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- Abstract：In order to study the role of transcription factor GhWRKY41 in salt stress responsive progress of upland cotton (Gossypium hirsutum L.),a GhWRKY41 gene (Accession No.HM002635) was isolated from upland cotton by using RT-PCR and RACE methods based on SSH library analyze.The full-length of cDNA sequence is 1 630 bp,and has a 1 068 bp ORF with two introns,coding 355 bp amino acids.Transient expression assay showed that GhWRKY41 was subcellular localized in nucleus.Germination rate analysis showed that overexpression of GhWRKY41 gene could significantly increase the germination rate of transgenic cotton lines under abiotic treatments of drought,salt and cold stresses.The results of Real-time PCR proved that the GhWRKY41 gene could be induced by salt and drought treatments.And there were differences in the expression profile of GhWRKY41 gene in root,stem and leaf tissue.The expression level of GhWRKY41 gene after salt treatment was highest at 6 h in root,was highest at 48 h after treatment in stem,and was up-regulated at 6 and 24 h in leaves.The fiber quality traits of transgenic lines to receptor line were compared.And it was discovered that overexpressed GhWRKY41 could improve the lint percentage of transgenic cotton.In conclusion, GhWRKY41 was involved in plant response to salt and drought stress in cotton,and its overexpression in plant improved tolerance to salt and drought stresses.Keywords：upland cotton;GhWRKY41;salt stress;expression analysis;seed germination rate23|11|4<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To explore the adaptability and optimum sowing date of Quinoa in Beijing region,three sowing sites and four sowing dates were set in field experiment to investigate agronomic traits,dry matter accumulation,and yield performance of different materials.The results showed that Quinoa could reach maturity in 2014 and 2015.In 2014,the yield was 1 764.7 kg/hm2;In 2015,the average yield was 1 880.1 kg/hm2 of three experimental sites and the highest yield was 2 235.2 kg/hm2 in Daanshan,Fangshan.The rowing period ranged from 117 to 127 days in different sowing dates,plant height ranged from 185.1 to 197.5 cm,stem diameter ranged from 19.8 to 24.8mm,and first branch ranged from 20 to 27.Among different sowing dates,the yield of Quinoa ranged from 1 363.78 to 2 029.26 kg/hm2, grain weight per plant ranged from 27.86 to 33.20 g,thousand grain weight (TGW) ranged from 2.29 to 2.70 g.In conclusion,Quinoa was suitable to be planted in Beijing region,especially in Daanshan,Fangshan,which has cool climate and large temperature difference between day and night.The suitable sowing date was by the end of April.These results provided guidance for Quinoa planting in Beijing region.25|9|6<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The effects of different proportions red and blue light of 400 μ mol/(m2·s) light intensity on growth and photosynthetic characteristics of strawberry was studied.Taking ‘Benihope’ strawberry(Fragaria × ananassa Duch.)as material,different proportions of red and blue light,1:1 (Treatment A,TA),9:4 (Treatment B,TB),5:1 (Treatment C,TC),were generated by light emitting diode (LED) with fluorescent light generated light as control.The plant height,stem diameter and other plant morphological indexes of strawberry were investigated.The changes of chlorophyll content,indicators of photosynthetic,fluorescence parameters and morphological indexes of strawberry leaves were studied.The results showed that:1) The growth of plant fresh weight in TB was significantly higher than in other treatments and it was significantly increased by 38.4% with an increase of compared to the control group;the growth of stem diameter and leaf number of TB were significantly increased by 80.7% and 28.1%,respectively;2) TB had the thickest strawberry leaves,which was 14.3% thicker than the control;leaf area growth of TB was significantly higher than in TA with by 17.6%;3) The count of chlorophyll a in TB was largest and 11.11% higher than the control group;the count chlorophyll a/b of TB was the highest,and increased significantly by16.5%;4) With the enhancement of red light and weaken of blue light,the net photosynthetic rate,stomatal conductance,intercellular CO2 concentration and transpiration rate were gradually reduced;Fv/Fm in all treatments were lower than in control,ΦPSⅡ showed no obvious difference among treatments.In conclusion,when the total light intensity was constant,the ratio of red and blue light 9:4 helped the vegetative growth of strawberry,and resulted in stronger strawberry seedlings.Keywords：strawberry;red and blue light;LED;vegetative growth;photosynthetic characteristics27|8|8<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：SCF complex,thus regulating the ubiquitin degradation of organism protein.Two SKP1 family genes in cultivation strawberry, FaSKP1-1a and FaSKP1-1b,were cloned to analyze the function of SCF complex.The full-length of FaSKP1-1a and FaSKP1-1b nucleotide sequence shared the same size of 519 bp.Their nucleotide and amino acid similarity were 98.46% and 98.84%,respectively.A typical ‘GVDED’ tail structure was found in both FaSKP1-1a and FaSKP1-1b.Tissue-specific expression of FaSKP1-1a and FaSKP1-1b by RT-PCR and dCAPS showed that these two genes were highly expressed in root,leaves,stem,receptacle,pollen,style and fruit,but low in petal.These results can provide references for further research of the SCF complex in strawberry.Keywords：strawberry;FaSKP1-1;cloning gene;expression analysis25|8|4<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Taking Jiejiegua and Ribenxiaohuanggua as study object,digital camera and SPAD-502 were used to obtain the images and SPAD values of fixed leaf every three days.The color characteristic parameters and SPAD values of leaves were obtained and analyzed by using image processing techniques.A regression equation was also built.The results showed good linear correlation between NRI,H,DGCI parameters and SPAD values,where the R2 were 0.86,0.70 and 0.65 respectively.In conclusion,digital camera and image processing technique are usable in detecting the N nutrition in cucumber with advantages of fast speed,stable results,easy to apply,and non-destructive,and will provide references for crop nitrogen nondestructive diagnosis.Keywords：cucumber;nutrition diagnosis;digital image;nitrogen;SPAD23|10|5<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To provide theory basis of preventing walnut anthracnose in Shimian County,Yaan City,Sichuan Province,infected walnut leave,fruits and twigs samples were collected from this area.Culturing,separation and purification of pathogen were conducted.And themorphological characteristics,pathogenicity and the DNA sequence of ribosomal ITS of pathogen were analyzed for pathogen identification.Twenty-five fungicide formulas screening was determined by mycelial growth rate test and spore germination assay in laboratory.1)The results showed that:Nine isolates in total were morphologically identified According to their differences in colony morphology on the above and back side of PDA plate.These isolates were divided into two groups based on colony morphology,and isolates P51 and P57 belonged to each group were selected for follow-up analyses.The pathogenicity tests showed that symptoms caused by these two isolates were identical to those observed in field and the same pathogen was re-isolated from the diseased tissues.The biological characteristics indicated these isolates belonged to Colletotrichum gloeosporioides species complex.By sequence comparison with the related sequence in GenBank,it showed that the sequence homology was 100%.The result of phylogenetic tree indicated that isolates P51 and P57 were classified into one group of C.gloeosporioides including isolates AB981196,HQ645082,KC355249 and KJ676453.2)Fungicide screening:Five fungicides formulas including 100% streptomycin 3 000 times diluted strongly affected the growth of mycelium.Among which,four fungicides formulas were most efficient on exhibiting spore germination.100% streptomycin 3 000 times diluted,25% prochloraz 500 times diluted,50% thiram 500 times diluted,and 70% thiophanate-methyl 500 times diluted strongly affected both the growth of mycelium and exhibiting spore germination.This study suggested that the pathogen of walnut anthracnose in Yaan was C.gloeosporioides.And the best fungicides formulas were 100% streptomycin 3 000 times diluted,25% prochloraz 500 times diluted,50% thiram 500 times diluted,70% thiophanate-methyl 500 times diluted.Keywords：walnut anthracnose;pathogen identification;Colletotrichum gloeosporioides;fungicide screening23|9|4<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In order to better understand the biocontrol and plant growth-promoting functions of Bacillus cereus 905 and provide references for the genetic modification,the genome of strain 905 was sequenced and analyzed.The genome was sequenced by 454 pyrosequencing,and then analyzed using softwares for genome assembly,gene prediction and functional annotation,gene ontology (GO),cluster of orthologous group (COG) and synteny analysis.The result showed that the draft genome sequence of B.cereus 905 was about 5.39 Mb with a G+C content of 35.05%,consisting of 127 contigs.It has been deposited in DDBJ/ENA/GenBank under the accession no.LSTW00000000.Comparative genomic analysis showed that strain 905 had significant genomic synteny with other strains from B.cereus group.The genes related to plant growth promotion and biocontrol were discovered and predicted in the genome sequence. In this work,we report the draft genome sequence of strain 905,which will serve as a useful genetic reference for the strain optimization.Keywords：plant growth-promoting rhizobacterium;Bacillus cereus;biocontrol;genome sequencing;molecular mechanism20|8|1<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To establish Real-time PCR detection method of Neospora caninum in infected cattle,specific primers and TaqMan-MGB probes were designed according to N.caninum Nc2 conserved sequences.The size of PCR amplified fragment was about 150 bp and is consistent with the expected size;Real-time PCR amplification results showed that:The concentration of positive templates had a good linear relationship with the Ct value;The results were negative when detection positive DNA of Toxoplasma gondii,Theileria annulata,Babesia bovis and other parasites;Determination by 3D digital PCR,the lowest effective detection was 6.41 copy/μL,which was 1 000 times of the conventional PCR;The average coefficient variation between groups was 2.732% and was 1.108% within groups;And the detection result of this method if the was 100% (46/46) coincidence to the quarantine protocol for Neosporosis (SN/T 3499-2013).In conclusion,this method can be used for early diagnosis and routine monitor of livestock infected by Neospora caninum.Keywords：Neosporosis;Real-time PCR;3D digital PCR;Taq;Man-MGB probes;Nc2;gene20|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Tools of 16s Rrna (Illumina MiSeq sequencing platform and Qiime) were used to investigate the diversity of broiler's cecal microbiome.Chicken were raised in the same condition,15 healthy individuals were sacrificed to harvest their cecal samples at 22 days old.The results showed that:1) 359 158 sequences and 3 210 OTU were collected after sequencing and according to the Shannon-Wiener curve,the sequencing data was reliable for overall bacterial of the cecum.2) Differences mainly in sequencing number and the diversity of the microbiota were detected between samples.3) According to the core microbiota analysis,4 shared bacteria in phylum are Bacteroidetes(70.0%±12.1%),Firmicutes(25.4%±11.2%),Proteobacteria(4.3%±3.7%)and Cyanobacteria(0.3%±0.5%).4) Beneficial bacterial such as Lactobacillus,Bifidobacterium were detected in chicken cecum but the relative abundance was low by comparison.5) PICRUSt analysis was performed to show that abundance of the functional genes such as transporters,purine metabolism,DNA repair and recombination proteins,oxidative phosphorylation and methane metabolism were relatively high.Despite the differences among individual animals,lots of similarities were discovered in cecal microbiome among the samples.Keywords：chicken;cecal;microbiome;core microbiota;Illumina MiSeq sequencing;PICRUSt analysis24|8|8<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To explore sequences and structure of chicken methyltransferase like protein 5 (METTL5),the METTL5 was cloned by RT-PCR,and characterization of METTL5 was analyzed by bioinformatics methods.The expression patterns in different tissues and effects of folate deficiency in the maternal diets on METTL5 gene in offspring of broiler breeders were detected by real time RT-PCR.The results indicated that the METTL5 cDNA was 702 bp (Genbank accession:KU351684) in length,which encoded 212 amino acids.The phylogenetic tree analysis showed that the METTL5 of chicken was closely related to mallard and swan goose,but distant to African clawed frog.The METTL5 protein contained conserved AdoMet_MTases superfamily domain and was hydrophilicity protein.The secondary structure of METTL5 protein displayed that 44.81% of the METTL5 protein fold into α helix.This polypeptide contained no signal peptide in its amino acids,and was a nonsecretory protein.The residing probability of METTL5 in the cytoplasmic was 69.6%.mRNA expression levels of chicken METTL5 were detected by real time PCR in all 8 tissues investigated,and METTL5 mRNA expression level in abdominal adipose was the highest and in spleen was the lowest.Further qPCR analysis revealed that dietary folate deficiency in the maternal diets has a trend to increase the METTL5 gene expression levels in abdominal adipose of offspring of broiler breeders,but the difference was not significant compared to control group (P>0.05).Keywords：broiler;METTL5;gene;abdominal adipose;expression patterns21|7|2<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To explore the effect of exogenous androgen testosterone propionate on the expression of epididymis caput-specific glutathione peroxidase 5 gene (GPx5) in sheep,Enzyme-linked immunosorbent assay was adopted to detect the content of dihydrotestosterone (DHT) and testosterone (T) in serum and epididymal caput,and the expression level of androgen receptor (AR) protein in epididymal caput.Moreover,the relative expressions of GPx5-mRNA and AR-mRNA were measured by Real-time fluorescent quantitative PCR (RT-PCR);and GPx5 protein expression were analyzed by Western blotting.The results showed that:DHT in serum and T,DHT in the caput epididymidis were significantly increased to 0.5,6.0 and 0.8 folds (P<0.05),respectively;GPx5-mRNA and AR-mRNA expression in epididymal caput were significantly upregulated to 0.75 and 0.77 folds (P<0.05);the expression levels of GPx5 and AR proteins were also increased to 0.63 and 0.58 folds (P<0.05) in testosterone propionate treatment group compared with the control group.In conclusion,exogenous androgen testosterone propionate could promote GPx5 gene expression in sheep epididymal caput.Keywords：sheep;epididymidis;testosterone propionate;glutathione peroxidase 5;androgen receptor20|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To study the relationship between flattening coefficient and the moisture content changes of alfalfa main stem,a flattening testing device was designed,which provides adjustable spacing between the flattening rolls.Different parts of the main stem segments under different moisture content and flattening coefficients were tested.The water loss laws of alfalfa stems affected by different condition levels to different parts were studied.The results showed that:Under the conditions of temperature 25℃,relative humidity 50% RH,11 000 lx light illumination and 55% flattening coefficient conditions,moisture content changes in different parts of stem were basically the same;Although,the drying speed of alfalfa stem could be increased by using greater flattening coefficient in alfalfa field drying,and the stem water loss sensitivity was different in different parts by using different flattening coefficients.The stem closer to the root was more sensitive to different flattening coefficients,and its water loss rate was gradually significant increased with the decrease of flattening coefficients.The drying speed of the upper stems was not significantly speed up using flattening coefficient more than 70% (slightly flattening).Keywords：alfalfa;flattening coefficient;moisture content;matching24|8|2<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on land use status data in 1990 and 2008 land use dynamic degree[1],space transfer matrix and regression model were adopted to analyze the temporal and spatial characteristics of land use change and the correlation between land use dynamic degree and ground water level dropping speed in regionalization,The results show that:1) In addition to the increase in the number of construction land,the number of all other types reduce to some different extent in HHH plains;2) Each land use type's dynamic degree has a certain regularity in the regionalization,and the urban land use dynamic degree is the highest,indicating its fastest rate of expansion;3) Transformation of arable land to other land types is the main way of transformation,which is more obvious in the rapid dropping area.Arable land is the main source of construction land increasing,while ecological land become the main supplementary of arable land;4) The paddy field dynamic degree is negatively related to the ground water level dropping speed,and the urban land and rural residential land dynamic degree are positively related,indicating that the rapid expansion of urban land and rural residential land is a positive driving factor for dropping in groundwater level.To adjust and optimize the type and intensity of land use suitably in the ground water level dropping speed regionalization to safeguard regional ecological security and food security and achieve sustainable development in HHH Plains are recommended in this study.Keywords：land use change;ground water level dropping;spatial transformation;correlation;HHH Plains26|10|1<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：National agricultural science and technology parks play an important role in the construction of agricultural modernization.However,the efficiency of each park's land utilization is different.To obtain the overall efficiency,technical efficiency and scale efficiency of the national agricultural science and technology park,the integrated data of 28 national agricultural science and technology parks were collected and evaluated by using DEA method.The result shows that the proportions of the effective and ineffective comprehensive technique are the same.The core land area of the ineffective of agricultural science and technology park is substantially larger than 1 000 hm2,and the area of effective technology is about 400-700 hm2.In conclusion,the proper core area of national agricultural science and technology park is 500-1 000 hm2 and the agricultural science and technology park's science research investment should be enhanced.Keywords：agricultural science and technology park;data envelopment analysis (DEA);land use22|8|4<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on questionnaire investigation and mathematical statistics,the survey data of farm household in Kezuohouqi of farming pastoral area,farmers livelihood strategies and land utilization are explored.The results showed that:1) Agriculture is still the main source of livelihood,and there are obvious differences between farmers' livelihoods and land use under different natural conditions.Household type is mainly the type of combining planting and breeding.And the intensive degree of land input is very low in the area of poor soil and water conditions and Mongolian people gather.In the areas of good soil condition and abundant rainfall,the type of farmers is mainly planting,and the intensive degree of land inputs is significantly higher.2) Under different natural areas,farmers' choices of livelihood strategy have the same influence on land use,in other words,the intensity of land use degree has a significant tendency to increase with the increase of the proportion of household income.3) Farmers are likely to reclaimed land in the process of land using,especially in the area of poor natural conditions.In conclusion,In order to reduce the dependence on the natural environment and improve the livelihood level,the corresponding recommendations for different regions and objects are put forwarded.Keywords：farming pastoral zone;Kerqinzuoyihouqi;livelihoods;land use21|8|8<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：GIS technique and spatial autocorrelation tools are used to analyze the spatial-temporal pattern of the grain production and demand in Beijing-Tianjin-Hebei region from 1990 to 2010.Grain security index is built to identify grain security areas and investigate its changes.The results show that:1) The grain production in Beijing-Tianjin-Hebei region has experienced the following 3 phases of change since 1990,rising phase from 1990 to 1998,rapidly declining phase from 1999 to 2003 and re-increasing phase from 2003 to 2010;2) The counties with low grain yield reduce by year and those with medium grain yield remain stable.On the contrary,counties with high grain yield keep growing,which are mainly in the north part of the region;3) The result of grain production and demand analysis indicates the existence of spatial aggregation.While the southern plain becomes the hot spot of grain production and production-demand difference area where the grain production can fully satisfy its demand,the northern part is the cold spot in which the relationship between grain production and demand is quite tight.4) Most counties in the study area belong to safe in production and safe in demand category,followed by the category of safe in production-unsafe in demand.The unsafe in production and unsafe in demand category accounts for the least number of counties.Moreover,the classification of the changes in regional security shows that the majority of safe safe-safe counties locate in southern plain area,while the unsafe-unsafe counties are mainly distributed in the urban area of municipality and prefecture-level cities,the northern plateau,and mountain regions.In conclusion,in the view of cooperative development,complementary advantages of Beijing-Tianjin-Hebei region have important practical significance for the grain security of the whole area.Keywords：Beijing-Tianjin-Hebei region;grain production and demand;spatial and temporal pattern;security identification22|8|3<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on the panel data of 30 provinces during 2011-2012 in China,a Trans-log Production Function model of vegetable production was constructed,and the technological trends of Chinese vegetable and factors were analyzed.The results showed that there were the excess input in material expenses,cost of fertilizer and labor costs.There was obvious area and planter pattern differentiation in technical efficiency and the average technical efficiency of vegetable is 0.809 9 in China.And the six factors that influence China's vegetable production technical efficiency were examined by using data model.Finally,policy recommendations were put forwarded to improve Chinese vegetables production technology efficiency.Keywords：vegetable;protected vegetables;technical efficiency;frontier stochastic production function;factors23|9|3<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To reveal the livelihood status quo of farmers households in mountainous regions,analyzes of field survey data and comparison of livelihood capital and consequences for the livelihoods among farmer households with different livelihood strategies were conducted by taking Baoxing County,Sichuan Province as study object and using the sustainable livelihood analysis framework.The results show that:1) The impact of earthquakes on the livelihood capital of farmer households in Baoxing County is mainly reflected in physical capital and financial capital;2) The natural capital of farmer households with farming-dominated strategy is significantly higher than that of farmer households with non-farming-dominated strategy and non-farm strategy.While the human capital and physical capital of farmer households with farming-dominated strategy are significantly lower than that of farmer households with non-farming-dominated strategy and non-farm strategy.The difference of financial capital and social capital among farmer households with different livelihood strategies is not significant;3) The poverty incidence rate of the farmer households with farming-dominated strategy is the highest and farmer households with farming-dominated strategy brings more negative effects on the ecosystem and environment.Due to occupy higher natural capital,farmer households with farming-dominated strategy can choose characteristic agriculture,livelihood diversification,to participate in ecological compensation and other livelihood strategies.Keywords：farmer households;livelihood capital;livelihood strategy;consequences for the livelihoods;earthquake22|9|7<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Based on the 199 survey data of forest farmers in Chaoyang and Zhangwu of Liaoning Province,the forest farmer's behavior to adopt forestry technology is analyzed by using a binary logistic regression model.The results show that:1) 49% of forest farmers who plant econometric forest,and 88% of forest farmers who plant protection forest use forestry production technology;2) Social capital and attending technical training have positive effects on economic forest farmers using forestry production technology;3) Gender,household education years,forestry area affect the forestry production technology behavior of protection forest farmers significantly.Among them the householder gender and householder education years display negative influence;forest area shows positive influence.Attending technical training significantly influence the behavior of economic forest farmers and protection forest farmers,social capital significantly influence on the behavior of economic forest farmers,forest area has significantly influence on the behavior of protection forest farmers.Therefore,the government should strengthen the technical training for forest farmers,expanding forestry protection forest area and guiding the large forest farmers to play a leading role.Keywords：forest farmers;forestry production technology;economic forest;protection forest21|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Taking agriculture science as an example,the advantages of scientific research in high level universities of the world related to agriculture was thoroughly analyzed by using bibliometrics.The results show that:Four universities (Wageningen University,U.C Davis,Cornell University,China Agricultural University which were the top 4 universities in the U.S.News world university rankings in the field of agriculture science) have formed a certain size of high level of scientific research and innovation team,and brought together a number of leading figures;in the term of co-authorship,the scale of scientific research teams expanded every year,and the proportion of solo authors decrease year by year;The four universities all have given great importance to international collaboration;In the term of foundation,the four universities have access to different channels of funding supports,including funding from government,which is the main body.Meanwhile,the funding also showed a trend of socialization and diversification.This research provides intelligence clues for development decisions of first-class disciplines in scientific research management department of universities in China,and to provide references for the department to build world-class universities and first-class disciplines.Keywords：advantages of scientific research;agriculture science;world-class university;first-class disciplines;Web of Science;bibliometrics20|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
