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- Keywords：low temperature,broilers,activity of SR Ca 2+ ATP,Ca 2+ ion0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：rhizobia,16S rDNA PCR-RFLP,numerical taxonomy1|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：apple trees,IBN,cultivar,oxidation/reduction activity0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：chill,ethylene,NR gene,gene expression,physiological reaction3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：To investigate the involvement of Programmed Cell Death (PCD) in systemic response, tomato cells infected with TMV were studied. After inoculation with TMV on tomato leaves, the structure of shoot tip and leaf cells were studied with light and transmission electron microscope. The results showed that shoot tip cells appeared characteristics of programmed cell death (PCD): fewer cristae and broken double layer membrane of mitochondria, distorted proplastid grana lamellae, abnormal nuclei, condensed chromatin, abundant annulate lamellae and phenolics, multi vesicle bodies and swollen cell wall were observed in top cells. In addition, the structure of grana and stroma lamellae of chloroplast were disrupted in mesophyll cells. These results demonstrated that PCD was involved in the progress of systemic response, and the form of PCD in shoot tip cells might be similar to cytoplasmic cell death in animal cells.Keywords：TMV,systemic response,tomato,PCD5|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The effects of DTA-6, a new type of plant growth regulator, on the content of crude protein, essential amino acid and sulfur amino acid in alfalfa leaves and stems were studied, analysed, and the related mechanisms were inrestigated. The results demonstrated that DTA-6 increased the content of crude protein in leaf and stem by 39.2%, 33.5% respectively. In addition, DTA-6 increased the essential amino acid and sulfur amino acid in leaf, but decreased the content in stem. According to the results, DTA-6 had good effects on crude protein and amino acid content of alfalfa.Keywords：alfalfa,DTA-6,protein,amino acid1|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：ribosome mRNA complex,sucrose cushions,centrifugation liquid scintillation counting method2|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In this trial, effect of cattle feces and vermicompost on odor compounds in pig slurry were investigated. Two level of cattle feces and vermicompost were supplemented in anaerobically fermented pig slurry, and the effect of cattle feces and vermicompost on odor compounds in pig slurry were determined. It turned out that cattle feces had decreased the concentration of acetic acid and butyric acid by 7 fold and 2 fold respectively (P<0.01), except for propionic acid; The concentration of valeric acid and I valeric acid were increased in the slurry with cattle feces in mid stage of experiment, and deceased whereafter. In addition, cattle feces had significantly increased the biogas production by 3 fold (P<0.01), decreased the concentration of 3 methylindole in pig slurry. The concentration of p cresol and 3 methylindole in the slurry containing vermicompost was lower, compared with control; Vermicompost had also decreased the concentration of most VFAs in pig slurry. The concentration of total nitrogen, ammonium nitrogen were decreased significantly when the slurry supplemented with cattle feces and earthworm; Slurry pH and ammonia emission increased significantly in the slurry by cattle feces and earthworm supplementation. It was concluded that cattle feces and vermicompost can reduce some of odorant concentrations in anaerobically fermented pig slurry.Keywords：cattle feces,vermicompost,odorous compounds,pig slurry3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：Aspergillus 2TCiF2,rock phosphate,dynamic of phosphate solubilization0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：insect image,feature extraction,feature measuring,image recognition3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The theories and methods of risk analysis in plant protection were summarized according to conception of risk, risk analysis and pest risk analysis (PRA). Aim at the subject of data acquisition of PRA, integrate weather generator and frames of multi factor regulation of pests, ESR (Ecologically Simulated Reality) technology and ESR technology of PRA were built, and ESR growth chamber was designed. The technology can build system of ESR, carry out experiment of pest, data acquisition, establish model of pest and PRA, complete research of PRA. The technology can not only be used in PRA in plant protection, but also in other risk analysis of agriculture.Keywords：risk,pest risk analysis in plant protection,ecologically simulated reality2|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
Screening of actinomyces against Polymyxa betae and detection of effectiveness on rhizomania disease
Abstract：actinomyces strains were isolated from the surface and interior parts of lightly infected sugar beet roots, which were samples from heavily infected field. Three isolates were found to be effective on Polymyxa betae and identified as Streptomyces spp. Metabolic solution of the three strains could inhibit sprouting of resting spores and moving of zoospores of P. betae. ELISA of Beet Necrotic Yellow Vein Virus(BNYVV) showed that the number of BNYVV in sugar beet roots inoculated with Streptomyces spp. was less than that of the control. PCR showed that no specificity fragment of P.betae was amplified in sugar beet roots treated with Streptomyces spp., but there were the fragments amplified in that of the control. This study showed that the 3 Streptomyces spp.could inhibit Polymyxa betae, and the number of BNYVV decreased after treated.Keywords：Polymyxa betae,Actinomyces against P.betae,ELISA,PCR1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27- Abstract：To examine the effect of the 15% Hymexazol-Metalaxyl-Thiram seed coating formulation on watermelon seeds, this study was done on their germination, growth and development of the seedlings. The results showed that the germination rate of seeds, length and width of cotyledon and height of seedlings were not significantly different,compared with these of uncoated seeds. However, the length of roots was 15% longer, 22% more sideroots was observed in coated seeds and the weight of one hundred fresh seedlings rose 7%, after being treated. Moreover, accumulated content of Malondialdehyde(MDA) decreased by 59% and content of soluble protein increased by 17% through the coating treatment. Importantly, the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and polyphenoloxidase (PPO) in the seedlings were stimulated evidently, they were 46%,43%,31% respectively, and 57% higher than those of the untreated. It is concluded that the germination of watermelon seeds treated by the chemical was safe, the growth and development of seedlings were promoted and the activity of disease resistance-related enzymes was also increased after being treated.Keywords：15% Hymexazol Metalaxyl Thiram seed coating formulation,watermelon,growth and development,disease resistance related enzymes4|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The paper analyzed the development of sightseeing and tour fishery of cold water fishery industry integration in Huairou district in Beijing. 4 characteristics,3 advantages and 5 problems need to be solved were then summed up. Lastly the development strategies were proposed, including the richly utilization of cold water wealth, the comprehensive utilization and protection of sturgeon, the establishment of epidemic prevention system and the development of cold water fishery's ecological industry.Keywords：cold water fishery industry integration,sightseeing and fishing fishery,Beijing Huairou0|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：coated methionine,cashmere goat,nitrogen metabolism,cashmere1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：broiler,feed restriction,compensatory growth,protein turnover3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Two experiments were conducted using duodenal infusion method to investigate the effect of feed oligo peptides on portal absorption of amino acids in chickens under unanesthetized condition. The oligo peptides used in experiments were enzymatic hydrolysates of fish meal, soybean meal, cottonseed meal and rapeseed meal with the average molecular weight of <3kD and <1kD. Experiment 1: Portal Blood were collected at different minute after administration of enzymatic hydrolysate of soybean meal with the average molecular weight <3kD (S3). Plasma concentrations of free amino acids (FAA) were measured. The results showed that total plasma FAA concentrations immediately increased and reached maximum level at 20 min after infusion. Experiment 2: Absorptive intensity and balance were used to estimate the intestinal absorption rate of amino acids. FAA concentrations of portal blood at 20 min after duodenal administrations were determined. Amino acid absorption rates of eight infusion groups were compared. Results showed that amino acid absorption rates of <3kD groups were significantly higher than those of <1kD groups; Absorption rates of fish meal and soybean meal groups were markedly higher than those of cottonseed meal and rapeseed meal groups among <3kD groups or <1kD groups; There existed positive correlation between absorption rates and proportions of peptide bound amino acids (PAA) in infused substrates. The results of this investigation suggest that the fish meal and soybean meal are hydrolyzed into more oligo-peptides in animal gastrointestinal tract and consequently utilized more effectively by body tissues and had higher nutritive value.Keywords：chickens,feed oligo peptide,duodenal infusion,intestinal absorption,portal FAA0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This study was conducted to examine the effects of 0.4% tea polyphenols (TP) and 0.4% fructooligosaccharides (FOS) supplementation on performance and caecal microflora counts and its metabolites in female broiler chickens fed semi-purified diets from 28 to 42 day old. As the results, dietary TP and FOS significantly decreased mortality. abdominal fat pad weight and abdominal fat pad weigh/body weight at 42 day old compared to control group (P<0.05). In addition, the inhibitory effect for abdominal fat was larger in TP than in FOS (P<0.05). Dietary TP decreased significantly caecal total microflora counts and counts of each caecal flora population and production of caecal microflora metabolites compared to other groups (P<0.05). Dietary FOS not influenced caecal microflora counts but it selectively increased Bifidobacteri and Eubacteria (P<0.05), decreased other microflora (P<0.05) and concentration of caecal phenols and indole (P<0.0.5) compared to control. Results of this experiment suggest that in the semi-purified diets, TP and FOS have the effects of decreasing mortality, abdominal fat retention and changing caecal caecal flora population, but the TP showed the antibiotics like effects i.e. non selectively decreasing all caecal flora and its metabolites, and the FOS indicated the effects of selectively increasing probiotics and decreasing production of caecal flora metabolites besides volatility fatty acids.Keywords：broilers,tea polyphenols,fructooligosaccharides,performance,caecal microflora counts,caecal microflora metabolites1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：evening primrose oil (EPO),linseed oil (LO),fatty acid (FA),linolenic acid,DHA(docosahexaenoic acid),EPA (eicosapentaenoic acid)1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：blue peafowl breeders,energy level,egg production,fertility rate8|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：broiler breeder,avian encephalomyelitis,live vaccine,antibody4|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Microgametogenesis and its ultrastructures of Eimeria stiedai ((Lindemann, 1865)Kisskalt and Hartmann 1907) were studied with light and electron microscopy. Microgametogenesis can be divided into two phases. The first is the phase of growth and reproduction of nuclei of the gametocyte. As the central mass of cytoplasm, in which there are many nuclei, grows, it separates into several parts and the nuclei become situated around each part, forming the gametoblast. The second phase of microgametogenesis begins with the appearance of the rudiments of the microgametes on the surface of the microgametocyte. These are protuberances containing nuclei, mitochondria, and basal bodies. Further development is associated with a lengthening of the newly formed rudiment, nucleus, and flagella. The posterior end of the microgamete is attached to the cytoplasm of the gametocyte, and finally the entire microgamete buds off from the surface of the gametocyte. A mature microgamete, consisting of two flagella, one mitochondrium and one nucleus, has an elongate cresent shaped body and is enclosed by the unite membrane. The result showed that microgametogenesis of E.stiedai followed the general pattern of microgametogenesis of Eimeria spp.Keywords：Eimeria stiedai,microgametogenesis,ultrastructure0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
