“Based on the triple helix theory and using the three party evolutionary game method, this study explores the impact of collaborative costs, collaborative benefits, collaborative incentives, and breach penalties on the evolution of the agricultural government industry university research collaborative innovation relationship among the three parties. It reveals the evolution law of the strategic choices of the government, agricultural enterprises, and research institutions in the agricultural government industry university research collaborative innovation, laying the foundation for the construction of the agricultural government industry university research collaborative innovation system.”