length of intestine tract were increased. It is concluded that in the early growth, the lower protein diet could result in the increased weight of breast and thigh meats, and the difference of this effect is significant at week 2 or 3 when the dietary protein level is 18%~16%.Keywords：broiler,compensatory growth conditions,breast meat,thigh meat,intestine6|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This study was to investigate the relationship between amino acid availability and the hydrolyzed peptide quantity of poultry protein feed. In experiment 1, 25 three-month yellow-feather chicks were used to determine the amino acid availability of fish meal, soybean meal, cottonseed meal, rapeseed meal. The experiment result showed that the amino acid availability were 0. 92, 0. 87, 0. 75, 0. 76 for fish meal, soybean meal, cottonseed meal, rapeseed meal. In experiment 2, we determined the peptide amino acid (PAA) quantity in different protein, and studied the relationship between amino acid availability and peptide amino acid quantity. The result showed that the peptide amino acid quantity varied with different protein. There was a highest quantity of peptide amino acids with fish meal, followed by soybean meal, cottonseed meal and rapeseed meal . The more PAA quantity was hydrolyzed from the protein, the higher amino acid utilization ratio of the protein was. There was a strong relationship between the PAA quantity and amino acid availiability, and the highest relationship occurred between the PAA quantity hydrolyzed from the jejunum chyme collected after 6 hours and amino acid utilization ratio, R2 = 0. 98.Keywords：chick,peptide amino acid,amino acid availability2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This experiment was conducted to assess the influence of cecectomy on amino acid digestibility of three unconventional protein feedstuffs. Using Chinese yellow-feather broiler breeders. For cotton seed meal, the true digestibility of aspartic acid(P<0. 01), glycine(P <0. 01), and tyrpsine(P<0. 05) was significantly higher in intact birds than cecectomized ones, while on cystine in adverse. For rape seed meal, the true digestibility on valine (P< 0.05), phenylalanine (P < 0. 05) and lysine(P<0. 05) of intact birds was higher than cecectomized ones significantly, while the digestibility of glycine was in adverse. For poultry byproduct meal, the true digestibility of proline was higher in intact birds than in cecectomized ones significantly, while the digestibility of aspartic acid and lysine was in adverse. Intact birds showed a numerically higher value of average true amino acid digestibility than was cecectomized birds for the three unconventional protein feedstuffs. Cecetomized birds excreted more endogenous amino acids than intact birds (P>0. 05).Keywords：Chinese yellow-feather broiler breeders,cecectomy,amino acid digestibility,unconventional protein feedstuff4|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
