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- Abstract：A total of 100 decamer oligonucleotides were used as primers to perform random amplified polymorphic DNA (RAPD) analysis on Triticum aestivum var. Chinese Spring and Thinopyrum intermedium. Out of these primers, one could amplify a specific DNA fragment in the accession SZ of Th. intermedium. This fragment, OPF03 1291 , was cloned and sequenced. Sequence data searching in GenBank showed that this fragment had a homologous degree of 88% to a RAPD marker OPF03 1296 (GenBank accession U43516) in Th. bessarabicum. Based on the sequence of OPF03 1291 , two pairs SCAR primers were designed. With OPF03 and primers P3 and P4, RAPD and SCAR analyses were performed on T.aestivum, T.aestivum Th.intermedium partial amphidiploid, Th.elongatum, Th.intermedium, wheat Th.elongatum substitution and addition lines. The results of PCR amplification showed that the RAPD marker was not appeared in materials with genome E e, whereas SCAR marker was appeared in all E genome possessed materials. The SCAR marker derived from the E b genome specific RAPD fragment could be used in the meantime to detect the chromosome of E e genome under common wheat background.Keywords：Thinopyrum spp.,genome,RAPD,SCAR8|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Eight qualitative binary characters, 47 qualitative polymorphic characters and 24 quantitative characters of 21 main gladiolus cultivars were observed on the field, in order to setup a scientific and reasonable DUS test guide. Uniformity, stability and distinctness of these characters were analyzed by using coefficient of variation, t test, and systematic cluster analysis. Results showed that the uniformity of all qualitative binary characters and most of qualitative polymorphic ones were good for all cultivars, while the quantitative ones were influenced by the environment significantly and their uniformity were bad. The stability of the qualitative binary characters were relatively good, while those quantitative ones were the worst. On the basis of uniformity and stability, we screened out 42 good characters, 26 relatively poor ones and 11 bad ones. And the first catesory of characters can be better used for comparing distinctness of cultivars, distinguishing one DUS test cultivar from another correctly and efficiently.Keywords：gladiolus,cultivar,distinctness,uniformity,stability1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：winter wheat,thermal requirement,development stage,leaf4|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The concept of the relative development stage ( RDS ) was adopted to account for the variation of the total thermal requirement for winter wheat and its leaf development in order to avoid the difference arose by the traditional method of accumulated temperature in the crop cycle. It was found that the RDS could express wheat development clearer than degree day, accumulated temperacture of traditional method and could greatly help to reveal the mechanisms of different factor's influence on wheat and its leaf development. The RDS was 0.47,0.59 and 0.69 repectively when winter wheat reach stem elongation stage, ear sprouting and filling stages. Without N fertilizer application in consecutive 5 rotating period, the relative RDS of three stages were increased to 0.52,0.62 and 0.75, means that the development of winter wheat is depressed seriously by nitrogen deficit. Similar phenomena were also found while water deficit or stress was serious. And the RDS of three stages were decreased to 0.39,0.52 and 0.64 if planting date was delayed by 20 days. Significant linear relationship was proven between leaf emergence represented by RDS and the leaf order on the stem, and so was the relationship between the time of leaf withering and RDS , if abiotic factors were phased out. The simulated leaf emergence rate indicates that leaf emergence is inhibited by salt, water and nitrogen stresses, whereas low temperatures caused from delayed sowing accelerated leaf emergence. Furthermore, the influence of daylength on crop development and leaf growth was evaluated, but no significant effect of day length was found in field. This might be attributed to the relatively small variation of daylength during the experimental periods.Keywords：winter wheat,crop development,thermal requirement,relative development stage( RDS ),leaf3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：winter wheat,genotype,soil N min,plant nitrate concentration8|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：fluo 3,boron,cytosolic Ca 2+ concentration,pollen germination4|11|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：Erdos,desertification,soil water,dune spatial variability4|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：In view of the complexity and multidimension of farmland system itself and the characteristics of blur existing in farmland evaluation, the study proposed a set of index system for farmland state evaluation that would be adapted to Quzhou county, Hebei. More over, by the use of Fuzzy synthetical evaluation method, the paper quantificationally assessed the state of Quzhou's farmland system . The conclusions reflected the county's status quo. fuzzy synthetical evaluation abandons the two valued logic , and it enables intermediate assessment between one state and anther state, so applying the method to complex system evaluation is more accurate,and the method offers a scientific mathematical framework to assess the farmland system.Keywords：farming system,index system,fuzzy synthetical evaluation3|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：Eco systematical structure change and its impacts on ecological degradation have been studied according to the 50 year historical statistics and research data in the Northern Ecotone. Now the population is 2.6 times, the cultivated area is 1.63 times, the number of small livestock is more than 10 times what they were in 1950. The excessively growing population is the root cause and the irrational eco systematical structure is the direct cause of the degradation in the eco system.Keywords：the Northern Ecotone,the eco system structure,ecological degradation4|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：herbicide resistant transgenic crops,glufosinate,biosafety1|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：herbicide resistance,transgenic rice,glufosinate8|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The progress of applied research on entomopathogenic nematodes(EPNs) in China was summarized. The strains in Steinernema carpocapsae, S.feltiae and Heterorhabditis bacteriophora have been mass produced for pest control in China. More than 10 recent selected strains belonging to Rhabditidae and Diplogasteridae were excellent biocontrol agents against pests. Three of them were produced for field use. More than 40 pests were tested with EPNs. The field control effect was 70% or more. Applying techniques, pest search strategies and desiccation tolerance of EPNs were influence factors for sieging pests in field. EPNs' sensitive and tolerance to chemical pesticides were discussed. Lower poison chemical pesticides could be mixed with EPNs for pest control. We suggested that EPNs could be used as bio monitors to inspect environmental pollution caused by chemical pesticides. The existed weak parts of research in China were pointed out.Keywords：entomopathogenic nematodes,strain selection,biological control,affect factors,poison tolerance,environmental pollution monitoring0|8|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：From 2000-2001,various parts of transgenic Bt cotton were tested with cotton bollworm larvae at different growing stages and investigated arthropod community structure in Yecheng,Xinjiang. The results were as follows. 1) Transgenic Bt cotton showed the highest resistance to cotton bollworm at the last tendays of May and July, which were 94.5% and 83.3%,but the resistance was the lowest in August(22.7%). 2) Different organs have different resistance to larvae of cotton bollworm, which was cotton bract(96.7%)? cotton bud(74.2%)?cotton petal(60%)?cotton leaf(50.2%)?cotton boll(30%)?cotton pistil(26.8%) on the first ten days of July. 3) Elder larvae have more ability to resist Bt cotton than younger larvae. 4) The diversity and evenness index were the common cotton without controlling any pest(0.7706 and 0.1883)?the Bt cotton field which utilized pesticides to control pest(0.3968 and 0.0931)?the Bt cotton field with natural enemies to control pests(0.2211 and 0.0549). The dominate concentration were Bt cotton field with natural enemies to control pests(0.9264)? Bt cotton field which utilized pesticides to control pest (0.8625)? the common cotton field(0.6881).So the stability of arthropod in the common cotton field was better than that of the transgenic Bt cotton field.Keywords：cotton bollworm,transgenic Bt cotton,toxicity expression,community3|7|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：engineered bacterium,Asian corn borer,insecticidal activity,Pseudomonas fluorescens1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The internal transcribed spacer 2s(ITS2)of ribsomal DNA of Trichogramma confusum, T. brassicae, T. evanescens, T. embryophagum and 6 geo populations of T. dendrolimi are cloned and sequenced. The identity and variation of ITS2 sequences between genera, species, and populations, and copies within an individual were then analyzed by bio software DNA Star. The results showed that the ITS2 identity between the genera of Trichogramma and outgroup was very low, whereas the ITS2 sequences between species of Trichogramma were moderately conserved. Our results also manifested that the ITS2s at intraspecies level or within an individual of Trichogramma were very conserved. These results suggested that ITS2 is a reasonable target region for molecular identification of Trichogramma spp.Keywords：Trichogramma,internal transcribed spacer 2 of ribosomal DNA,molecular identification5|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：Galleria mellonella L.,temperature,relative humility,population density,light,mass production2|9|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：This paper is a study on intercourse behavior of different hierarchy of Brandt's vole. The result is as follows: The aggressive behavior varies in different hierarchy or sexes, the high hierarchy male is the most intense. The possibility of being accepted varies, especially that of the low hierarchy is the highest. The strange female of low hierarchy has more chance to make its hierarchy lifting in the family.Keywords：Brandt's vole,social hierarchy,intercourse behavior1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Abstract：The economic weights of production traits are foundation information in swine breeding. In the study, a bio economic model was integrated to estimate the marginal profits of the goal traits which includes reproduction longevity (litter), litter size born alive (pig/litter), body weight at birth (kg), daily gain (g/day), feed conversion efficiency (kg/kg), dressing rate (%), lean meat percentage (%) and meat quality index (%). The results showed that in the default market and production condition, the marginal profits for the above goal traits were 1.64, 7.65, 4.23, 0.11, -99.00, 8.08, 10.69 and 7.13 RMB, respectively. The effects of market production conditions and genetic improvement on the marginal profits of these traits were also analyzed. The model can also be used to estimate the economic weights of the traits such as survivability, reproduction gap, conception rate, where it is difficult under traditional models.Keywords：swine,production trait,bio economic model,economic weight,marginal profit0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：Hu sheep,genotype estimation,phylogeny status1|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：Cryptosporidium parvum,mucosal immunity,systemic immunity,CP15/60 DNA,nucleic acid vaccine3|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
- Keywords：lactic acid bacteria,screening,Escherichia coli0|6|0<HTML><L-PDF> <Meta-XML>Updated：2024-02-27
